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Intratunical injection of autologous adipose stromal vascular fraction reduces collagen III expression in a rat model of chronic penile fibrosis

Hakim, Lukman ; Fiorenzo, Salvatore ; Hedlund, Petter LU ; Montorsi, Francesco ; Bivalacqua, Trinity J. ; De Ridder, Dirk ; Weyne, Emmanuel ; Ralph, David ; Garaffa, Giulio and Muneer, Asif , et al. (2019) In International Journal of Impotence Research
Abstract

Previous studies have shown that the injection of adipose stem cells and stromal vascular fraction(SVF) into the tunica albuginea (TA) during the inflammatory phase in a rat model of Peyronie’s disease(PD) prevented the development of TA fibrosis. Our aim was to investigate whether local injection of SVF can reduce established fibrosis in a rat model of chronic phase of PD. Eighteen-male 12-wk-old Sprague-Dawley rats were divided in three equal groups: sham, PD without treatment (PD) and PD treated with SVF(PD-SVF). Sham rats underwent 2 injections of vehicle into the TA one month apart. PD rats underwent TGF-β1 injection and injection of vehicle one month later. PD-SVF rats underwent TGF-β1 injection followed by SVF (1-million cells)... (More)

Previous studies have shown that the injection of adipose stem cells and stromal vascular fraction(SVF) into the tunica albuginea (TA) during the inflammatory phase in a rat model of Peyronie’s disease(PD) prevented the development of TA fibrosis. Our aim was to investigate whether local injection of SVF can reduce established fibrosis in a rat model of chronic phase of PD. Eighteen-male 12-wk-old Sprague-Dawley rats were divided in three equal groups: sham, PD without treatment (PD) and PD treated with SVF(PD-SVF). Sham rats underwent 2 injections of vehicle into the TA one month apart. PD rats underwent TGF-β1 injection and injection of vehicle one month later. PD-SVF rats underwent TGF-β1 injection followed by SVF (1-million cells) one month later. One month after the last treatment, the animals, n = 6 rats per group, underwent measurement of intracorporal and mean arterial pressure during electrostimulation of the cavernous nerve. Following euthanasia, penises were harvested for in-vitro study. Erectile function was not statistically significantly different between groups. PD animals developed subtunical areas of fibrosis and elastosis with upregulation of collagen III protein. These fibrotic changes were reversed after injection of SVF. We provide evidence that local injection of SVF reverses TA fibrosis in a rat model of chronic phase of PD.

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publication status
published
subject
in
International Journal of Impotence Research
publisher
Nature Publishing Group
external identifiers
  • scopus:85064530343
  • pmid:30988428
ISSN
0955-9930
DOI
10.1038/s41443-019-0136-9
language
English
LU publication?
yes
id
9672015e-dd63-4c39-8961-d8bd1c98b72e
date added to LUP
2019-05-07 14:07:02
date last changed
2023-03-23 06:47:42
@article{9672015e-dd63-4c39-8961-d8bd1c98b72e,
  abstract     = {{<p>Previous studies have shown that the injection of adipose stem cells and stromal vascular fraction(SVF) into the tunica albuginea (TA) during the inflammatory phase in a rat model of Peyronie’s disease(PD) prevented the development of TA fibrosis. Our aim was to investigate whether local injection of SVF can reduce established fibrosis in a rat model of chronic phase of PD. Eighteen-male 12-wk-old Sprague-Dawley rats were divided in three equal groups: sham, PD without treatment (PD) and PD treated with SVF(PD-SVF). Sham rats underwent 2 injections of vehicle into the TA one month apart. PD rats underwent TGF-β1 injection and injection of vehicle one month later. PD-SVF rats underwent TGF-β1 injection followed by SVF (1-million cells) one month later. One month after the last treatment, the animals, n = 6 rats per group, underwent measurement of intracorporal and mean arterial pressure during electrostimulation of the cavernous nerve. Following euthanasia, penises were harvested for in-vitro study. Erectile function was not statistically significantly different between groups. PD animals developed subtunical areas of fibrosis and elastosis with upregulation of collagen III protein. These fibrotic changes were reversed after injection of SVF. We provide evidence that local injection of SVF reverses TA fibrosis in a rat model of chronic phase of PD.</p>}},
  author       = {{Hakim, Lukman and Fiorenzo, Salvatore and Hedlund, Petter and Montorsi, Francesco and Bivalacqua, Trinity J. and De Ridder, Dirk and Weyne, Emmanuel and Ralph, David and Garaffa, Giulio and Muneer, Asif and Joniau, Steven and Albersen, Maarten and Castiglione, Fabio}},
  issn         = {{0955-9930}},
  language     = {{eng}},
  month        = {{04}},
  publisher    = {{Nature Publishing Group}},
  series       = {{International Journal of Impotence Research}},
  title        = {{Intratunical injection of autologous adipose stromal vascular fraction reduces collagen III expression in a rat model of chronic penile fibrosis}},
  url          = {{http://dx.doi.org/10.1038/s41443-019-0136-9}},
  doi          = {{10.1038/s41443-019-0136-9}},
  year         = {{2019}},
}