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Intratunical injection of autologous adipose stromal vascular fraction reduces collagen III expression in a rat model of chronic penile fibrosis

, ; Hakim, Lukman; Fiorenzo, Salvatore; Hedlund, Petter LU ; Montorsi, Francesco; Bivalacqua, Trinity J.; De Ridder, Dirk; Weyne, Emmanuel; Ralph, David and Garaffa, Giulio, et al. (2019) In International Journal of Impotence Research
Abstract

Previous studies have shown that the injection of adipose stem cells and stromal vascular fraction(SVF) into the tunica albuginea (TA) during the inflammatory phase in a rat model of Peyronie’s disease(PD) prevented the development of TA fibrosis. Our aim was to investigate whether local injection of SVF can reduce established fibrosis in a rat model of chronic phase of PD. Eighteen-male 12-wk-old Sprague-Dawley rats were divided in three equal groups: sham, PD without treatment (PD) and PD treated with SVF(PD-SVF). Sham rats underwent 2 injections of vehicle into the TA one month apart. PD rats underwent TGF-β1 injection and injection of vehicle one month later. PD-SVF rats underwent TGF-β1 injection followed by SVF (1-million cells)... (More)

Previous studies have shown that the injection of adipose stem cells and stromal vascular fraction(SVF) into the tunica albuginea (TA) during the inflammatory phase in a rat model of Peyronie’s disease(PD) prevented the development of TA fibrosis. Our aim was to investigate whether local injection of SVF can reduce established fibrosis in a rat model of chronic phase of PD. Eighteen-male 12-wk-old Sprague-Dawley rats were divided in three equal groups: sham, PD without treatment (PD) and PD treated with SVF(PD-SVF). Sham rats underwent 2 injections of vehicle into the TA one month apart. PD rats underwent TGF-β1 injection and injection of vehicle one month later. PD-SVF rats underwent TGF-β1 injection followed by SVF (1-million cells) one month later. One month after the last treatment, the animals, n = 6 rats per group, underwent measurement of intracorporal and mean arterial pressure during electrostimulation of the cavernous nerve. Following euthanasia, penises were harvested for in-vitro study. Erectile function was not statistically significantly different between groups. PD animals developed subtunical areas of fibrosis and elastosis with upregulation of collagen III protein. These fibrotic changes were reversed after injection of SVF. We provide evidence that local injection of SVF reverses TA fibrosis in a rat model of chronic phase of PD.

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International Journal of Impotence Research
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Nature Publishing Group
external identifiers
  • scopus:85064530343
ISSN
0955-9930
DOI
10.1038/s41443-019-0136-9
language
English
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yes
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9672015e-dd63-4c39-8961-d8bd1c98b72e
date added to LUP
2019-05-07 14:07:02
date last changed
2019-05-28 03:58:03
@article{9672015e-dd63-4c39-8961-d8bd1c98b72e,
  abstract     = {<p>Previous studies have shown that the injection of adipose stem cells and stromal vascular fraction(SVF) into the tunica albuginea (TA) during the inflammatory phase in a rat model of Peyronie’s disease(PD) prevented the development of TA fibrosis. Our aim was to investigate whether local injection of SVF can reduce established fibrosis in a rat model of chronic phase of PD. Eighteen-male 12-wk-old Sprague-Dawley rats were divided in three equal groups: sham, PD without treatment (PD) and PD treated with SVF(PD-SVF). Sham rats underwent 2 injections of vehicle into the TA one month apart. PD rats underwent TGF-β1 injection and injection of vehicle one month later. PD-SVF rats underwent TGF-β1 injection followed by SVF (1-million cells) one month later. One month after the last treatment, the animals, n = 6 rats per group, underwent measurement of intracorporal and mean arterial pressure during electrostimulation of the cavernous nerve. Following euthanasia, penises were harvested for in-vitro study. Erectile function was not statistically significantly different between groups. PD animals developed subtunical areas of fibrosis and elastosis with upregulation of collagen III protein. These fibrotic changes were reversed after injection of SVF. We provide evidence that local injection of SVF reverses TA fibrosis in a rat model of chronic phase of PD.</p>},
  author       = {,  and Hakim, Lukman and Fiorenzo, Salvatore and Hedlund, Petter and Montorsi, Francesco and Bivalacqua, Trinity J. and De Ridder, Dirk and Weyne, Emmanuel and Ralph, David and Garaffa, Giulio and Muneer, Asif and Joniau, Steven and Albersen, Maarten and Castiglione, Fabio},
  issn         = {0955-9930},
  language     = {eng},
  month        = {04},
  publisher    = {Nature Publishing Group},
  series       = {International Journal of Impotence Research},
  title        = {Intratunical injection of autologous adipose stromal vascular fraction reduces collagen III expression in a rat model of chronic penile fibrosis},
  url          = {http://dx.doi.org/10.1038/s41443-019-0136-9},
  year         = {2019},
}