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Tolerance (PICT) of the bacterial communities to copper in vineyards soils from Spain

Diaz-Ravina, M; de Anta, R. Calvo and Bååth, Erland LU (2007) In Journal of Environmental Quality 36(6). p.1760-1764
Abstract
To detect effects of Cu pollution, the Cu tolerance of soil bacterial communities extracted from several vineyards located in NW Spain was measured. Bacterial community tolerance was estimated by means of the thymidine (TdR) and leucine (Leu) incorporation techniques using either IC50 values (the log of the metal concentration that reduced incorporation to 50%) or the percentage of activity at one specific Cu concentration (10(-6) mol L-1 )The tolerance measurements by the TdR incorporation technique were similar to those obtained by the Leu incorporation method, indicating that the two methods were equivalent in terms of suitability for detecting the toxicity of Cu to soil bacterial communities. The two tolerance indices considered (IC50... (More)
To detect effects of Cu pollution, the Cu tolerance of soil bacterial communities extracted from several vineyards located in NW Spain was measured. Bacterial community tolerance was estimated by means of the thymidine (TdR) and leucine (Leu) incorporation techniques using either IC50 values (the log of the metal concentration that reduced incorporation to 50%) or the percentage of activity at one specific Cu concentration (10(-6) mol L-1 )The tolerance measurements by the TdR incorporation technique were similar to those obtained by the Leu incorporation method, indicating that the two methods were equivalent in terms of suitability for detecting the toxicity of Cu to soil bacterial communities. The two tolerance indices considered (IC50 values and percentage of activity) were closely correlated (r = 0.975, P < 0.001), showing that both were equally good in measuring Cu tolerance of the bacterial community. An increased bacterial community tolerance to Cu indicating a pollution effect, was observed in vineyard soils with more than 100 mg Cu kg(-1) soil. Thus, the long-term use of Cu in vineyards has a toxic effect on the soil bacterial community, resulting in an increased tolerance. An effect of increased levels of Cu could not be detected when measuring bacterial community activity, pointing to the increased sensitivity to detect toxicity in field studies using tolerance measurements. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Environmental Quality
volume
36
issue
6
pages
1760 - 1764
publisher
American Society of Agronomy
external identifiers
  • wos:000250972400023
  • scopus:36248997108
ISSN
0047-2425
DOI
10.2134/jeq2006.0476
language
English
LU publication?
yes
id
33724d21-e3e9-4c79-8a8f-eb035b807103 (old id 968622)
date added to LUP
2008-01-29 12:58:52
date last changed
2017-02-19 03:36:04
@article{33724d21-e3e9-4c79-8a8f-eb035b807103,
  abstract     = {To detect effects of Cu pollution, the Cu tolerance of soil bacterial communities extracted from several vineyards located in NW Spain was measured. Bacterial community tolerance was estimated by means of the thymidine (TdR) and leucine (Leu) incorporation techniques using either IC50 values (the log of the metal concentration that reduced incorporation to 50%) or the percentage of activity at one specific Cu concentration (10(-6) mol L-1 )The tolerance measurements by the TdR incorporation technique were similar to those obtained by the Leu incorporation method, indicating that the two methods were equivalent in terms of suitability for detecting the toxicity of Cu to soil bacterial communities. The two tolerance indices considered (IC50 values and percentage of activity) were closely correlated (r = 0.975, P &lt; 0.001), showing that both were equally good in measuring Cu tolerance of the bacterial community. An increased bacterial community tolerance to Cu indicating a pollution effect, was observed in vineyard soils with more than 100 mg Cu kg(-1) soil. Thus, the long-term use of Cu in vineyards has a toxic effect on the soil bacterial community, resulting in an increased tolerance. An effect of increased levels of Cu could not be detected when measuring bacterial community activity, pointing to the increased sensitivity to detect toxicity in field studies using tolerance measurements.},
  author       = {Diaz-Ravina, M and de Anta, R. Calvo and Bååth, Erland},
  issn         = {0047-2425},
  language     = {eng},
  number       = {6},
  pages        = {1760--1764},
  publisher    = {American Society of Agronomy},
  series       = {Journal of Environmental Quality},
  title        = {Tolerance (PICT) of the bacterial communities to copper in vineyards soils from Spain},
  url          = {http://dx.doi.org/10.2134/jeq2006.0476},
  volume       = {36},
  year         = {2007},
}