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Chromatography of living cells using supermacroporous hydrogels, cryogels

Dainiak, Maria LU ; Galaev, Igor LU ; Kumar, Ashok LU ; Plieva, Fatima LU and Mattiasson, Bo LU (2007) In Advances in Biochemical Engineering, Biotechnology 106. p.101-127
Abstract
The preparative cell separation is an intrinsic requirement of various diagnostic, biotechnological and biomedical applications. Affinity chromatography is a promising technique for cell separation and is based on the interaction between a cell surface receptor and an immobilised ligand. Most of the currently available matrices have pore size smaller than the size of the cells and are not suitable for cell chromatography due to column clogging. Another problem encountered in chromatographic separation of cells is a difficulty to elute bound cells from affinity surfaces. Application of novel adsorbents, supermacroporous monolithic cryogels, allows overcoming these problems. Cryogels are characterised by highly interconnected large (10-100... (More)
The preparative cell separation is an intrinsic requirement of various diagnostic, biotechnological and biomedical applications. Affinity chromatography is a promising technique for cell separation and is based on the interaction between a cell surface receptor and an immobilised ligand. Most of the currently available matrices have pore size smaller than the size of the cells and are not suitable for cell chromatography due to column clogging. Another problem encountered in chromatographic separation of cells is a difficulty to elute bound cells from affinity surfaces. Application of novel adsorbents, supermacroporous monolithic cryogels, allows overcoming these problems. Cryogels are characterised by highly interconnected large (10-100 mu m) pores, sponge-like morphology and high elasticity. They are easily derivatised with any ligand of choice. Convective migration can be used to transport the cells through the matrix. Target cells bind to affinity ligands, while other cells pass through the cryogel column non-retained and are removed during a washing step. Because of the spongy and elastic nature of the cryogel matrices, the cells are efficiently desorbed by mechanical compression of cryogels, which provides high cell viability and yields. The release of affinity bound cells by mechanical compression of a cryogel monolithic adsorbent is a unique and efficient way of cell detachment. This detachment strategy and the continuous macroporous structure make cryogels very attractive for application in cell separation chromatography. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
keywords
convective flow, affinity chromatography, cell separation, supermacroporous cryogels, monolithic chromatography, mechanical compression
in
Advances in Biochemical Engineering, Biotechnology
volume
106
pages
101 - 127
publisher
Springer
external identifiers
  • wos:000251354400005
ISSN
0724-6145
DOI
10.1007/978-3-540-75263-9
language
English
LU publication?
yes
id
110670a1-57b7-4f36-92bc-b085d186db03 (old id 968703)
date added to LUP
2008-01-29 14:13:37
date last changed
2016-04-16 04:52:09
@article{110670a1-57b7-4f36-92bc-b085d186db03,
  abstract     = {The preparative cell separation is an intrinsic requirement of various diagnostic, biotechnological and biomedical applications. Affinity chromatography is a promising technique for cell separation and is based on the interaction between a cell surface receptor and an immobilised ligand. Most of the currently available matrices have pore size smaller than the size of the cells and are not suitable for cell chromatography due to column clogging. Another problem encountered in chromatographic separation of cells is a difficulty to elute bound cells from affinity surfaces. Application of novel adsorbents, supermacroporous monolithic cryogels, allows overcoming these problems. Cryogels are characterised by highly interconnected large (10-100 mu m) pores, sponge-like morphology and high elasticity. They are easily derivatised with any ligand of choice. Convective migration can be used to transport the cells through the matrix. Target cells bind to affinity ligands, while other cells pass through the cryogel column non-retained and are removed during a washing step. Because of the spongy and elastic nature of the cryogel matrices, the cells are efficiently desorbed by mechanical compression of cryogels, which provides high cell viability and yields. The release of affinity bound cells by mechanical compression of a cryogel monolithic adsorbent is a unique and efficient way of cell detachment. This detachment strategy and the continuous macroporous structure make cryogels very attractive for application in cell separation chromatography.},
  author       = {Dainiak, Maria and Galaev, Igor and Kumar, Ashok and Plieva, Fatima and Mattiasson, Bo},
  issn         = {0724-6145},
  keyword      = {convective flow,affinity chromatography,cell separation,supermacroporous cryogels,monolithic chromatography,mechanical compression},
  language     = {eng},
  pages        = {101--127},
  publisher    = {Springer},
  series       = {Advances in Biochemical Engineering, Biotechnology},
  title        = {Chromatography of living cells using supermacroporous hydrogels, cryogels},
  url          = {http://dx.doi.org/10.1007/978-3-540-75263-9},
  volume       = {106},
  year         = {2007},
}