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Development of separation technique for stem cells

Kamihira, Masamichi and Kumar, Ashok LU (2007) In Advances in Biochemical Engineering, Biotechnology 106. p.173-193
Abstract
In recent years, human embryonic stem cells have been established, and somatic stem cells derived from various adult organs have been identified and characterized to differentiate into various kinds of functional cells. There have been attempts to use functional cells induced from such stem cells for tissue regeneration and cell therapy. The method is expected to become an important treatment for intractable diseases in the near future. Since tissues and organs generally contain only a small quantity of somatic stem cells, and since it is necessary to separate functional cells generated from stem cells for use in therapy, an effective method for specific cell separation is crucial to the practical application of regenerative medicine. For... (More)
In recent years, human embryonic stem cells have been established, and somatic stem cells derived from various adult organs have been identified and characterized to differentiate into various kinds of functional cells. There have been attempts to use functional cells induced from such stem cells for tissue regeneration and cell therapy. The method is expected to become an important treatment for intractable diseases in the near future. Since tissues and organs generally contain only a small quantity of somatic stem cells, and since it is necessary to separate functional cells generated from stem cells for use in therapy, an effective method for specific cell separation is crucial to the practical application of regenerative medicine. For the specific separation of cells, a fluorescence activated cell sorter using specific antibodies is a powerful tool, but the method is not suitable for large-scale processing and a special device is required. Although a magnetic cell separation system using immuno-magnetic fine particles is also commercially available, the system still needs special apparatus for large-scale processing. We developed a novel method for the separation of specific cells in an aqueous two-phase system using antibodies modified with a temperature-responsive polymer. The method enables the processing of a large quantity of cells without the requirement of a special device. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
stem cell, specific cell separation, poly-N-isopropylacrylamide, aqueous two-phase system, immunoaffinity partitioning
in
Advances in Biochemical Engineering, Biotechnology
volume
106
pages
173 - 193
publisher
Springer
external identifiers
  • wos:000251354400008
  • scopus:54849422598
ISSN
0724-6145
DOI
10.1007/10_2006_043
language
English
LU publication?
yes
id
09d7576e-932d-44dd-8468-5a970782f65d (old id 968709)
date added to LUP
2008-01-29 14:46:57
date last changed
2017-03-19 04:11:02
@article{09d7576e-932d-44dd-8468-5a970782f65d,
  abstract     = {In recent years, human embryonic stem cells have been established, and somatic stem cells derived from various adult organs have been identified and characterized to differentiate into various kinds of functional cells. There have been attempts to use functional cells induced from such stem cells for tissue regeneration and cell therapy. The method is expected to become an important treatment for intractable diseases in the near future. Since tissues and organs generally contain only a small quantity of somatic stem cells, and since it is necessary to separate functional cells generated from stem cells for use in therapy, an effective method for specific cell separation is crucial to the practical application of regenerative medicine. For the specific separation of cells, a fluorescence activated cell sorter using specific antibodies is a powerful tool, but the method is not suitable for large-scale processing and a special device is required. Although a magnetic cell separation system using immuno-magnetic fine particles is also commercially available, the system still needs special apparatus for large-scale processing. We developed a novel method for the separation of specific cells in an aqueous two-phase system using antibodies modified with a temperature-responsive polymer. The method enables the processing of a large quantity of cells without the requirement of a special device.},
  author       = {Kamihira, Masamichi and Kumar, Ashok},
  issn         = {0724-6145},
  keyword      = {stem cell,specific cell separation,poly-N-isopropylacrylamide,aqueous two-phase system,immunoaffinity partitioning},
  language     = {eng},
  pages        = {173--193},
  publisher    = {Springer},
  series       = {Advances in Biochemical Engineering, Biotechnology},
  title        = {Development of separation technique for stem cells},
  url          = {http://dx.doi.org/10.1007/10_2006_043},
  volume       = {106},
  year         = {2007},
}