Detection of Helicobacter species in chronic liver disease and chronic inflammatory bowel disease
(2007) In Annals of Medicine 39(7). p.554-560- Abstract
- Aim. To study the association between helicobacters and chronic liver diseases and chronic inflammatory bowel diseases. Patients and methods. Thirty-two patients with various chronic liver diseases and 137 patients with inflammatory bowel disease were enrolled. Antibodies to H. pylori, H. hepaticus, H. bilis, and H. pullorum were measured by enzyme immunoassay (EIA), and sera positive in a non-pylori helicobacter EIA were further examined by immunoblot assay. Detection of Helicobacter DNA in liver biopsies was done by denaturating gradient gel electrophoresis of PCR products (PCR-DGGE) and DNA sequence analysis. Results. Six inflammatory bowel disease patients, four with ulcerative colitis and two with Crohn's disease, and one liver... (More)
- Aim. To study the association between helicobacters and chronic liver diseases and chronic inflammatory bowel diseases. Patients and methods. Thirty-two patients with various chronic liver diseases and 137 patients with inflammatory bowel disease were enrolled. Antibodies to H. pylori, H. hepaticus, H. bilis, and H. pullorum were measured by enzyme immunoassay (EIA), and sera positive in a non-pylori helicobacter EIA were further examined by immunoblot assay. Detection of Helicobacter DNA in liver biopsies was done by denaturating gradient gel electrophoresis of PCR products (PCR-DGGE) and DNA sequence analysis. Results. Six inflammatory bowel disease patients, four with ulcerative colitis and two with Crohn's disease, and one liver disease patient with autoimmune cholangitis had antibodies to non-pylori helicobacters by an immunoblot assay. Four immunoblot assay-negative patients, three with autoimmune and one with non-autoimmune liver disease, had Helicobacter DNA in liver biopsies; three of the polymerase chain reaction (PCR) products were closely related to non-pylori helicobacters. Conclusion. Evidence for non-pylori helicobacters was scant in Finnish patients with inflammatory bowel disease or chronic but not end stage liver disease. We cannot, however, rule out their role in these diseases. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/969333
- author
- Veijola, Lea ; Nilsson, Ingrid LU ; Halme, Leena ; Abu Al-Soud, Waleed LU ; Makinen, Judit ; Ljungh, Åsa LU and Rautelin, Hilpi
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- blotting, Western, PCR, liver disease, Helicobacter, inflammatory bowel disease
- in
- Annals of Medicine
- volume
- 39
- issue
- 7
- pages
- 554 - 560
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000251046500006
- scopus:35648975290
- pmid:17852032
- ISSN
- 1365-2060
- DOI
- 10.1080/07853890701545714
- language
- English
- LU publication?
- yes
- id
- aeed408c-54a7-4fae-991d-ebc1dafbe5b5 (old id 969333)
- date added to LUP
- 2016-04-01 11:35:05
- date last changed
- 2022-01-26 07:20:37
@article{aeed408c-54a7-4fae-991d-ebc1dafbe5b5, abstract = {{Aim. To study the association between helicobacters and chronic liver diseases and chronic inflammatory bowel diseases. Patients and methods. Thirty-two patients with various chronic liver diseases and 137 patients with inflammatory bowel disease were enrolled. Antibodies to H. pylori, H. hepaticus, H. bilis, and H. pullorum were measured by enzyme immunoassay (EIA), and sera positive in a non-pylori helicobacter EIA were further examined by immunoblot assay. Detection of Helicobacter DNA in liver biopsies was done by denaturating gradient gel electrophoresis of PCR products (PCR-DGGE) and DNA sequence analysis. Results. Six inflammatory bowel disease patients, four with ulcerative colitis and two with Crohn's disease, and one liver disease patient with autoimmune cholangitis had antibodies to non-pylori helicobacters by an immunoblot assay. Four immunoblot assay-negative patients, three with autoimmune and one with non-autoimmune liver disease, had Helicobacter DNA in liver biopsies; three of the polymerase chain reaction (PCR) products were closely related to non-pylori helicobacters. Conclusion. Evidence for non-pylori helicobacters was scant in Finnish patients with inflammatory bowel disease or chronic but not end stage liver disease. We cannot, however, rule out their role in these diseases.}}, author = {{Veijola, Lea and Nilsson, Ingrid and Halme, Leena and Abu Al-Soud, Waleed and Makinen, Judit and Ljungh, Åsa and Rautelin, Hilpi}}, issn = {{1365-2060}}, keywords = {{blotting; Western; PCR; liver disease; Helicobacter; inflammatory bowel disease}}, language = {{eng}}, number = {{7}}, pages = {{554--560}}, publisher = {{Taylor & Francis}}, series = {{Annals of Medicine}}, title = {{Detection of Helicobacter species in chronic liver disease and chronic inflammatory bowel disease}}, url = {{http://dx.doi.org/10.1080/07853890701545714}}, doi = {{10.1080/07853890701545714}}, volume = {{39}}, year = {{2007}}, }