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Detection of Helicobacter species in chronic liver disease and chronic inflammatory bowel disease

Veijola, Lea; Nilsson, Ingrid LU ; Halme, Leena; Abu Al-Soud, Waleed LU ; Makinen, Judit; Ljungh, Åsa LU and Rautelin, Hilpi (2007) In Annals of Medicine 39(7). p.554-560
Abstract
Aim. To study the association between helicobacters and chronic liver diseases and chronic inflammatory bowel diseases. Patients and methods. Thirty-two patients with various chronic liver diseases and 137 patients with inflammatory bowel disease were enrolled. Antibodies to H. pylori, H. hepaticus, H. bilis, and H. pullorum were measured by enzyme immunoassay (EIA), and sera positive in a non-pylori helicobacter EIA were further examined by immunoblot assay. Detection of Helicobacter DNA in liver biopsies was done by denaturating gradient gel electrophoresis of PCR products (PCR-DGGE) and DNA sequence analysis. Results. Six inflammatory bowel disease patients, four with ulcerative colitis and two with Crohn's disease, and one liver... (More)
Aim. To study the association between helicobacters and chronic liver diseases and chronic inflammatory bowel diseases. Patients and methods. Thirty-two patients with various chronic liver diseases and 137 patients with inflammatory bowel disease were enrolled. Antibodies to H. pylori, H. hepaticus, H. bilis, and H. pullorum were measured by enzyme immunoassay (EIA), and sera positive in a non-pylori helicobacter EIA were further examined by immunoblot assay. Detection of Helicobacter DNA in liver biopsies was done by denaturating gradient gel electrophoresis of PCR products (PCR-DGGE) and DNA sequence analysis. Results. Six inflammatory bowel disease patients, four with ulcerative colitis and two with Crohn's disease, and one liver disease patient with autoimmune cholangitis had antibodies to non-pylori helicobacters by an immunoblot assay. Four immunoblot assay-negative patients, three with autoimmune and one with non-autoimmune liver disease, had Helicobacter DNA in liver biopsies; three of the polymerase chain reaction (PCR) products were closely related to non-pylori helicobacters. Conclusion. Evidence for non-pylori helicobacters was scant in Finnish patients with inflammatory bowel disease or chronic but not end stage liver disease. We cannot, however, rule out their role in these diseases. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
blotting, Western, PCR, liver disease, Helicobacter, inflammatory bowel disease
in
Annals of Medicine
volume
39
issue
7
pages
554 - 560
publisher
Informa Healthcare
external identifiers
  • wos:000251046500006
  • scopus:35648975290
ISSN
1365-2060
DOI
10.1080/07853890701545714
language
English
LU publication?
yes
id
aeed408c-54a7-4fae-991d-ebc1dafbe5b5 (old id 969333)
date added to LUP
2008-01-30 09:45:09
date last changed
2017-02-19 03:24:17
@article{aeed408c-54a7-4fae-991d-ebc1dafbe5b5,
  abstract     = {Aim. To study the association between helicobacters and chronic liver diseases and chronic inflammatory bowel diseases. Patients and methods. Thirty-two patients with various chronic liver diseases and 137 patients with inflammatory bowel disease were enrolled. Antibodies to H. pylori, H. hepaticus, H. bilis, and H. pullorum were measured by enzyme immunoassay (EIA), and sera positive in a non-pylori helicobacter EIA were further examined by immunoblot assay. Detection of Helicobacter DNA in liver biopsies was done by denaturating gradient gel electrophoresis of PCR products (PCR-DGGE) and DNA sequence analysis. Results. Six inflammatory bowel disease patients, four with ulcerative colitis and two with Crohn's disease, and one liver disease patient with autoimmune cholangitis had antibodies to non-pylori helicobacters by an immunoblot assay. Four immunoblot assay-negative patients, three with autoimmune and one with non-autoimmune liver disease, had Helicobacter DNA in liver biopsies; three of the polymerase chain reaction (PCR) products were closely related to non-pylori helicobacters. Conclusion. Evidence for non-pylori helicobacters was scant in Finnish patients with inflammatory bowel disease or chronic but not end stage liver disease. We cannot, however, rule out their role in these diseases.},
  author       = {Veijola, Lea and Nilsson, Ingrid and Halme, Leena and Abu Al-Soud, Waleed and Makinen, Judit and Ljungh, Åsa and Rautelin, Hilpi},
  issn         = {1365-2060},
  keyword      = {blotting,Western,PCR,liver disease,Helicobacter,inflammatory bowel disease},
  language     = {eng},
  number       = {7},
  pages        = {554--560},
  publisher    = {Informa Healthcare},
  series       = {Annals of Medicine},
  title        = {Detection of Helicobacter species in chronic liver disease and chronic inflammatory bowel disease},
  url          = {http://dx.doi.org/10.1080/07853890701545714},
  volume       = {39},
  year         = {2007},
}