Comparison of NCL-hTERT antibody reactivity and telomere repeat amplification protocol in situ in effusions
(2007) In Acta Cytologica 51(6). p.886-892- Abstract
- Objective To compare the performances of 2 methods, telomerase repeat amplification protocol (TRAP) in situ and antibodies to the hTERT protein, in assessing telomerase activity. Study Design TRAP in situ and immunomercial antibody (NCL-hTERT) was performed on 54 body cavity effusions. The results were compared and correlated to diagnosis. Results Thirty-four effitsions from patients with verified malignant disease contained cytologically malignant cells. Both methods were positive in 33 of the cases, whereas only hTERT was positive in I case. Twenty effusions, all containing mesothelial cells, came from patients with benign conditions. In 2 fluids atypical, hyperplastic mesothelial cells were both TRAP in situ and hTERT positive. All... (More)
- Objective To compare the performances of 2 methods, telomerase repeat amplification protocol (TRAP) in situ and antibodies to the hTERT protein, in assessing telomerase activity. Study Design TRAP in situ and immunomercial antibody (NCL-hTERT) was performed on 54 body cavity effusions. The results were compared and correlated to diagnosis. Results Thirty-four effitsions from patients with verified malignant disease contained cytologically malignant cells. Both methods were positive in 33 of the cases, whereas only hTERT was positive in I case. Twenty effusions, all containing mesothelial cells, came from patients with benign conditions. In 2 fluids atypical, hyperplastic mesothelial cells were both TRAP in situ and hTERT positive. All remaining 18 fluids were TRAP in situ negative, whereas 12 of 18 were hTERT positive. Thus the results of TRAP in situ and hTERT immunohistochemistry disagreed in 1 of 34 (3%) malignant and 12 of 20 (60%) benign cases. Conclusion The sensitivities for malignancy were similar for TRAP in situ and hTERT immunobistochemistry. The specificity of the applied hTERT antibody was significantly lower, due to hTERT reactivity in mesothelial cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/969345
- author
- Zendehrokh, Nooreldin LU ; Rehnberg, Johanna and Dejmek, Annika LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- nucleolin, mesothelial cells, malignancy, immunohistochemistry, effusion, hTERT, telomere repeat amplification protocol, telomerase
- in
- Acta Cytologica
- volume
- 51
- issue
- 6
- pages
- 886 - 892
- publisher
- Karger
- external identifiers
-
- wos:000251045800009
- pmid:18077981
- scopus:36348939182
- ISSN
- 0001-5547
- language
- English
- LU publication?
- yes
- id
- 6c7487cf-7b83-4e42-8812-087bd8fd122c (old id 969345)
- alternative location
- http://www.acta-cytol.com/toc/auto_abstract.php?id=22852
- http://www.ncbi.nlm.nih.gov/pubmed/18077981?dopt=Abstract
- date added to LUP
- 2016-04-01 15:34:07
- date last changed
- 2022-01-28 06:00:00
@article{6c7487cf-7b83-4e42-8812-087bd8fd122c,
abstract = {{Objective To compare the performances of 2 methods, telomerase repeat amplification protocol (TRAP) in situ and antibodies to the hTERT protein, in assessing telomerase activity. Study Design TRAP in situ and immunomercial antibody (NCL-hTERT) was performed on 54 body cavity effusions. The results were compared and correlated to diagnosis. Results Thirty-four effitsions from patients with verified malignant disease contained cytologically malignant cells. Both methods were positive in 33 of the cases, whereas only hTERT was positive in I case. Twenty effusions, all containing mesothelial cells, came from patients with benign conditions. In 2 fluids atypical, hyperplastic mesothelial cells were both TRAP in situ and hTERT positive. All remaining 18 fluids were TRAP in situ negative, whereas 12 of 18 were hTERT positive. Thus the results of TRAP in situ and hTERT immunohistochemistry disagreed in 1 of 34 (3%) malignant and 12 of 20 (60%) benign cases. Conclusion The sensitivities for malignancy were similar for TRAP in situ and hTERT immunobistochemistry. The specificity of the applied hTERT antibody was significantly lower, due to hTERT reactivity in mesothelial cells.}},
author = {{Zendehrokh, Nooreldin and Rehnberg, Johanna and Dejmek, Annika}},
issn = {{0001-5547}},
keywords = {{nucleolin; mesothelial cells; malignancy; immunohistochemistry; effusion; hTERT; telomere repeat amplification protocol; telomerase}},
language = {{eng}},
number = {{6}},
pages = {{886--892}},
publisher = {{Karger}},
series = {{Acta Cytologica}},
title = {{Comparison of NCL-hTERT antibody reactivity and telomere repeat amplification protocol in situ in effusions}},
url = {{http://www.acta-cytol.com/toc/auto_abstract.php?id=22852}},
volume = {{51}},
year = {{2007}},
}