Functionally associated targets in mantle cell lymphoma as defined by DNA microarrays and RNA interference
(2008) In Blood 111(3). p.1617-1624- Abstract
- Mantle cell lymphoma (MCL) is a non-Hodgkin lymphoma with poor prognosis. Its hallmark is the translocation t(11:14)q (13;32), leading to overexpression of cyclin D1, a positive regulator of the cell cycle. As cyclin D1 up-regulation is not sufficient for inducing malignant transformation, we combined DNA microarray and RNA interference (RNAi) approaches to identify novel deregulated genes involved in the progression of MCL. DNA microarray analysis identified 46 genes specifically up-regulated in MCL compared with normal B cells; 20 of these were chosen for further studies based on their cellular functions, such as growth and proliferation. The Granta 519 cell line was selected as an MCL in vitro model, to set up the RNAi protocol. To... (More)
- Mantle cell lymphoma (MCL) is a non-Hodgkin lymphoma with poor prognosis. Its hallmark is the translocation t(11:14)q (13;32), leading to overexpression of cyclin D1, a positive regulator of the cell cycle. As cyclin D1 up-regulation is not sufficient for inducing malignant transformation, we combined DNA microarray and RNA interference (RNAi) approaches to identify novel deregulated genes involved in the progression of MCL. DNA microarray analysis identified 46 genes specifically up-regulated in MCL compared with normal B cells; 20 of these were chosen for further studies based on their cellular functions, such as growth and proliferation. The Granta 519 cell line was selected as an MCL in vitro model, to set up the RNAi protocol. To confirm the functionality of overexpression of the 20 disease-associated genes, they were knocked down using small interfering RNAs (siRNAs). In particular, knockdown of 3 genes, encoding the hepatoma-derived growth factor related protein 3 (HDGFRP3), the frizzled homolog 2 (FZD2), and the dual specificity phosphatase 5 (DUSP5), induced proliferative arrest in Granta 519 MCL cells. These genes emerged as functionally associated in MCL, in relation to growth and survival, and interfering with their function would increase insight into lymphoma growth regulation, potentially leading to novel clinical intervention modalities. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1042574
- author
- Ortega-Paino, Eva LU ; Fransson, Johan LU ; Ek, Sara LU and Borrebaeck, Carl LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Blood
- volume
- 111
- issue
- 3
- pages
- 1617 - 1624
- publisher
- American Society of Hematology
- external identifiers
-
- wos:000252792900094
- scopus:38949205610
- pmid:18024791
- ISSN
- 1528-0020
- DOI
- 10.1182/blood-2007-02-068791
- language
- English
- LU publication?
- yes
- id
- 9699dce2-4f8a-4997-988a-8a3014fc742c (old id 1042574)
- date added to LUP
- 2016-04-01 12:30:18
- date last changed
- 2022-01-27 05:59:48
@article{9699dce2-4f8a-4997-988a-8a3014fc742c,
abstract = {{Mantle cell lymphoma (MCL) is a non-Hodgkin lymphoma with poor prognosis. Its hallmark is the translocation t(11:14)q (13;32), leading to overexpression of cyclin D1, a positive regulator of the cell cycle. As cyclin D1 up-regulation is not sufficient for inducing malignant transformation, we combined DNA microarray and RNA interference (RNAi) approaches to identify novel deregulated genes involved in the progression of MCL. DNA microarray analysis identified 46 genes specifically up-regulated in MCL compared with normal B cells; 20 of these were chosen for further studies based on their cellular functions, such as growth and proliferation. The Granta 519 cell line was selected as an MCL in vitro model, to set up the RNAi protocol. To confirm the functionality of overexpression of the 20 disease-associated genes, they were knocked down using small interfering RNAs (siRNAs). In particular, knockdown of 3 genes, encoding the hepatoma-derived growth factor related protein 3 (HDGFRP3), the frizzled homolog 2 (FZD2), and the dual specificity phosphatase 5 (DUSP5), induced proliferative arrest in Granta 519 MCL cells. These genes emerged as functionally associated in MCL, in relation to growth and survival, and interfering with their function would increase insight into lymphoma growth regulation, potentially leading to novel clinical intervention modalities.}},
author = {{Ortega-Paino, Eva and Fransson, Johan and Ek, Sara and Borrebaeck, Carl}},
issn = {{1528-0020}},
language = {{eng}},
number = {{3}},
pages = {{1617--1624}},
publisher = {{American Society of Hematology}},
series = {{Blood}},
title = {{Functionally associated targets in mantle cell lymphoma as defined by DNA microarrays and RNA interference}},
url = {{http://dx.doi.org/10.1182/blood-2007-02-068791}},
doi = {{10.1182/blood-2007-02-068791}},
volume = {{111}},
year = {{2008}},
}