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Oxidation-reduction potential (ORP) as a tool for process monitoring of H2O2/LPMO assisted enzymatic hydrolysis of cellulose

Kadić, A. LU ; Chylenski, P.; Tengstedt Hansen, Mads Anders; Bengtsson, O.; Eijsink, Vincent G.H. and Lidén, Gunnar LU (2019) In Process Biochemistry
Abstract

Oxidation-reduction potential (ORP) is an environmental factor of importance in several biological conversion processes. Lytic polysaccharide monooxygenases (LPMOs) catalyze oxidative disruption of the cellulose chain in the presence of oxygen or hydrogen peroxide and increase enzymatic hydrolysis yields. However, the enzymes are also sensitive to oxidative damage and the level of oxidative agent needs to be controlled to avoid inactivation of the LPMOs. In the current study, enzymatic hydrolysis of sulfite-pretreated softwood (12% DM loading) was carried out in lab scale reactors with gradual addition of hydrogen peroxide using an LPMO-containing commercial enzyme cocktail. The ORP was measured during enzymatic hydrolysis together with... (More)

Oxidation-reduction potential (ORP) is an environmental factor of importance in several biological conversion processes. Lytic polysaccharide monooxygenases (LPMOs) catalyze oxidative disruption of the cellulose chain in the presence of oxygen or hydrogen peroxide and increase enzymatic hydrolysis yields. However, the enzymes are also sensitive to oxidative damage and the level of oxidative agent needs to be controlled to avoid inactivation of the LPMOs. In the current study, enzymatic hydrolysis of sulfite-pretreated softwood (12% DM loading) was carried out in lab scale reactors with gradual addition of hydrogen peroxide using an LPMO-containing commercial enzyme cocktail. The ORP was measured during enzymatic hydrolysis together with released glucose and the level of C4-oxidized dimer as a marker for LPMO activity. Arrests in LPMO activity coincided with a markedly changed ORP signal and this was utilized in subsequent experiments in which the feed rate of hydrogen peroxide was controlled by keeping the ORP at predetermined set-points of -40 mV, -80 mV and -120 mV. Under anaerobic conditions, the highest hydrolysis yield (78% after 72 h) was found for the ORP set-point of -80 mV. The results show that ORP can serve as an indicator of LPMO activity and may help optimizing overall process efficiency.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Cellulose, Enzymatic hydrolysis, Hydrogen peroxide, Lytic polysaccharide monooxygenase (LPMO), Oxidation-reduction potential (ORP), Redox
in
Process Biochemistry
publisher
Elsevier
external identifiers
  • scopus:85070876026
ISSN
1359-5113
DOI
10.1016/j.procbio.2019.08.015
language
English
LU publication?
yes
id
96b041c0-3c09-4f6c-8963-b6309a98e9fd
date added to LUP
2019-09-09 10:35:30
date last changed
2019-09-26 04:41:49
@article{96b041c0-3c09-4f6c-8963-b6309a98e9fd,
  abstract     = {<p>Oxidation-reduction potential (ORP) is an environmental factor of importance in several biological conversion processes. Lytic polysaccharide monooxygenases (LPMOs) catalyze oxidative disruption of the cellulose chain in the presence of oxygen or hydrogen peroxide and increase enzymatic hydrolysis yields. However, the enzymes are also sensitive to oxidative damage and the level of oxidative agent needs to be controlled to avoid inactivation of the LPMOs. In the current study, enzymatic hydrolysis of sulfite-pretreated softwood (12% DM loading) was carried out in lab scale reactors with gradual addition of hydrogen peroxide using an LPMO-containing commercial enzyme cocktail. The ORP was measured during enzymatic hydrolysis together with released glucose and the level of C4-oxidized dimer as a marker for LPMO activity. Arrests in LPMO activity coincided with a markedly changed ORP signal and this was utilized in subsequent experiments in which the feed rate of hydrogen peroxide was controlled by keeping the ORP at predetermined set-points of -40 mV, -80 mV and -120 mV. Under anaerobic conditions, the highest hydrolysis yield (78% after 72 h) was found for the ORP set-point of -80 mV. The results show that ORP can serve as an indicator of LPMO activity and may help optimizing overall process efficiency.</p>},
  author       = {Kadić, A. and Chylenski, P. and Tengstedt Hansen, Mads Anders and Bengtsson, O. and Eijsink, Vincent G.H. and Lidén, Gunnar},
  issn         = {1359-5113},
  keyword      = {Cellulose,Enzymatic hydrolysis,Hydrogen peroxide,Lytic polysaccharide monooxygenase (LPMO),Oxidation-reduction potential (ORP),Redox},
  language     = {eng},
  publisher    = {Elsevier},
  series       = {Process Biochemistry},
  title        = {Oxidation-reduction potential (ORP) as a tool for process monitoring of H<sub>2</sub>O<sub>2</sub>/LPMO assisted enzymatic hydrolysis of cellulose},
  url          = {http://dx.doi.org/10.1016/j.procbio.2019.08.015},
  year         = {2019},
}