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Reporting on methods to generate and purify rodent and human oligodendrocytes from different sources

Djelloul, Mehdi LU ; Azevedo, Carla LU ; Pomeshchik, Yuriy LU ; Hammarberg, Anna LU and Roybon, Laurent LU (2017) In Stem Cell Research 20. p.58-66
Abstract

Oligodendrocytes are part of the glial cells located in the central nervous system, capable of providing trophic support to neurons and ensheathing their axons. These cells can become dysfunctional under pathologic condition. Rodent and human pluripotent stem cells are inexhaustible sources for producing oligodendrocytes that can be used for disease modeling and cell replacement therapy studies. They also offer many opportunities to model the contribution of oligodendrocytes in non-genetic disorders such as multiple system atrophy. In this method article, we provide robust and reproducible differentiation protocols to obtain oligodendrocyte progenitor cells and purify them using fluorescence activated cell sorting.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
FACS, O4, Oligodendrocytes, OPC purification, PDGFRα/CD140, Pluripotent stem cells
in
Stem Cell Research
volume
20
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:85014356330
  • wos:000402474700011
ISSN
1873-5061
DOI
10.1016/j.scr.2017.02.008
language
English
LU publication?
yes
id
96c78c54-c6b6-42ee-b3a6-594e937656ca
date added to LUP
2017-03-16 12:03:42
date last changed
2018-01-07 11:55:52
@article{96c78c54-c6b6-42ee-b3a6-594e937656ca,
  abstract     = {<p>Oligodendrocytes are part of the glial cells located in the central nervous system, capable of providing trophic support to neurons and ensheathing their axons. These cells can become dysfunctional under pathologic condition. Rodent and human pluripotent stem cells are inexhaustible sources for producing oligodendrocytes that can be used for disease modeling and cell replacement therapy studies. They also offer many opportunities to model the contribution of oligodendrocytes in non-genetic disorders such as multiple system atrophy. In this method article, we provide robust and reproducible differentiation protocols to obtain oligodendrocyte progenitor cells and purify them using fluorescence activated cell sorting.</p>},
  author       = {Djelloul, Mehdi and Azevedo, Carla and Pomeshchik, Yuriy and Hammarberg, Anna and Roybon, Laurent},
  issn         = {1873-5061},
  keyword      = {FACS,O4,Oligodendrocytes,OPC purification,PDGFRα/CD140,Pluripotent stem cells},
  language     = {eng},
  month        = {04},
  pages        = {58--66},
  publisher    = {Elsevier},
  series       = {Stem Cell Research},
  title        = {Reporting on methods to generate and purify rodent and human oligodendrocytes from different sources},
  url          = {http://dx.doi.org/10.1016/j.scr.2017.02.008},
  volume       = {20},
  year         = {2017},
}