Reporting on methods to generate and purify rodent and human oligodendrocytes from different sources
(2017) In Stem Cell Research 20. p.58-66- Abstract
Oligodendrocytes are part of the glial cells located in the central nervous system, capable of providing trophic support to neurons and ensheathing their axons. These cells can become dysfunctional under pathologic condition. Rodent and human pluripotent stem cells are inexhaustible sources for producing oligodendrocytes that can be used for disease modeling and cell replacement therapy studies. They also offer many opportunities to model the contribution of oligodendrocytes in non-genetic disorders such as multiple system atrophy. In this method article, we provide robust and reproducible differentiation protocols to obtain oligodendrocyte progenitor cells and purify them using fluorescence activated cell sorting.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/96c78c54-c6b6-42ee-b3a6-594e937656ca
- author
- Djelloul, Mehdi
LU
; Azevedo, Carla
LU
; Pomeshchik, Yuriy
LU
; Hammarberg, Anna LU and Roybon, Laurent LU
- organization
- publishing date
- 2017-04-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- FACS, O4, Oligodendrocytes, OPC purification, PDGFRα/CD140, Pluripotent stem cells
- in
- Stem Cell Research
- volume
- 20
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85014356330
- pmid:28395742
- wos:000402474700011
- ISSN
- 1873-5061
- DOI
- 10.1016/j.scr.2017.02.008
- language
- English
- LU publication?
- yes
- id
- 96c78c54-c6b6-42ee-b3a6-594e937656ca
- date added to LUP
- 2017-03-16 12:03:42
- date last changed
- 2025-01-07 09:53:03
@article{96c78c54-c6b6-42ee-b3a6-594e937656ca, abstract = {{<p>Oligodendrocytes are part of the glial cells located in the central nervous system, capable of providing trophic support to neurons and ensheathing their axons. These cells can become dysfunctional under pathologic condition. Rodent and human pluripotent stem cells are inexhaustible sources for producing oligodendrocytes that can be used for disease modeling and cell replacement therapy studies. They also offer many opportunities to model the contribution of oligodendrocytes in non-genetic disorders such as multiple system atrophy. In this method article, we provide robust and reproducible differentiation protocols to obtain oligodendrocyte progenitor cells and purify them using fluorescence activated cell sorting.</p>}}, author = {{Djelloul, Mehdi and Azevedo, Carla and Pomeshchik, Yuriy and Hammarberg, Anna and Roybon, Laurent}}, issn = {{1873-5061}}, keywords = {{FACS; O4; Oligodendrocytes; OPC purification; PDGFRα/CD140; Pluripotent stem cells}}, language = {{eng}}, month = {{04}}, pages = {{58--66}}, publisher = {{Elsevier}}, series = {{Stem Cell Research}}, title = {{Reporting on methods to generate and purify rodent and human oligodendrocytes from different sources}}, url = {{http://dx.doi.org/10.1016/j.scr.2017.02.008}}, doi = {{10.1016/j.scr.2017.02.008}}, volume = {{20}}, year = {{2017}}, }