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Compositional differences between infant and adult human corneal basement membranes

Kabosova, Andrea; Azar, Dimitri T.; Bannikov, Gregory A.; Campbell, Kevin P.; Durbeej-Hjalt, Madeleine LU ; Ghohestani, Reza F.; Jones, Jonathan C. R.; Kenney, M. Cristina; Koch, Manuel and Ninomiya, Yoshifumi, et al. (2007) In Investigative Ophthalmology & Visual Science 48(11). p.4989-4999
Abstract
PURPOSE. Adult human corneal epithelial basement membrane ( EBM) and Descemet's membrane ( DM) components exhibit heterogeneous distribution. The purpose of the study was to identify changes of these components during postnatal corneal development. METHODS. Thirty healthy adult corneas and 10 corneas from 12-day- to 3-year-old children were studied by immunofluorescence with antibodies against BM components. RESULTS. Type IV collagen composition of infant corneal central EBM over Bowman's layer changed from alpha 1-alpha 2 to alpha 3-alpha 4 chains after 3 years of life; in the adult, alpha 1-alpha 2 chains were retained only in the limbal BM. Laminin alpha 2 and beta 2 chains were present in the adult limbal BM where epithelial stem cells... (More)
PURPOSE. Adult human corneal epithelial basement membrane ( EBM) and Descemet's membrane ( DM) components exhibit heterogeneous distribution. The purpose of the study was to identify changes of these components during postnatal corneal development. METHODS. Thirty healthy adult corneas and 10 corneas from 12-day- to 3-year-old children were studied by immunofluorescence with antibodies against BM components. RESULTS. Type IV collagen composition of infant corneal central EBM over Bowman's layer changed from alpha 1-alpha 2 to alpha 3-alpha 4 chains after 3 years of life; in the adult, alpha 1-alpha 2 chains were retained only in the limbal BM. Laminin alpha 2 and beta 2 chains were present in the adult limbal BM where epithelial stem cells are located. By 3 years of age, beta 2 chain appeared in the limbal BM. In all corneas, limbal BM contained laminin gamma 3 chain. In the infant DM, type IV collagen alpha 1-alpha 6 chains, perlecan, nidogen-1, nidogen-2, and netrin-4 were found on both faces, but they remained only on the endothelial face of the adult DM. The stromal face of the infant but not the adult DM was positive for tenascin-C, fibrillin-1, SPARC, and laminin-332. Type VIII collagen shifted from the endothelial face of infant DM to its stromal face in the adult. Matrilin-4 largely disappeared after the age of 3 years. CONCLUSIONS. The distribution of laminin gamma 3 chain, nidogen-2, netrin-4, matrilin-2, and matrilin-4 is described in the cornea for the first time. The observed differences between adult and infant corneal BMs may relate to changes in their mechanical strength, corneal cell adhesion and differentiation in the process of postnatal corneal maturation. (Less)
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publication status
published
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Investigative Ophthalmology & Visual Science
volume
48
issue
11
pages
4989 - 4999
publisher
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
external identifiers
  • wos:000250734800020
  • scopus:38449103731
ISSN
1552-5783
DOI
10.1167/iovs.07-0654
language
English
LU publication?
yes
id
d91cece3-9e9a-40d8-8a11-59f9c408ec98 (old id 971895)
date added to LUP
2008-01-29 14:43:01
date last changed
2017-10-01 04:49:13
@article{d91cece3-9e9a-40d8-8a11-59f9c408ec98,
  abstract     = {PURPOSE. Adult human corneal epithelial basement membrane ( EBM) and Descemet's membrane ( DM) components exhibit heterogeneous distribution. The purpose of the study was to identify changes of these components during postnatal corneal development. METHODS. Thirty healthy adult corneas and 10 corneas from 12-day- to 3-year-old children were studied by immunofluorescence with antibodies against BM components. RESULTS. Type IV collagen composition of infant corneal central EBM over Bowman's layer changed from alpha 1-alpha 2 to alpha 3-alpha 4 chains after 3 years of life; in the adult, alpha 1-alpha 2 chains were retained only in the limbal BM. Laminin alpha 2 and beta 2 chains were present in the adult limbal BM where epithelial stem cells are located. By 3 years of age, beta 2 chain appeared in the limbal BM. In all corneas, limbal BM contained laminin gamma 3 chain. In the infant DM, type IV collagen alpha 1-alpha 6 chains, perlecan, nidogen-1, nidogen-2, and netrin-4 were found on both faces, but they remained only on the endothelial face of the adult DM. The stromal face of the infant but not the adult DM was positive for tenascin-C, fibrillin-1, SPARC, and laminin-332. Type VIII collagen shifted from the endothelial face of infant DM to its stromal face in the adult. Matrilin-4 largely disappeared after the age of 3 years. CONCLUSIONS. The distribution of laminin gamma 3 chain, nidogen-2, netrin-4, matrilin-2, and matrilin-4 is described in the cornea for the first time. The observed differences between adult and infant corneal BMs may relate to changes in their mechanical strength, corneal cell adhesion and differentiation in the process of postnatal corneal maturation.},
  author       = {Kabosova, Andrea and Azar, Dimitri T. and Bannikov, Gregory A. and Campbell, Kevin P. and Durbeej-Hjalt, Madeleine and Ghohestani, Reza F. and Jones, Jonathan C. R. and Kenney, M. Cristina and Koch, Manuel and Ninomiya, Yoshifumi and Patton, Bruce L. and Paulsson, Mats and Sado, Yoshikazu and Sage, E. Helene and Sasaki, Takako and Sorokin, Lydia M. and Steiner-Champliaud, Marie-France and Sun, Tung-Tien and SundarRaj, Nirmala and Timpl, Rupert and Virtanen, Ismo and Ljubimov, Alexander V.},
  issn         = {1552-5783},
  language     = {eng},
  number       = {11},
  pages        = {4989--4999},
  publisher    = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
  series       = {Investigative Ophthalmology & Visual Science},
  title        = {Compositional differences between infant and adult human corneal basement membranes},
  url          = {http://dx.doi.org/10.1167/iovs.07-0654},
  volume       = {48},
  year         = {2007},
}