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Improvement of retroviral retargeting by using amino acid spacers between an additional binding domain and the N terminus of Moloney murine leukemia virus SU

Valsesia-Wittmann, S; Morling, F J; Nilson, B H LU ; Takeuchi, Y; Russell, S J and Cosset, F L (1996) In Journal of Virology 70(3). p.2059-2064
Abstract

We previously reported a strategy to redirect the retroviral host range by expressing single-chain antibodies (S. J. Russell, R. E. Hawkins, and G. Winter, Nucleic Acids Res. 21:1081-1085, 1993) or ligands (F.-L. Cosset, F. J Morling, Y. Takeuchi, R. A. Weiss, M. K. L. Collins, and S. J. Russell, J. Virol. 69:6314-6322, 1995) at the N terminus of Moloney murine leukemia virus (MoMLV) surface proteins (SU). Although such chimeric envelopes were able to bind the new receptors, the transduction efficiency of retargeted viruses was generally low. We hypothesized that conformational rearrangements of envelope glycoproteins were not optimally triggered following binding, and to overcome these postbinding blocks, we have generated here a set... (More)

We previously reported a strategy to redirect the retroviral host range by expressing single-chain antibodies (S. J. Russell, R. E. Hawkins, and G. Winter, Nucleic Acids Res. 21:1081-1085, 1993) or ligands (F.-L. Cosset, F. J Morling, Y. Takeuchi, R. A. Weiss, M. K. L. Collins, and S. J. Russell, J. Virol. 69:6314-6322, 1995) at the N terminus of Moloney murine leukemia virus (MoMLV) surface proteins (SU). Although such chimeric envelopes were able to bind the new receptors, the transduction efficiency of retargeted viruses was generally low. We hypothesized that conformational rearrangements of envelope glycoproteins were not optimally triggered following binding, and to overcome these postbinding blocks, we have generated here a set of chimeric MoMLV-derived envelopes targeted to the Ram-1 phosphate transporter in which we have varied the spacing between the Ram-1-binding domain and the MoMLV SU. All of the recombinant envelopes were correctly expressed on virions, and all bound efficiently to Ram-1. However, the interdomain spacing greatly affected the efficiency of gene transfer by retroviral vectors that had bound to Ram-1 via their chimeric envelopes. Optimal interdomain spacing allowed a 100-fold-increased viral transduction via Ram-1 compared to our previous results.

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keywords
3T3 Cells, Animals, Base Sequence, Cell Line, Cloning, Molecular, DNA, Viral, Humans, Mice, Molecular Sequence Data, Moloney murine leukemia virus, Mutagenesis, Phosphate Transport Proteins, Receptors, Virus, Recombinant Fusion Proteins, Retroviridae Proteins, Oncogenic, Sodium-Phosphate Cotransporter Proteins, Sodium-Phosphate Cotransporter Proteins, Type III, Symporters, Transformation, Genetic, Viral Envelope Proteins, Journal Article, Research Support, Non-U.S. Gov't
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Journal of Virology
volume
70
issue
3
pages
6 pages
publisher
American Society for Microbiology
external identifiers
  • scopus:0030031348
ISSN
0022-538X
language
English
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no
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9731e390-b3a4-40d5-b323-6186a4f7530f
date added to LUP
2018-05-26 13:56:52
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2018-07-17 15:39:27
@article{9731e390-b3a4-40d5-b323-6186a4f7530f,
  abstract     = {<p>We previously reported a strategy to redirect the retroviral host range by expressing single-chain antibodies (S. J. Russell, R. E. Hawkins, and G. Winter, Nucleic Acids Res. 21:1081-1085, 1993) or ligands (F.-L. Cosset, F. J Morling, Y. Takeuchi, R. A. Weiss, M. K. L. Collins, and S. J. Russell, J. Virol. 69:6314-6322, 1995) at the N terminus of Moloney murine leukemia virus (MoMLV) surface proteins (SU). Although such chimeric envelopes were able to bind the new receptors, the transduction efficiency of retargeted viruses was generally low. We hypothesized that conformational rearrangements of envelope glycoproteins were not optimally triggered following binding, and to overcome these postbinding blocks, we have generated here a set of chimeric MoMLV-derived envelopes targeted to the Ram-1 phosphate transporter in which we have varied the spacing between the Ram-1-binding domain and the MoMLV SU. All of the recombinant envelopes were correctly expressed on virions, and all bound efficiently to Ram-1. However, the interdomain spacing greatly affected the efficiency of gene transfer by retroviral vectors that had bound to Ram-1 via their chimeric envelopes. Optimal interdomain spacing allowed a 100-fold-increased viral transduction via Ram-1 compared to our previous results.</p>},
  author       = {Valsesia-Wittmann, S and Morling, F J and Nilson, B H and Takeuchi, Y and Russell, S J and Cosset, F L},
  issn         = {0022-538X},
  keyword      = {3T3 Cells,Animals,Base Sequence,Cell Line,Cloning, Molecular,DNA, Viral,Humans,Mice,Molecular Sequence Data,Moloney murine leukemia virus,Mutagenesis,Phosphate Transport Proteins,Receptors, Virus,Recombinant Fusion Proteins,Retroviridae Proteins, Oncogenic,Sodium-Phosphate Cotransporter Proteins,Sodium-Phosphate Cotransporter Proteins, Type III,Symporters,Transformation, Genetic,Viral Envelope Proteins,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {2059--2064},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Virology},
  title        = {Improvement of retroviral retargeting by using amino acid spacers between an additional binding domain and the N terminus of Moloney murine leukemia virus SU},
  volume       = {70},
  year         = {1996},
}