Molecular characterization of coxsackievirus a24v from feces and conjunctiva reveals epidemiological links
(2021) In Microorganisms 9(3). p.1-10- Abstract
Coxsackievirus A24 variant (CVA24v), the main causative agent of acute hemorrhagic conjunctivitis (AHC), can be isolated from both the eyes and lower alimentary tract. However, the molecular features of CVA24v in feces is not well-documented. In this study, we compared the VP1 and 3C sequences of CVA24v strains isolated from feces during AHC epidemics in Cuba in 1997, 2003, and 2008–2009 with those obtained from conjunctival swabs during the same epidemic period. The sequence analyses of the 3C and VP1 region of stool isolates from the three epidemics showed a high degree of nucleotide identity (ranging from 97.3–100%) to the corresponding conjunctival isolates. The phylogenetic analysis showed that fecal CVA24v isolates from the 1997... (More)
Coxsackievirus A24 variant (CVA24v), the main causative agent of acute hemorrhagic conjunctivitis (AHC), can be isolated from both the eyes and lower alimentary tract. However, the molecular features of CVA24v in feces is not well-documented. In this study, we compared the VP1 and 3C sequences of CVA24v strains isolated from feces during AHC epidemics in Cuba in 1997, 2003, and 2008–2009 with those obtained from conjunctival swabs during the same epidemic period. The sequence analyses of the 3C and VP1 region of stool isolates from the three epidemics showed a high degree of nucleotide identity (ranging from 97.3–100%) to the corresponding conjunctival isolates. The phylogenetic analysis showed that fecal CVA24v isolates from the 1997 and 2003 Cuban outbreaks formed a clade with CVA24v strains isolated from conjunctival swabs in Cuba and other countries during the same period. There were three amino acid changes (3C region) and one amino acid change (VP1 region) in seven CVA24v strains isolated sequentially over 20 days from fecal samples of one patient, suggesting viral replication in the intestine. Despite these substitutions, the virus from the conjunctival swab and fecal samples were genetically very similar. Therefore, fecal samples should be considered as a reliable alternative sample type for the routine molecular diagnosis and molecular epidemiology of CVA24v, also during outbreaks of AHC.
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- author
- Fonseca, Magilé C. ; Pupo-Meriño, Mario ; García-González, Luis A. ; Muné, Mayra ; Resik, Sonia ; Norder, Heléne and Sarmiento, Luis LU
- organization
- publishing date
- 2021-03
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Acute hemorrhagic conjunctivitis, Conjunctival swabs, Coxsackievirus A24v, Enterovirus, Feces
- in
- Microorganisms
- volume
- 9
- issue
- 3
- article number
- 531
- pages
- 10 pages
- publisher
- MDPI AG
- external identifiers
-
- scopus:85101969088
- pmid:33807540
- ISSN
- 2076-2607
- DOI
- 10.3390/microorganisms9030531
- language
- English
- LU publication?
- yes
- additional info
- Funding Information: Funding: This study was partially supported by Wood–Whelan Research Fellowships 2007 from the International Union of Biochemistry and Molecular Biology (IUBMB). © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
- id
- 9769f71e-bafb-43fe-accb-01020edd6305
- date added to LUP
- 2021-03-26 16:57:16
- date last changed
- 2024-06-15 08:36:38
@article{9769f71e-bafb-43fe-accb-01020edd6305,
abstract = {{<p>Coxsackievirus A24 variant (CVA24v), the main causative agent of acute hemorrhagic conjunctivitis (AHC), can be isolated from both the eyes and lower alimentary tract. However, the molecular features of CVA24v in feces is not well-documented. In this study, we compared the VP1 and 3C sequences of CVA24v strains isolated from feces during AHC epidemics in Cuba in 1997, 2003, and 2008–2009 with those obtained from conjunctival swabs during the same epidemic period. The sequence analyses of the 3C and VP1 region of stool isolates from the three epidemics showed a high degree of nucleotide identity (ranging from 97.3–100%) to the corresponding conjunctival isolates. The phylogenetic analysis showed that fecal CVA24v isolates from the 1997 and 2003 Cuban outbreaks formed a clade with CVA24v strains isolated from conjunctival swabs in Cuba and other countries during the same period. There were three amino acid changes (3C region) and one amino acid change (VP1 region) in seven CVA24v strains isolated sequentially over 20 days from fecal samples of one patient, suggesting viral replication in the intestine. Despite these substitutions, the virus from the conjunctival swab and fecal samples were genetically very similar. Therefore, fecal samples should be considered as a reliable alternative sample type for the routine molecular diagnosis and molecular epidemiology of CVA24v, also during outbreaks of AHC.</p>}},
author = {{Fonseca, Magilé C. and Pupo-Meriño, Mario and García-González, Luis A. and Muné, Mayra and Resik, Sonia and Norder, Heléne and Sarmiento, Luis}},
issn = {{2076-2607}},
keywords = {{Acute hemorrhagic conjunctivitis; Conjunctival swabs; Coxsackievirus A24v; Enterovirus; Feces}},
language = {{eng}},
number = {{3}},
pages = {{1--10}},
publisher = {{MDPI AG}},
series = {{Microorganisms}},
title = {{Molecular characterization of coxsackievirus a24v from feces and conjunctiva reveals epidemiological links}},
url = {{http://dx.doi.org/10.3390/microorganisms9030531}},
doi = {{10.3390/microorganisms9030531}},
volume = {{9}},
year = {{2021}},
}