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Effects of 20-minute warm ischemia on coronary endothelial function of pig donor hearts after cardiac death

Sun, Xiao Ke ; Zhang, Wei Hua ; Qin, Guang Qi LU ; Liao, Qiu Ming ; Stig, Steen and Qiao, Chen Hui (2016) In Chinese Journal of Tissue Engineering Research 20(29). p.4362-4367
Abstract

BACKGROUND: Myocardial and coronary endothelial injury occurs in donor hearts due to warm ischemia during cardiac transplantation. Coronary endothelial structure and function play a critical role in long-term outcomes for patients after cardiac transplantation. OBJECTIVE: To study the effect of hypoxia-induced warm ischemia (20 minutes) on coronary endothelial function of porcine donor hearts after cardiac death (DCD). METHODS: Sixteen healthy Swedish domestic pigs were randomized into control (n=6), DCD (n=5), and DCD plus cold storage (n=5) groups, respectively. A DCD model in pigs was established using the method of hypoxia-induced 20-minute warm ischemia in the DCD and DCD plus cold storage groups. Isolation of the heart left... (More)

BACKGROUND: Myocardial and coronary endothelial injury occurs in donor hearts due to warm ischemia during cardiac transplantation. Coronary endothelial structure and function play a critical role in long-term outcomes for patients after cardiac transplantation. OBJECTIVE: To study the effect of hypoxia-induced warm ischemia (20 minutes) on coronary endothelial function of porcine donor hearts after cardiac death (DCD). METHODS: Sixteen healthy Swedish domestic pigs were randomized into control (n=6), DCD (n=5), and DCD plus cold storage (n=5) groups, respectively. A DCD model in pigs was established using the method of hypoxia-induced 20-minute warm ischemia in the DCD and DCD plus cold storage groups. Isolation of the heart left anterior descending coronary artery or combined with heart preservation pretreatment for 4 hours was performed in the DCD and DCD plus cold storage groups. The maximum coronary endothelium-dependent relaxation was determined in the three groups. RESULTS AND CONCLUSION: There were no significant differences in the maximum coronary endothelium-dependent relaxation and the minus logarithmic of substance concentration induced 50%maximal relaxation among three groups (P > 0.05). These results indicate that 20-minute warm ischemia cannot lead to obvious coronary endothelial dysfunction. In addition, DCD combined with 4-hour cold storage does not affect coronary endothelial function.

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author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Endothelial Cells, Heart, Swine, Transplantation
in
Chinese Journal of Tissue Engineering Research
volume
20
issue
29
article number
2095-4344(2016)29-04362-06
pages
6 pages
publisher
Journal of Clinical Rehabilitative Tissue Engineering Research
external identifiers
  • scopus:85086087203
ISSN
2095-4344
DOI
10.3969/j.issn.2095-4344.2016.29.015
language
English
LU publication?
no
id
97d9308a-8593-49be-afe5-fc42c7698d47
date added to LUP
2020-06-26 13:53:25
date last changed
2022-06-14 05:02:12
@article{97d9308a-8593-49be-afe5-fc42c7698d47,
  abstract     = {{<p>BACKGROUND: Myocardial and coronary endothelial injury occurs in donor hearts due to warm ischemia during cardiac transplantation. Coronary endothelial structure and function play a critical role in long-term outcomes for patients after cardiac transplantation. OBJECTIVE: To study the effect of hypoxia-induced warm ischemia (20 minutes) on coronary endothelial function of porcine donor hearts after cardiac death (DCD). METHODS: Sixteen healthy Swedish domestic pigs were randomized into control (n=6), DCD (n=5), and DCD plus cold storage (n=5) groups, respectively. A DCD model in pigs was established using the method of hypoxia-induced 20-minute warm ischemia in the DCD and DCD plus cold storage groups. Isolation of the heart left anterior descending coronary artery or combined with heart preservation pretreatment for 4 hours was performed in the DCD and DCD plus cold storage groups. The maximum coronary endothelium-dependent relaxation was determined in the three groups. RESULTS AND CONCLUSION: There were no significant differences in the maximum coronary endothelium-dependent relaxation and the minus logarithmic of substance concentration induced 50%maximal relaxation among three groups (P &gt; 0.05). These results indicate that 20-minute warm ischemia cannot lead to obvious coronary endothelial dysfunction. In addition, DCD combined with 4-hour cold storage does not affect coronary endothelial function.</p>}},
  author       = {{Sun, Xiao Ke and Zhang, Wei Hua and Qin, Guang Qi and Liao, Qiu Ming and Stig, Steen and Qiao, Chen Hui}},
  issn         = {{2095-4344}},
  keywords     = {{Endothelial Cells; Heart; Swine; Transplantation}},
  language     = {{eng}},
  number       = {{29}},
  pages        = {{4362--4367}},
  publisher    = {{Journal of Clinical Rehabilitative Tissue Engineering Research}},
  series       = {{Chinese Journal of Tissue Engineering Research}},
  title        = {{Effects of 20-minute warm ischemia on coronary endothelial function of pig donor hearts after cardiac death}},
  url          = {{http://dx.doi.org/10.3969/j.issn.2095-4344.2016.29.015}},
  doi          = {{10.3969/j.issn.2095-4344.2016.29.015}},
  volume       = {{20}},
  year         = {{2016}},
}