Effects of 20-minute warm ischemia on coronary endothelial function of pig donor hearts after cardiac death
(2016) In Chinese Journal of Tissue Engineering Research 20(29). p.4362-4367- Abstract
BACKGROUND: Myocardial and coronary endothelial injury occurs in donor hearts due to warm ischemia during cardiac transplantation. Coronary endothelial structure and function play a critical role in long-term outcomes for patients after cardiac transplantation. OBJECTIVE: To study the effect of hypoxia-induced warm ischemia (20 minutes) on coronary endothelial function of porcine donor hearts after cardiac death (DCD). METHODS: Sixteen healthy Swedish domestic pigs were randomized into control (n=6), DCD (n=5), and DCD plus cold storage (n=5) groups, respectively. A DCD model in pigs was established using the method of hypoxia-induced 20-minute warm ischemia in the DCD and DCD plus cold storage groups. Isolation of the heart left... (More)
BACKGROUND: Myocardial and coronary endothelial injury occurs in donor hearts due to warm ischemia during cardiac transplantation. Coronary endothelial structure and function play a critical role in long-term outcomes for patients after cardiac transplantation. OBJECTIVE: To study the effect of hypoxia-induced warm ischemia (20 minutes) on coronary endothelial function of porcine donor hearts after cardiac death (DCD). METHODS: Sixteen healthy Swedish domestic pigs were randomized into control (n=6), DCD (n=5), and DCD plus cold storage (n=5) groups, respectively. A DCD model in pigs was established using the method of hypoxia-induced 20-minute warm ischemia in the DCD and DCD plus cold storage groups. Isolation of the heart left anterior descending coronary artery or combined with heart preservation pretreatment for 4 hours was performed in the DCD and DCD plus cold storage groups. The maximum coronary endothelium-dependent relaxation was determined in the three groups. RESULTS AND CONCLUSION: There were no significant differences in the maximum coronary endothelium-dependent relaxation and the minus logarithmic of substance concentration induced 50%maximal relaxation among three groups (P > 0.05). These results indicate that 20-minute warm ischemia cannot lead to obvious coronary endothelial dysfunction. In addition, DCD combined with 4-hour cold storage does not affect coronary endothelial function.
(Less)
- author
- Sun, Xiao Ke ; Zhang, Wei Hua ; Qin, Guang Qi LU ; Liao, Qiu Ming ; Stig, Steen and Qiao, Chen Hui
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Endothelial Cells, Heart, Swine, Transplantation
- in
- Chinese Journal of Tissue Engineering Research
- volume
- 20
- issue
- 29
- article number
- 2095-4344(2016)29-04362-06
- pages
- 6 pages
- publisher
- Journal of Clinical Rehabilitative Tissue Engineering Research
- external identifiers
-
- scopus:85086087203
- ISSN
- 2095-4344
- DOI
- 10.3969/j.issn.2095-4344.2016.29.015
- language
- English
- LU publication?
- no
- id
- 97d9308a-8593-49be-afe5-fc42c7698d47
- date added to LUP
- 2020-06-26 13:53:25
- date last changed
- 2022-06-14 05:02:12
@article{97d9308a-8593-49be-afe5-fc42c7698d47, abstract = {{<p>BACKGROUND: Myocardial and coronary endothelial injury occurs in donor hearts due to warm ischemia during cardiac transplantation. Coronary endothelial structure and function play a critical role in long-term outcomes for patients after cardiac transplantation. OBJECTIVE: To study the effect of hypoxia-induced warm ischemia (20 minutes) on coronary endothelial function of porcine donor hearts after cardiac death (DCD). METHODS: Sixteen healthy Swedish domestic pigs were randomized into control (n=6), DCD (n=5), and DCD plus cold storage (n=5) groups, respectively. A DCD model in pigs was established using the method of hypoxia-induced 20-minute warm ischemia in the DCD and DCD plus cold storage groups. Isolation of the heart left anterior descending coronary artery or combined with heart preservation pretreatment for 4 hours was performed in the DCD and DCD plus cold storage groups. The maximum coronary endothelium-dependent relaxation was determined in the three groups. RESULTS AND CONCLUSION: There were no significant differences in the maximum coronary endothelium-dependent relaxation and the minus logarithmic of substance concentration induced 50%maximal relaxation among three groups (P > 0.05). These results indicate that 20-minute warm ischemia cannot lead to obvious coronary endothelial dysfunction. In addition, DCD combined with 4-hour cold storage does not affect coronary endothelial function.</p>}}, author = {{Sun, Xiao Ke and Zhang, Wei Hua and Qin, Guang Qi and Liao, Qiu Ming and Stig, Steen and Qiao, Chen Hui}}, issn = {{2095-4344}}, keywords = {{Endothelial Cells; Heart; Swine; Transplantation}}, language = {{eng}}, number = {{29}}, pages = {{4362--4367}}, publisher = {{Journal of Clinical Rehabilitative Tissue Engineering Research}}, series = {{Chinese Journal of Tissue Engineering Research}}, title = {{Effects of 20-minute warm ischemia on coronary endothelial function of pig donor hearts after cardiac death}}, url = {{http://dx.doi.org/10.3969/j.issn.2095-4344.2016.29.015}}, doi = {{10.3969/j.issn.2095-4344.2016.29.015}}, volume = {{20}}, year = {{2016}}, }