An ELISA for hCAP-18, the cathelicidin present in human neutrophils and plasma
(1997) In Journal of Immunological Methods 206(1-2). p.53-59- Abstract
hCAP-18 is a newly described protein of human neutrophilic granulocytes which belongs to the cathelicidin family of antimicrobial proteins. Members of this protein family share a common N-terminal sequence followed by a highly diverse antimicrobial, cationic C-terminus. The present work describes the production of recombinant hCAP-18, the generation of antibodies to the protein and the development of an accurate, sensitive and specific ELISA for the detection of hCAP-18 in cells, plasma and urine with a detection limit of 0.084 ng/ml. The amount of hCAP-18 in neutrophils is 0.627 microgram protein per 10(6) cells. The plasma level is 1.18 micrograms/ml which is several fold higher than for other neutrophil specific granule proteins.... (More)
hCAP-18 is a newly described protein of human neutrophilic granulocytes which belongs to the cathelicidin family of antimicrobial proteins. Members of this protein family share a common N-terminal sequence followed by a highly diverse antimicrobial, cationic C-terminus. The present work describes the production of recombinant hCAP-18, the generation of antibodies to the protein and the development of an accurate, sensitive and specific ELISA for the detection of hCAP-18 in cells, plasma and urine with a detection limit of 0.084 ng/ml. The amount of hCAP-18 in neutrophils is 0.627 microgram protein per 10(6) cells. The plasma level is 1.18 micrograms/ml which is several fold higher than for other neutrophil specific granule proteins. hCAP-18 is present in plasma as high molecular weight complexes. In accordance with this, hCAP-18 is barely excreted in the urine. The bone marrow appears to be the major source of plasma hCAP-18. The high level of hCAP-18 in plasma may provide an important defense against microorganisms and endotoxins.
(Less)
- author
- Sørensen, O LU ; Cowland, J B ; Askaa, J and Borregaard, N
- publishing date
- 1997-08-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Anti-Infective Agents/blood, Antibodies/chemistry, Antimicrobial Cationic Peptides, Carrier Proteins/blood, Cathelicidins, Enzyme-Linked Immunosorbent Assay/methods, Humans, Neutrophils/chemistry, Plasma/chemistry, Rabbits, Reproducibility of Results, Sensitivity and Specificity
- in
- Journal of Immunological Methods
- volume
- 206
- issue
- 1-2
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:9328568
- scopus:0030805339
- ISSN
- 0022-1759
- DOI
- 10.1016/s0022-1759(97)00084-7
- language
- English
- LU publication?
- no
- id
- 97e44d17-9349-4534-90b5-56938a844d0f
- date added to LUP
- 2020-11-05 13:43:56
- date last changed
- 2024-04-05 15:09:40
@article{97e44d17-9349-4534-90b5-56938a844d0f,
abstract = {{<p>hCAP-18 is a newly described protein of human neutrophilic granulocytes which belongs to the cathelicidin family of antimicrobial proteins. Members of this protein family share a common N-terminal sequence followed by a highly diverse antimicrobial, cationic C-terminus. The present work describes the production of recombinant hCAP-18, the generation of antibodies to the protein and the development of an accurate, sensitive and specific ELISA for the detection of hCAP-18 in cells, plasma and urine with a detection limit of 0.084 ng/ml. The amount of hCAP-18 in neutrophils is 0.627 microgram protein per 10(6) cells. The plasma level is 1.18 micrograms/ml which is several fold higher than for other neutrophil specific granule proteins. hCAP-18 is present in plasma as high molecular weight complexes. In accordance with this, hCAP-18 is barely excreted in the urine. The bone marrow appears to be the major source of plasma hCAP-18. The high level of hCAP-18 in plasma may provide an important defense against microorganisms and endotoxins.</p>}},
author = {{Sørensen, O and Cowland, J B and Askaa, J and Borregaard, N}},
issn = {{0022-1759}},
keywords = {{Animals; Anti-Infective Agents/blood; Antibodies/chemistry; Antimicrobial Cationic Peptides; Carrier Proteins/blood; Cathelicidins; Enzyme-Linked Immunosorbent Assay/methods; Humans; Neutrophils/chemistry; Plasma/chemistry; Rabbits; Reproducibility of Results; Sensitivity and Specificity}},
language = {{eng}},
month = {{08}},
number = {{1-2}},
pages = {{53--59}},
publisher = {{Elsevier}},
series = {{Journal of Immunological Methods}},
title = {{An ELISA for hCAP-18, the cathelicidin present in human neutrophils and plasma}},
url = {{http://dx.doi.org/10.1016/s0022-1759(97)00084-7}},
doi = {{10.1016/s0022-1759(97)00084-7}},
volume = {{206}},
year = {{1997}},
}