Estrogenic activity of surface waters using zebrafish- and human-based in vitro assays : The Danube as a case-study
(2020) In Environmental Toxicology and Pharmacology 78.- Abstract
- Most in vitro reporter gene assays used to assess estrogenic contamination are based on human estrogen receptor α (hERα) activation. However, fish bioassays can have distinct response to estrogenic chemicals and mixtures, questioning the relevance of human-based bioassays for assessing risk to this species. In this study, zebrafish liver cells stably expressing zebrafish ERβ2 (ZELHβ2) and human breast cancer cells expressing hERα (MELN) were used to quantify the estrogenic activity of 25 surface water samples of the Danube River, for which chemicals have been previously quantified. Most samples had a low estrogenic activity below 0.1 ng/L 17β-estradiol-equivalents that was more often detected by MELN cells, while ZELHβ2 response tend to be... (More)
- Most in vitro reporter gene assays used to assess estrogenic contamination are based on human estrogen receptor α (hERα) activation. However, fish bioassays can have distinct response to estrogenic chemicals and mixtures, questioning the relevance of human-based bioassays for assessing risk to this species. In this study, zebrafish liver cells stably expressing zebrafish ERβ2 (ZELHβ2) and human breast cancer cells expressing hERα (MELN) were used to quantify the estrogenic activity of 25 surface water samples of the Danube River, for which chemicals have been previously quantified. Most samples had a low estrogenic activity below 0.1 ng/L 17β-estradiol-equivalents that was more often detected by MELN cells, while ZELHβ2 response tend to be lower than predicted based on the chemicals identified. Nevertheless, both bioassays quantified well a higher estrogenic activity at two sites, which was confirmed in vivo using a transgenic zebrafish assay. The results are discussed considering the effect-based trigger values proposed for water quality monitoring. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/982e6c10-19c5-41ee-a7e9-3c732edc5953
- author
- Serra, Hélène LU ; Brion, François ; Chardon, Clémence ; Budzinski, Hélène ; Schulze, Tobias ; Brack, Werner and Aït-aïssa, Selim
- organization
- publishing date
- 2020-05-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Environmental Toxicology and Pharmacology
- volume
- 78
- article number
- 103401
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:32417722
- scopus:85084458241
- ISSN
- 1382-6689
- DOI
- 10.1016/j.etap.2020.103401
- language
- English
- LU publication?
- yes
- id
- 982e6c10-19c5-41ee-a7e9-3c732edc5953
- date added to LUP
- 2020-05-19 12:19:56
- date last changed
- 2022-04-18 22:22:37
@article{982e6c10-19c5-41ee-a7e9-3c732edc5953, abstract = {{Most in vitro reporter gene assays used to assess estrogenic contamination are based on human estrogen receptor α (hERα) activation. However, fish bioassays can have distinct response to estrogenic chemicals and mixtures, questioning the relevance of human-based bioassays for assessing risk to this species. In this study, zebrafish liver cells stably expressing zebrafish ERβ2 (ZELHβ2) and human breast cancer cells expressing hERα (MELN) were used to quantify the estrogenic activity of 25 surface water samples of the Danube River, for which chemicals have been previously quantified. Most samples had a low estrogenic activity below 0.1 ng/L 17β-estradiol-equivalents that was more often detected by MELN cells, while ZELHβ2 response tend to be lower than predicted based on the chemicals identified. Nevertheless, both bioassays quantified well a higher estrogenic activity at two sites, which was confirmed in vivo using a transgenic zebrafish assay. The results are discussed considering the effect-based trigger values proposed for water quality monitoring.}}, author = {{Serra, Hélène and Brion, François and Chardon, Clémence and Budzinski, Hélène and Schulze, Tobias and Brack, Werner and Aït-aïssa, Selim}}, issn = {{1382-6689}}, language = {{eng}}, month = {{05}}, publisher = {{Elsevier}}, series = {{Environmental Toxicology and Pharmacology}}, title = {{Estrogenic activity of surface waters using zebrafish- and human-based in vitro assays : The Danube as a case-study}}, url = {{http://dx.doi.org/10.1016/j.etap.2020.103401}}, doi = {{10.1016/j.etap.2020.103401}}, volume = {{78}}, year = {{2020}}, }