Evaluating macrophage migration inhibitory factor 1 expression as a prognostic biomarker in colon cancer
(2020) In Tumor Biology 42(6).- Abstract
Objective: Several studies indicate that macrophage migration inhibitory factor 1 plays a role for tumor progression in colon cancer. We investigated whether determination of migration inhibitory factor 1 mRNA expression levels in lymph nodes of colon cancer patients could be used as a prognostic marker. Methods: Expression levels of migration inhibitory factor 1 and carcinoembryonic antigen mRNAs were assessed in primary tumors and regional lymph nodes of 123 colon cancer patients (stages I–IV), and in colon cancer- and immune cell lines using quantitative reverse transcriptase–polymerase chain reaction. Expression of migration inhibitory factor 1 protein was investigated by two-color immunohistochemistry and immunomorphometry.... (More)
Objective: Several studies indicate that macrophage migration inhibitory factor 1 plays a role for tumor progression in colon cancer. We investigated whether determination of migration inhibitory factor 1 mRNA expression levels in lymph nodes of colon cancer patients could be used as a prognostic marker. Methods: Expression levels of migration inhibitory factor 1 and carcinoembryonic antigen mRNAs were assessed in primary tumors and regional lymph nodes of 123 colon cancer patients (stages I–IV), and in colon cancer- and immune cell lines using quantitative reverse transcriptase–polymerase chain reaction. Expression of migration inhibitory factor 1 protein was investigated by two-color immunohistochemistry and immunomorphometry. Results: Migration inhibitory factor 1 mRNA was expressed at 60 times higher levels in primary colon cancer tumors compared to normal colonic tissue (medians 8.2 and 0.2 mRNA copies/18S rRNA unit; p <.0001). A highly significant difference in mRNA expression levels was found between hematoxylin-eosin positive lymph nodes and hematoxylin-eosin negative lymph nodes (p <.0001). Migration inhibitory factor 1 and carcinoembryonic antigen proteins were simultaneously expressed in many colon cancer-tumor cells. Kaplan–Meier survival model and hazard ratio analysis, using a cutoff level at 2.19 mRNA copies/18S rRNA unit, revealed that patients with lymph nodes expressing high levels of migration inhibitory factor 1 mRNA had a 3.5-fold (p =.04) higher risk for recurrence, associated with a small, but significant, difference in mean survival time (7 months, p =.03) at 12 years of follow-up. Conclusion: Although migration inhibitory factor 1 mRNA expression levels were related to severity of disease and lymph node analysis revealed that colon cancer patients with high levels had a shorter survival time after surgery than those with low levels, the difference was small and probably not useful in clinical practice.
(Less)
- author
- Olsson, Lina ; Lindmark, Gudrun LU ; Hammarström, Marie Louise ; Hammarström, Sten and Sitohy, Basel
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- carcinoembryonic antigen, colon cancer, cumulative survival curves; recurrence risk, disseminated tumor cells, immunofluorescence, immunohistochemistry, immunomorphometry, Macrophage migration inhibitory factor, quantitative reverse transcriptase–polymerase chain reaction
- in
- Tumor Biology
- volume
- 42
- issue
- 6
- publisher
- Springer
- external identifiers
-
- pmid:32515296
- scopus:85086257481
- ISSN
- 1010-4283
- DOI
- 10.1177/1010428320924524
- language
- English
- LU publication?
- yes
- id
- 987cd8b4-0736-43ae-8318-0eced1f1c664
- date added to LUP
- 2020-06-30 15:43:13
- date last changed
- 2024-03-20 12:08:20
@article{987cd8b4-0736-43ae-8318-0eced1f1c664,
abstract = {{<p>Objective: Several studies indicate that macrophage migration inhibitory factor 1 plays a role for tumor progression in colon cancer. We investigated whether determination of migration inhibitory factor 1 mRNA expression levels in lymph nodes of colon cancer patients could be used as a prognostic marker. Methods: Expression levels of migration inhibitory factor 1 and carcinoembryonic antigen mRNAs were assessed in primary tumors and regional lymph nodes of 123 colon cancer patients (stages I–IV), and in colon cancer- and immune cell lines using quantitative reverse transcriptase–polymerase chain reaction. Expression of migration inhibitory factor 1 protein was investigated by two-color immunohistochemistry and immunomorphometry. Results: Migration inhibitory factor 1 mRNA was expressed at 60 times higher levels in primary colon cancer tumors compared to normal colonic tissue (medians 8.2 and 0.2 mRNA copies/18S rRNA unit; p <.0001). A highly significant difference in mRNA expression levels was found between hematoxylin-eosin positive lymph nodes and hematoxylin-eosin negative lymph nodes (p <.0001). Migration inhibitory factor 1 and carcinoembryonic antigen proteins were simultaneously expressed in many colon cancer-tumor cells. Kaplan–Meier survival model and hazard ratio analysis, using a cutoff level at 2.19 mRNA copies/18S rRNA unit, revealed that patients with lymph nodes expressing high levels of migration inhibitory factor 1 mRNA had a 3.5-fold (p =.04) higher risk for recurrence, associated with a small, but significant, difference in mean survival time (7 months, p =.03) at 12 years of follow-up. Conclusion: Although migration inhibitory factor 1 mRNA expression levels were related to severity of disease and lymph node analysis revealed that colon cancer patients with high levels had a shorter survival time after surgery than those with low levels, the difference was small and probably not useful in clinical practice.</p>}},
author = {{Olsson, Lina and Lindmark, Gudrun and Hammarström, Marie Louise and Hammarström, Sten and Sitohy, Basel}},
issn = {{1010-4283}},
keywords = {{carcinoembryonic antigen; colon cancer; cumulative survival curves; recurrence risk; disseminated tumor cells; immunofluorescence; immunohistochemistry; immunomorphometry; Macrophage migration inhibitory factor; quantitative reverse transcriptase–polymerase chain reaction}},
language = {{eng}},
number = {{6}},
publisher = {{Springer}},
series = {{Tumor Biology}},
title = {{Evaluating macrophage migration inhibitory factor 1 expression as a prognostic biomarker in colon cancer}},
url = {{http://dx.doi.org/10.1177/1010428320924524}},
doi = {{10.1177/1010428320924524}},
volume = {{42}},
year = {{2020}},
}