Digital Examination of LYmph node CYtopathology Using the Sydney system (DELYCYUS). An international, multi-institutional study
(2023) In Cancer Cytopathology 131(11). p.679-692- Abstract
Background: After a series of standardized reporting systems in cytopathology, the Sydney system was recently introduced to address the need for reproducibility and standardization in lymph node cytopathology. Since then, the risk of malignancy for the categories of the Sydney system has been explored by several studies, but no studies have yet examined the interobserver reproducibility of the Sydney system. Methods: The authors assessed interobserver reproducibility of the Sydney system on 85 lymph node fine-needle aspiration cytology cases reviewed by 15 cytopathologists from 12 institutions in eight different countries, resulting in 1275 diagnoses. In total, 186 slides stained with Diff-Quik, Papanicolaou, and immunocytochemistry... (More)
Background: After a series of standardized reporting systems in cytopathology, the Sydney system was recently introduced to address the need for reproducibility and standardization in lymph node cytopathology. Since then, the risk of malignancy for the categories of the Sydney system has been explored by several studies, but no studies have yet examined the interobserver reproducibility of the Sydney system. Methods: The authors assessed interobserver reproducibility of the Sydney system on 85 lymph node fine-needle aspiration cytology cases reviewed by 15 cytopathologists from 12 institutions in eight different countries, resulting in 1275 diagnoses. In total, 186 slides stained with Diff-Quik, Papanicolaou, and immunocytochemistry were scanned. A subset of the cases included clinical data and results from ultrasound examinations, flow cytometry immunophenotyping, and fluorescence in situ hybridization analysis. The study participants assessed the cases digitally using whole-slide images. Results: Overall, the authors observed an almost perfect agreement of cytopathologists with the ground truth (median weighted Cohen κ = 0.887; interquartile range, κ = 0.210) and moderate overall interobserver concordance (Fleiss κ = 0.476). There was substantial agreement for the inadequate and malignant categories (κ = 0.794 and κ = 0.729, respectively), moderate agreement for the benign category (κ = 0.490), and very slight agreement for the suspicious (κ = 0.104) and atypical (κ = 0.075) categories. Conclusions: The Sydney system for reporting lymph node cytopathology shows adequate interobserver concordance. Digital microscopy is an adequate means to assess lymph node cytopathology specimens.
(Less)
- author
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- digital cytopathology, lymph node, reproducibility, virtual microscopy, whole-slide imaging
- in
- Cancer Cytopathology
- volume
- 131
- issue
- 11
- pages
- 679 - 692
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:37418195
- scopus:85164133837
- ISSN
- 1934-662X
- DOI
- 10.1002/cncy.22741
- language
- English
- LU publication?
- yes
- id
- 9893b635-fe11-4a0d-96bc-b9727323bb6f
- date added to LUP
- 2023-10-16 14:30:42
- date last changed
- 2024-04-19 03:25:13
@article{9893b635-fe11-4a0d-96bc-b9727323bb6f,
abstract = {{<p>Background: After a series of standardized reporting systems in cytopathology, the Sydney system was recently introduced to address the need for reproducibility and standardization in lymph node cytopathology. Since then, the risk of malignancy for the categories of the Sydney system has been explored by several studies, but no studies have yet examined the interobserver reproducibility of the Sydney system. Methods: The authors assessed interobserver reproducibility of the Sydney system on 85 lymph node fine-needle aspiration cytology cases reviewed by 15 cytopathologists from 12 institutions in eight different countries, resulting in 1275 diagnoses. In total, 186 slides stained with Diff-Quik, Papanicolaou, and immunocytochemistry were scanned. A subset of the cases included clinical data and results from ultrasound examinations, flow cytometry immunophenotyping, and fluorescence in situ hybridization analysis. The study participants assessed the cases digitally using whole-slide images. Results: Overall, the authors observed an almost perfect agreement of cytopathologists with the ground truth (median weighted Cohen κ = 0.887; interquartile range, κ = 0.210) and moderate overall interobserver concordance (Fleiss κ = 0.476). There was substantial agreement for the inadequate and malignant categories (κ = 0.794 and κ = 0.729, respectively), moderate agreement for the benign category (κ = 0.490), and very slight agreement for the suspicious (κ = 0.104) and atypical (κ = 0.075) categories. Conclusions: The Sydney system for reporting lymph node cytopathology shows adequate interobserver concordance. Digital microscopy is an adequate means to assess lymph node cytopathology specimens.</p>}},
author = {{Caputo, Alessandro and Fraggetta, Filippo and Cretella, Pasquale and Cozzolino, Immacolata and Eccher, Albino and Girolami, Ilaria and Marletta, Stefano and Troncone, Giancarlo and Vigliar, Elena and Acanfora, Gennaro and Zarra, Karen Villar and Torres Rivas, Héctor Enrique and Fadda, Guido and Field, Andrew and Katz, Ruth and Vielh, Philippe and Eloy, Catarina and Rajwanshi, Arvind and Gupta, Nalini and Al-Abbadi, Mousa and Bustami, Nadwa and Arar, Tala and Calaminici, Maria and Raine, Juliet I. and Barroca, Helena and Canão, Pedro Amoroso and Ehinger, Mats and Rajabian, Nilofar and Dey, Pranab and Medeiros, L. Jeffrey and El Hussein, Siba and Lin, Oscar and D’Antonio, Antonio and Bode-Lesniewska, Beata and Rossi, Esther Diana and Zeppa, Pio}},
issn = {{1934-662X}},
keywords = {{digital cytopathology; lymph node; reproducibility; virtual microscopy; whole-slide imaging}},
language = {{eng}},
number = {{11}},
pages = {{679--692}},
publisher = {{Wiley-Blackwell}},
series = {{Cancer Cytopathology}},
title = {{Digital Examination of LYmph node CYtopathology Using the Sydney system (DELYCYUS). An international, multi-institutional study}},
url = {{http://dx.doi.org/10.1002/cncy.22741}},
doi = {{10.1002/cncy.22741}},
volume = {{131}},
year = {{2023}},
}