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Protein kinase C is translocated to cell membranes during cerebral ischemia

Cardell, Monika LU ; Bingren, Hu ; Wieloch, Tadeusz LU ; Zivin, Justin and Saitoh, Tsunao (1990) In Neuroscience Letters 119(2). p.228-232
Abstract

The subcellular distribution of PKC(α) and PKC(γ) was studied in homogenates of cerebral cortex from rats subjected to 10 and 15 min of ischemia and 15 min of ischemia followed by 1 h, 6 h, 24 h, 48 h, and 7 days of reperfusion. During ischemia no significant changes in the levels of PKC (α) were seen. During the first hour of reperfusion, a transient 2.5-fold (P < 0.05) increase in PKC(α) levels was observed in the particulate fraction. In contrast, a three-fold increase of PKC(γ) in the particulate fraction concomitant with a 40% decrease in the cytosol was noted during ischemia. In the postischemic phase the levels in the cytosol decreased to 35% of control values at 2 days following ischemia, with a concomitant decrease in the... (More)

The subcellular distribution of PKC(α) and PKC(γ) was studied in homogenates of cerebral cortex from rats subjected to 10 and 15 min of ischemia and 15 min of ischemia followed by 1 h, 6 h, 24 h, 48 h, and 7 days of reperfusion. During ischemia no significant changes in the levels of PKC (α) were seen. During the first hour of reperfusion, a transient 2.5-fold (P < 0.05) increase in PKC(α) levels was observed in the particulate fraction. In contrast, a three-fold increase of PKC(γ) in the particulate fraction concomitant with a 40% decrease in the cytosol was noted during ischemia. In the postischemic phase the levels in the cytosol decreased to 35% of control values at 2 days following ischemia, with a concomitant decrease in the particulate fraction to control levels. The redistribution of PKC to the cell membranes during and following ischemia could be due to ischemia induced receptor activation, increased levels of diacylglycerols, arachidonate and intracellular calcium, and may be of importance for the development of ischemic neuronal damage.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Activation, Downregulation, Ischemia, Neurodegeneration, Neuronal plasticity, Neuronal regeneration, Protein kinase C
in
Neuroscience Letters
volume
119
issue
2
pages
5 pages
publisher
Elsevier
external identifiers
  • pmid:2280899
  • scopus:0024993805
ISSN
0304-3940
DOI
10.1016/0304-3940(90)90840-6
language
English
LU publication?
yes
id
98b7bdd8-aa14-4f52-bb71-a7531774eeb0
date added to LUP
2019-06-13 17:43:24
date last changed
2024-01-01 10:31:51
@article{98b7bdd8-aa14-4f52-bb71-a7531774eeb0,
  abstract     = {{<p>The subcellular distribution of PKC(α) and PKC(γ) was studied in homogenates of cerebral cortex from rats subjected to 10 and 15 min of ischemia and 15 min of ischemia followed by 1 h, 6 h, 24 h, 48 h, and 7 days of reperfusion. During ischemia no significant changes in the levels of PKC (α) were seen. During the first hour of reperfusion, a transient 2.5-fold (P &lt; 0.05) increase in PKC(α) levels was observed in the particulate fraction. In contrast, a three-fold increase of PKC(γ) in the particulate fraction concomitant with a 40% decrease in the cytosol was noted during ischemia. In the postischemic phase the levels in the cytosol decreased to 35% of control values at 2 days following ischemia, with a concomitant decrease in the particulate fraction to control levels. The redistribution of PKC to the cell membranes during and following ischemia could be due to ischemia induced receptor activation, increased levels of diacylglycerols, arachidonate and intracellular calcium, and may be of importance for the development of ischemic neuronal damage.</p>}},
  author       = {{Cardell, Monika and Bingren, Hu and Wieloch, Tadeusz and Zivin, Justin and Saitoh, Tsunao}},
  issn         = {{0304-3940}},
  keywords     = {{Activation; Downregulation; Ischemia; Neurodegeneration; Neuronal plasticity; Neuronal regeneration; Protein kinase C}},
  language     = {{eng}},
  month        = {{11}},
  number       = {{2}},
  pages        = {{228--232}},
  publisher    = {{Elsevier}},
  series       = {{Neuroscience Letters}},
  title        = {{Protein kinase C is translocated to cell membranes during cerebral ischemia}},
  url          = {{http://dx.doi.org/10.1016/0304-3940(90)90840-6}},
  doi          = {{10.1016/0304-3940(90)90840-6}},
  volume       = {{119}},
  year         = {{1990}},
}