Proof-of-Concept of a Novel Cell Separation Technology Using Magnetic Agarose-Based Beads
Brechmann, Nils A. ; Jansson, Märta ; Hägg, Alice LU
; Hicks, Ryan
; Hyllner, Johan
; Eriksson, Kristofer
and Chotteau, Véronique
(2022)
In Magnetochemistry
8(3).
- Abstract
The safety of the cells used for Advanced Therapy Medicinal Products is crucial for patients. Reliable methods for the cell purification are very important for the commercialization of those new therapies. With the large production scale envisioned for commercialization, the cell isolation methods need to be efficient, robust, operationally simple and generic while ensuring cell biological functionality and safety. In this study, we used high magnetized magnetic agarose-based beads conjugated with protein A to develop a new method for cell separation. A high separation efficiency of 91% yield and consistent isolation performances were demonstrated using population mixtures of human mesenchymal stem cells and HER2+ SKBR3 cells... (More)
The safety of the cells used for Advanced Therapy Medicinal Products is crucial for patients. Reliable methods for the cell purification are very important for the commercialization of those new therapies. With the large production scale envisioned for commercialization, the cell isolation methods need to be efficient, robust, operationally simple and generic while ensuring cell biological functionality and safety. In this study, we used high magnetized magnetic agarose-based beads conjugated with protein A to develop a new method for cell separation. A high separation efficiency of 91% yield and consistent isolation performances were demonstrated using population mixtures of human mesenchymal stem cells and HER2+ SKBR3 cells (80:20, 70:30 and 30:70). Additionally, high robustness against mechanical stress and minimal unspecific binding obtained with the protein A base conjugated magnetic beads were significant advantages in comparison with the same magnetic microparticles where the antibodies were covalently conjugated. This study provided insights on features of large high magnetized microparticles, which is promising for the large-scale application of cell purification.
(Less)
- author
- Brechmann, Nils A.
; Jansson, Märta
; Hägg, Alice
LU
; Hicks, Ryan
; Hyllner, Johan
; Eriksson, Kristofer
and Chotteau, Véronique
- publishing date
- 2022-03
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cell separation, Cell sorting, HER2, HMSCs, Magnetic bead separation, SKBR3 cells
- in
- Magnetochemistry
- volume
- 8
- issue
- 3
- article number
- 34
- publisher
- MDPI AG
- external identifiers
-
- scopus:85126707911
- ISSN
- 2312-7481
- DOI
- 10.3390/magnetochemistry8030034
- language
- English
- LU publication?
- no
- additional info
- Funding Information: Funding: This study was supported by AdBIOPRO, Competence Centre for Advanced BioProduction by Continuous Processing (Grant/Award number: 2016-05181) and CAMP, Competence Centre for Advanced Medical Products (Grant/Award number: 2017-02130), both funded by the Swedish Agency for Innovation Systems VINNOVA. The APC was funded by KTH library. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
- id
- 99059043-ff40-47ef-af5a-4f0e556b91c4
- date added to LUP
- 2022-10-13 10:12:19
- date last changed
- 2024-05-16 18:27:11
@article{99059043-ff40-47ef-af5a-4f0e556b91c4,
abstract = {{<p>The safety of the cells used for Advanced Therapy Medicinal Products is crucial for patients. Reliable methods for the cell purification are very important for the commercialization of those new therapies. With the large production scale envisioned for commercialization, the cell isolation methods need to be efficient, robust, operationally simple and generic while ensuring cell biological functionality and safety. In this study, we used high magnetized magnetic agarose-based beads conjugated with protein A to develop a new method for cell separation. A high separation efficiency of 91% yield and consistent isolation performances were demonstrated using population mixtures of human mesenchymal stem cells and HER2<sup>+</sup> SKBR3 cells (80:20, 70:30 and 30:70). Additionally, high robustness against mechanical stress and minimal unspecific binding obtained with the protein A base conjugated magnetic beads were significant advantages in comparison with the same magnetic microparticles where the antibodies were covalently conjugated. This study provided insights on features of large high magnetized microparticles, which is promising for the large-scale application of cell purification.</p>}},
author = {{Brechmann, Nils A. and Jansson, Märta and Hägg, Alice and Hicks, Ryan and Hyllner, Johan and Eriksson, Kristofer and Chotteau, Véronique}},
issn = {{2312-7481}},
keywords = {{Cell separation; Cell sorting; HER2; HMSCs; Magnetic bead separation; SKBR3 cells}},
language = {{eng}},
number = {{3}},
publisher = {{MDPI AG}},
series = {{Magnetochemistry}},
title = {{Proof-of-Concept of a Novel Cell Separation Technology Using Magnetic Agarose-Based Beads}},
url = {{http://dx.doi.org/10.3390/magnetochemistry8030034}},
doi = {{10.3390/magnetochemistry8030034}},
volume = {{8}},
year = {{2022}},
}