Advanced

Early regeneration in vitro of adult mouse sciatic axons is dependent on local protein synthesis but may not involve neurotrophins

Edbladh, Magnus LU ; Tonge, David; Golding, Jon; Ekström, Per A R LU and Edström, Anders LU (1994) In Neuroscience Letters 168(1-2). p.37-40
Abstract

The sensory axons of the adult mouse sciatic nerve were shown to regenerate after a local test crush lesion in vitro in a serum-free medium. The average outgrowth distance of the leading axons after culturing for 3 days was 2.8 ± 0.1 mm, which was shorter than in vivo (3.8 ± 0.2 mm). With the use of a compartmentalised culture system we could show that regeneration was partially dependent on local protein synthesis in the injury region. The initial stages of regeneration did not seem to involve neurotrophins since both K252a and K252b, selective and nontoxic inhibitors of the neurotrophin actions, failed to inhibit axonal growth. The present in vitro model system offers favourable conditions to investigate the early events of the... (More)

The sensory axons of the adult mouse sciatic nerve were shown to regenerate after a local test crush lesion in vitro in a serum-free medium. The average outgrowth distance of the leading axons after culturing for 3 days was 2.8 ± 0.1 mm, which was shorter than in vivo (3.8 ± 0.2 mm). With the use of a compartmentalised culture system we could show that regeneration was partially dependent on local protein synthesis in the injury region. The initial stages of regeneration did not seem to involve neurotrophins since both K252a and K252b, selective and nontoxic inhibitors of the neurotrophin actions, failed to inhibit axonal growth. The present in vitro model system offers favourable conditions to investigate the early events of the regeneration process in an adult mammalian peripheral nerve. © 1994.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adult, In vitro, Mouse, Nerve regeneration, Sciatic nerve
in
Neuroscience Letters
volume
168
issue
1-2
pages
4 pages
publisher
Elsevier
external identifiers
  • scopus:0028327405
ISSN
0304-3940
DOI
10.1016/0304-3940(94)90410-3
language
English
LU publication?
yes
id
994d499d-edd3-4e0e-83b8-25ae9e9060ab
date added to LUP
2016-12-07 14:58:44
date last changed
2017-01-27 15:25:08
@article{994d499d-edd3-4e0e-83b8-25ae9e9060ab,
  abstract     = {<p>The sensory axons of the adult mouse sciatic nerve were shown to regenerate after a local test crush lesion in vitro in a serum-free medium. The average outgrowth distance of the leading axons after culturing for 3 days was 2.8 ± 0.1 mm, which was shorter than in vivo (3.8 ± 0.2 mm). With the use of a compartmentalised culture system we could show that regeneration was partially dependent on local protein synthesis in the injury region. The initial stages of regeneration did not seem to involve neurotrophins since both K252a and K252b, selective and nontoxic inhibitors of the neurotrophin actions, failed to inhibit axonal growth. The present in vitro model system offers favourable conditions to investigate the early events of the regeneration process in an adult mammalian peripheral nerve. © 1994.</p>},
  author       = {Edbladh, Magnus and Tonge, David and Golding, Jon and Ekström, Per A R and Edström, Anders},
  issn         = {0304-3940},
  keyword      = {Adult,In vitro,Mouse,Nerve regeneration,Sciatic nerve},
  language     = {eng},
  month        = {02},
  number       = {1-2},
  pages        = {37--40},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {Early regeneration in vitro of adult mouse sciatic axons is dependent on local protein synthesis but may not involve neurotrophins},
  url          = {http://dx.doi.org/10.1016/0304-3940(94)90410-3},
  volume       = {168},
  year         = {1994},
}