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Spatio-temporal interdependence of bacteria and phytoplankton during a baltic sea spring bloom

Bunse, Carina; Bertos-Fortis, Mireia; Sassenhagen, Ingrid LU ; Sildever, Sirje; Sjöqvist, Conny; Godhe, Anna LU ; Gross, Susanna; Kremp, Anke; Lips, Inga and Lundholm, Nina, et al. (2016) In Frontiers in Microbiology 7.
Abstract

In temperate systems, phytoplankton spring blooms deplete inorganic nutrients and are major sources of organic matter for the microbial loop. In response to phytoplankton exudates and environmental factors, heterotrophic microbial communities are highly dynamic and change their abundance and composition both on spatial and temporal scales. Yet, most of our understanding about these processes comes from laboratory model organism studies, mesocosm experiments or single temporal transects. Spatial-temporal studies examining interactions of phytoplankton blooms and bacterioplankton community composition and function, though being highly informative, are scarce. In this study, pelagic microbial community dynamics (bacteria and phytoplankton)... (More)

In temperate systems, phytoplankton spring blooms deplete inorganic nutrients and are major sources of organic matter for the microbial loop. In response to phytoplankton exudates and environmental factors, heterotrophic microbial communities are highly dynamic and change their abundance and composition both on spatial and temporal scales. Yet, most of our understanding about these processes comes from laboratory model organism studies, mesocosm experiments or single temporal transects. Spatial-temporal studies examining interactions of phytoplankton blooms and bacterioplankton community composition and function, though being highly informative, are scarce. In this study, pelagic microbial community dynamics (bacteria and phytoplankton) and environmental variables were monitored during a spring bloom across the Baltic Proper (two cruises between North Germany to Gulf of Finland). To test to what extent bacterioplankton community composition relates to the spring bloom, we used next generation amplicon sequencing of the 16S rRNA gene, phytoplankton diversity analysis based on microscopy counts and population genotyping of the dominating diatom Skeletonema marinoi. Several phytoplankton bloom related and environmental variables were identified to influence bacterial community composition. Members of Bacteroidetes and Alphaproteobacteria dominated the bacterial community composition but the bacterial groups showed no apparent correlation with direct bloom related variables. The less abundant bacterial phyla Actinobacteria, Planctomycetes, and Verrucomicrobia, on the other hand, were strongly associated with phytoplankton biomass, diatom:dinoflagellate ratio, and colored dissolved organic matter (cDOM). Many bacterial operational taxonomic units (OTUs) showed high niche specificities. For example, particular Bacteroidetes OTUs were associated with two distinct genetic clusters of S. marinoi. Our study revealed the complexity of interactions of bacterial taxa with inter- and intraspecific genetic variation in phytoplankton. Overall, our findings imply that biotic and abiotic factors during spring bloom influence bacterial community dynamics in a hierarchical manner.

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publication status
published
subject
keywords
16S rRNA, Bacterioplankton, Baltic Sea, Marine bacteria, Phytoplankton, Skeletonema marinoi, Spatio-temporal, Spring bloom
in
Frontiers in Microbiology
volume
7
pages
10 pages
publisher
Frontiers
external identifiers
  • scopus:84966263551
ISSN
1664-302X
DOI
10.3389/fmicb.2016.00517
language
English
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yes
id
99ebaf76-0456-4f91-b320-c5a97d2c1051
date added to LUP
2019-07-02 17:31:40
date last changed
2019-10-15 07:10:05
@article{99ebaf76-0456-4f91-b320-c5a97d2c1051,
  abstract     = {<p>In temperate systems, phytoplankton spring blooms deplete inorganic nutrients and are major sources of organic matter for the microbial loop. In response to phytoplankton exudates and environmental factors, heterotrophic microbial communities are highly dynamic and change their abundance and composition both on spatial and temporal scales. Yet, most of our understanding about these processes comes from laboratory model organism studies, mesocosm experiments or single temporal transects. Spatial-temporal studies examining interactions of phytoplankton blooms and bacterioplankton community composition and function, though being highly informative, are scarce. In this study, pelagic microbial community dynamics (bacteria and phytoplankton) and environmental variables were monitored during a spring bloom across the Baltic Proper (two cruises between North Germany to Gulf of Finland). To test to what extent bacterioplankton community composition relates to the spring bloom, we used next generation amplicon sequencing of the 16S rRNA gene, phytoplankton diversity analysis based on microscopy counts and population genotyping of the dominating diatom Skeletonema marinoi. Several phytoplankton bloom related and environmental variables were identified to influence bacterial community composition. Members of Bacteroidetes and Alphaproteobacteria dominated the bacterial community composition but the bacterial groups showed no apparent correlation with direct bloom related variables. The less abundant bacterial phyla Actinobacteria, Planctomycetes, and Verrucomicrobia, on the other hand, were strongly associated with phytoplankton biomass, diatom:dinoflagellate ratio, and colored dissolved organic matter (cDOM). Many bacterial operational taxonomic units (OTUs) showed high niche specificities. For example, particular Bacteroidetes OTUs were associated with two distinct genetic clusters of S. marinoi. Our study revealed the complexity of interactions of bacterial taxa with inter- and intraspecific genetic variation in phytoplankton. Overall, our findings imply that biotic and abiotic factors during spring bloom influence bacterial community dynamics in a hierarchical manner.</p>},
  articleno    = {7:517},
  author       = {Bunse, Carina and Bertos-Fortis, Mireia and Sassenhagen, Ingrid and Sildever, Sirje and Sjöqvist, Conny and Godhe, Anna and Gross, Susanna and Kremp, Anke and Lips, Inga and Lundholm, Nina and Rengefors, Karin and Sefbom, Josefin and Pinhassi, Jarone and Legrand, Catherine},
  issn         = {1664-302X},
  keyword      = {16S rRNA,Bacterioplankton,Baltic Sea,Marine bacteria,Phytoplankton,Skeletonema marinoi,Spatio-temporal,Spring bloom},
  language     = {eng},
  month        = {04},
  pages        = {10},
  publisher    = {Frontiers},
  series       = {Frontiers in Microbiology},
  title        = {Spatio-temporal interdependence of bacteria and phytoplankton during a baltic sea spring bloom},
  url          = {http://dx.doi.org/10.3389/fmicb.2016.00517},
  volume       = {7},
  year         = {2016},
}