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The role of annexin A1 in Candida albicans and Candida auris infections in murine neutrophils

Sanches, José Marcos LU ; Rossato, Luana ; Lice, Izabella ; Alves de Piloto Fernandes, Anna Maria ; Bueno Duarte, Gustavo Henrique ; Rosini Silva, Alex Aparecido ; de Melo Porcari, Andreia ; de Oliveira Carvalho, Patrícia and Gil, Cristiane Damas (2021) In Microbial Pathogenesis 150.
Abstract

Annexin A1 (AnxA1) is an anti-inflammatory protein expressed in various cell types, especially macrophages and neutrophils. Because neutrophils play important roles in infections and inflammatory processes and the relationship between AnxA1 and Candida spp. infections is not well-understood, our study examined whether AnxA1 can serve as a target protein for the regulation of the immune response during fungal infections. C57BL/6 wild-type (WT) and AnxA1 knockout (AnxA1-/-) peritoneal neutrophils were coinfected with Candida albicans or Candida auris for 4 h. AnxA1-/- neutrophils exhibited a marked increase in cyclooxygenase 2 (COX-2), phosphorylated extracellular signal-related kinase (ERK), p-38, and c-Jun N-terminal kinase (JNK) levels... (More)

Annexin A1 (AnxA1) is an anti-inflammatory protein expressed in various cell types, especially macrophages and neutrophils. Because neutrophils play important roles in infections and inflammatory processes and the relationship between AnxA1 and Candida spp. infections is not well-understood, our study examined whether AnxA1 can serve as a target protein for the regulation of the immune response during fungal infections. C57BL/6 wild-type (WT) and AnxA1 knockout (AnxA1-/-) peritoneal neutrophils were coinfected with Candida albicans or Candida auris for 4 h. AnxA1-/- neutrophils exhibited a marked increase in cyclooxygenase 2 (COX-2), phosphorylated extracellular signal-related kinase (ERK), p-38, and c-Jun N-terminal kinase (JNK) levels after coinfection with both Candida spp. A lipidomics approach showed that AnxA1 deficiency produced marked differences in the supernatant lipid profiles of both control neutrophils and neutrophils coinfected with Candida spp. compared with WT cells, especially the levels of glycerophospholipids and glycerolipids. Our results showed that endogenous AnxA1 regulates the neutrophil response under fungal infection conditions, altering lipid membrane organization and metabolism.

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author
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publishing date
type
Contribution to journal
publication status
published
subject
in
Microbial Pathogenesis
volume
150
article number
104689
publisher
Academic Press
external identifiers
  • pmid:33307121
  • scopus:85097456268
ISSN
1096-1208
DOI
10.1016/j.micpath.2020.104689
language
English
LU publication?
no
id
99f7c895-a157-4780-87dd-fd565a8901f6
date added to LUP
2021-03-31 14:03:00
date last changed
2024-09-21 18:08:29
@article{99f7c895-a157-4780-87dd-fd565a8901f6,
  abstract     = {{<p>Annexin A1 (AnxA1) is an anti-inflammatory protein expressed in various cell types, especially macrophages and neutrophils. Because neutrophils play important roles in infections and inflammatory processes and the relationship between AnxA1 and Candida spp. infections is not well-understood, our study examined whether AnxA1 can serve as a target protein for the regulation of the immune response during fungal infections. C57BL/6 wild-type (WT) and AnxA1 knockout (AnxA1-/-) peritoneal neutrophils were coinfected with Candida albicans or Candida auris for 4 h. AnxA1-/- neutrophils exhibited a marked increase in cyclooxygenase 2 (COX-2), phosphorylated extracellular signal-related kinase (ERK), p-38, and c-Jun N-terminal kinase (JNK) levels after coinfection with both Candida spp. A lipidomics approach showed that AnxA1 deficiency produced marked differences in the supernatant lipid profiles of both control neutrophils and neutrophils coinfected with Candida spp. compared with WT cells, especially the levels of glycerophospholipids and glycerolipids. Our results showed that endogenous AnxA1 regulates the neutrophil response under fungal infection conditions, altering lipid membrane organization and metabolism.</p>}},
  author       = {{Sanches, José Marcos and Rossato, Luana and Lice, Izabella and Alves de Piloto Fernandes, Anna Maria and Bueno Duarte, Gustavo Henrique and Rosini Silva, Alex Aparecido and de Melo Porcari, Andreia and de Oliveira Carvalho, Patrícia and Gil, Cristiane Damas}},
  issn         = {{1096-1208}},
  language     = {{eng}},
  publisher    = {{Academic Press}},
  series       = {{Microbial Pathogenesis}},
  title        = {{The role of annexin A1 in Candida albicans and Candida auris infections in murine neutrophils}},
  url          = {{http://dx.doi.org/10.1016/j.micpath.2020.104689}},
  doi          = {{10.1016/j.micpath.2020.104689}},
  volume       = {{150}},
  year         = {{2021}},
}