Characterization of the Weimberg Pathway in Caulobacter crescentus
(2018) In Fermentation 4(2).- Abstract
- Caulobacter crescentus is a gram-negative bacterium that can utilize xylose as a substrate using the Weimberg pathway, which converts xylose to α-ketoglutarate in five steps without carbon loss. This is an interesting pathway for heterologous expression in other organisms in order to enable xylose utilization in biorefinery processes. C. crescentus was grown on xylose, arabinose and glucose, and maximum specific growth rates determined for the three substrates were 0.11 h−1, 0.05 h−1, and 0.15 h−1 respectively. Growth was found to be significantly inhibited at sugar concentration of 20 g L−1, shown primarily by an increased lag phase. Enzyme activity assays showed that the Weimberg pathway was active in cells grown, not only on xylose but... (More)
- Caulobacter crescentus is a gram-negative bacterium that can utilize xylose as a substrate using the Weimberg pathway, which converts xylose to α-ketoglutarate in five steps without carbon loss. This is an interesting pathway for heterologous expression in other organisms in order to enable xylose utilization in biorefinery processes. C. crescentus was grown on xylose, arabinose and glucose, and maximum specific growth rates determined for the three substrates were 0.11 h−1, 0.05 h−1, and 0.15 h−1 respectively. Growth was found to be significantly inhibited at sugar concentration of 20 g L−1, shown primarily by an increased lag phase. Enzyme activity assays showed that the Weimberg pathway was active in cells grown, not only on xylose but also on arabinose. No activity was found for growth on glucose. Furthermore, substantial amounts of α-ketoglutarate—up to a yield of 0.4 g g−1—was excreted during growth on xylose, but no other extracellular intermediates in the Weimberg pathway were detected during growth on xylose. Apparently, C. crescentus is not well adapted for efficient growth on high xylose levels, and responds by an extended lag phase and secretion of α-ketoglutarate. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/9a579f65-0d82-4bb5-9e92-68412970d109
- author
- Almqvist, Henrik LU ; Jonsdottir Glaser, Sara LU ; Tufvegren, Celina LU ; Wasserstrom, Lisa LU and Lidén, Gunnar LU
- organization
- publishing date
- 2018-06-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Caulobacter crescentus, Weimberg pathway, xylose, arabinose, physiological characterization, enzyme activity
- in
- Fermentation
- volume
- 4
- issue
- 2
- article number
- 44
- pages
- 11 pages
- publisher
- MDPI AG
- external identifiers
-
- scopus:85062227335
- ISSN
- 2311-5637
- DOI
- 10.3390/fermentation4020044
- language
- English
- LU publication?
- yes
- id
- 9a579f65-0d82-4bb5-9e92-68412970d109
- date added to LUP
- 2018-06-18 09:46:36
- date last changed
- 2023-12-16 18:06:32
@article{9a579f65-0d82-4bb5-9e92-68412970d109,
abstract = {{Caulobacter crescentus is a gram-negative bacterium that can utilize xylose as a substrate using the Weimberg pathway, which converts xylose to α-ketoglutarate in five steps without carbon loss. This is an interesting pathway for heterologous expression in other organisms in order to enable xylose utilization in biorefinery processes. C. crescentus was grown on xylose, arabinose and glucose, and maximum specific growth rates determined for the three substrates were 0.11 h−1, 0.05 h−1, and 0.15 h−1 respectively. Growth was found to be significantly inhibited at sugar concentration of 20 g L−1, shown primarily by an increased lag phase. Enzyme activity assays showed that the Weimberg pathway was active in cells grown, not only on xylose but also on arabinose. No activity was found for growth on glucose. Furthermore, substantial amounts of α-ketoglutarate—up to a yield of 0.4 g g−1—was excreted during growth on xylose, but no other extracellular intermediates in the Weimberg pathway were detected during growth on xylose. Apparently, C. crescentus is not well adapted for efficient growth on high xylose levels, and responds by an extended lag phase and secretion of α-ketoglutarate.}},
author = {{Almqvist, Henrik and Jonsdottir Glaser, Sara and Tufvegren, Celina and Wasserstrom, Lisa and Lidén, Gunnar}},
issn = {{2311-5637}},
keywords = {{Caulobacter crescentus; Weimberg pathway; xylose; arabinose; physiological characterization; enzyme activity}},
language = {{eng}},
month = {{06}},
number = {{2}},
publisher = {{MDPI AG}},
series = {{Fermentation}},
title = {{Characterization of the Weimberg Pathway in Caulobacter crescentus}},
url = {{http://dx.doi.org/10.3390/fermentation4020044}},
doi = {{10.3390/fermentation4020044}},
volume = {{4}},
year = {{2018}},
}