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Hypoxia-inducible factor and vascular endothelial growth factor in the neuroretina and retinal blood vessels after retinal ischemia

Håkansson, Gisela; Gesslein, Bodil LU ; Gustafsson, Lotta LU ; Englund-Johansson, Ulrica LU and Malmsjö, Malin LU (2010) In Journal of Ocular Biology, Diseases, and Informatics 3(1). p.20-29
Abstract

Retinal ischemia arises from circulatory failure. As the retinal blood vessels are key organs in circulatory failure, our aim was to study the retinal vasculature separately from the neuroretina to elucidate the role of hypoxia-inducible factor (HIF) 1α and 1β and vascular endothelial growth factor (VEGF) in retinal ischemia. Retinal ischemia was induced in porcine eyes by applying an intraocular pressure, followed by 12 h of reperfusion. HIF-1α mRNA expression was not affected by ischemia, while immunofluorescence staining was higher after ischemia in the neuroretina. HIF-1β immu-noreactivity and mRNA expression were unaffected. VEGF protein levels in the vitreous humor and VEGF staining in the neuroretina were more pronounced in eyes... (More)

Retinal ischemia arises from circulatory failure. As the retinal blood vessels are key organs in circulatory failure, our aim was to study the retinal vasculature separately from the neuroretina to elucidate the role of hypoxia-inducible factor (HIF) 1α and 1β and vascular endothelial growth factor (VEGF) in retinal ischemia. Retinal ischemia was induced in porcine eyes by applying an intraocular pressure, followed by 12 h of reperfusion. HIF-1α mRNA expression was not affected by ischemia, while immunofluorescence staining was higher after ischemia in the neuroretina. HIF-1β immu-noreactivity and mRNA expression were unaffected. VEGF protein levels in the vitreous humor and VEGF staining in the neuroretina were more pronounced in eyes subjected to ischemia than in the sham eyes. VEGF may be activated downstream of HIF-1 and is known to stimulate retinal neovascularization, which causes sight-threatening complications. These results emphasize the need for pharmacological treatment to block the HIF and VEGF signaling pathways in retinal ischemia.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Blood vessels, Hypoxia-inducible factor, Ischemia, Porcine, Retina, Vascular endothelial growth factor
in
Journal of Ocular Biology, Diseases, and Informatics
volume
3
issue
1
pages
10 pages
publisher
Humana Press
external identifiers
  • scopus:78650213172
DOI
10.1007/s12177-010-9050-6
language
English
LU publication?
yes
id
9a789eff-9277-4a90-9f56-e53e964b388a
date added to LUP
2016-11-14 11:51:58
date last changed
2018-05-29 09:49:17
@article{9a789eff-9277-4a90-9f56-e53e964b388a,
  abstract     = {<p>Retinal ischemia arises from circulatory failure. As the retinal blood vessels are key organs in circulatory failure, our aim was to study the retinal vasculature separately from the neuroretina to elucidate the role of hypoxia-inducible factor (HIF) 1α and 1β and vascular endothelial growth factor (VEGF) in retinal ischemia. Retinal ischemia was induced in porcine eyes by applying an intraocular pressure, followed by 12 h of reperfusion. HIF-1α mRNA expression was not affected by ischemia, while immunofluorescence staining was higher after ischemia in the neuroretina. HIF-1β immu-noreactivity and mRNA expression were unaffected. VEGF protein levels in the vitreous humor and VEGF staining in the neuroretina were more pronounced in eyes subjected to ischemia than in the sham eyes. VEGF may be activated downstream of HIF-1 and is known to stimulate retinal neovascularization, which causes sight-threatening complications. These results emphasize the need for pharmacological treatment to block the HIF and VEGF signaling pathways in retinal ischemia.</p>},
  author       = {Håkansson, Gisela and Gesslein, Bodil and Gustafsson, Lotta and Englund-Johansson, Ulrica and Malmsjö, Malin},
  keyword      = {Blood vessels,Hypoxia-inducible factor,Ischemia,Porcine,Retina,Vascular endothelial growth factor},
  language     = {eng},
  number       = {1},
  pages        = {20--29},
  publisher    = {Humana Press},
  series       = {Journal of Ocular Biology, Diseases, and Informatics},
  title        = {Hypoxia-inducible factor and vascular endothelial growth factor in the neuroretina and retinal blood vessels after retinal ischemia},
  url          = {http://dx.doi.org/10.1007/s12177-010-9050-6},
  volume       = {3},
  year         = {2010},
}