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Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

Giobbe, Giovanni Giuseppe ; Crowley, Claire ; Luni, Camilla ; Campinoti, Sara ; Khedr, Moustafa ; Kretzschmar, Kai ; De Santis, Martina M. LU ; Zambaiti, Elisa ; Michielin, Federica and Meran, Laween , et al. (2019) In Nature Communications 10. p.1-14
Abstract
Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be... (More)
Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically. (Less)
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publishing date
type
Contribution to journal
publication status
published
in
Nature Communications
volume
10
article number
5658
pages
1 - 14
publisher
Nature Publishing Group
external identifiers
  • pmid:31827102
  • scopus:85076343514
ISSN
2041-1723
DOI
10.1038/s41467-019-13605-4
language
English
LU publication?
no
id
9a79e83f-7041-418e-99e9-dc2d51321140
date added to LUP
2020-01-08 11:15:06
date last changed
2020-06-03 05:15:41
@article{9a79e83f-7041-418e-99e9-dc2d51321140,
  abstract     = {Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.},
  author       = {Giobbe, Giovanni Giuseppe  and Crowley, Claire and Luni, Camilla and Campinoti, Sara and Khedr, Moustafa and Kretzschmar, Kai and De Santis, Martina M. and Zambaiti, Elisa and Michielin, Federica and Meran, Laween and Hu, Qianjiang and van Son, Gijs and Urbani, Luca and Manfredi, Anna and Giomo, Monica and  Eaton, Simon and Cacchiarelli, Davide and Li, Vivian S. W. and Clevers, Hans and Bonfanti, Paola and Elvassore, Nicola and De Coppi, Paolo},
  issn         = {2041-1723},
  language     = {eng},
  month        = {12},
  pages        = {1--14},
  publisher    = {Nature Publishing Group},
  series       = {Nature Communications},
  title        = {Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture},
  url          = {http://dx.doi.org/10.1038/s41467-019-13605-4},
  doi          = {10.1038/s41467-019-13605-4},
  volume       = {10},
  year         = {2019},
}