Advanced

Purification and partial characterization of rat liver soluble catechol- O-methyltransferase

Tilgmann, Carola LU and Kalkkinen, Nisse (1990) In FEBS Letters 264(1). p.95-99
Abstract

The rat liver soluble catechol-O-methyltransferase (EC 2.1.1.6.) has been purified utilizing a combination of conventional chromatography and HPLC. The purified enzyme has a molecular mass of 25 kDa, a pI of 5.1, and exists in two forms which differ in the nature of their intramolecular disulfide bonds. This difference causes these two protein forms to behave differently in reversed phase chromatography.

Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Catechol-O-methyltransferase, soluble, Methylating enzyme
in
FEBS Letters
volume
264
issue
1
pages
5 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0025355104
ISSN
0014-5793
DOI
10.1016/0014-5793(90)80774-D
language
English
LU publication?
no
id
9a9cc68d-8609-4e43-9884-a599a5e42c52
date added to LUP
2016-04-11 13:26:35
date last changed
2018-11-21 21:22:45
@article{9a9cc68d-8609-4e43-9884-a599a5e42c52,
  abstract     = {<p>The rat liver soluble catechol-O-methyltransferase (EC 2.1.1.6.) has been purified utilizing a combination of conventional chromatography and HPLC. The purified enzyme has a molecular mass of 25 kDa, a pI of 5.1, and exists in two forms which differ in the nature of their intramolecular disulfide bonds. This difference causes these two protein forms to behave differently in reversed phase chromatography.</p>},
  author       = {Tilgmann, Carola and Kalkkinen, Nisse},
  issn         = {0014-5793},
  keyword      = {Catechol-O-methyltransferase, soluble,Methylating enzyme},
  language     = {eng},
  month        = {05},
  number       = {1},
  pages        = {95--99},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Purification and partial characterization of rat liver soluble catechol- O-methyltransferase},
  url          = {http://dx.doi.org/10.1016/0014-5793(90)80774-D},
  volume       = {264},
  year         = {1990},
}