Evidence of excited state localization and static disorder in LH2 investigated by 2D-polarization single-molecule imaging at room temperature.
Tubasum, Sumera LU ; Camacho Dejay, Rafael LU ; Meyer, Matthias LU ; Yadav, Dheerendra LU ; Cogdell, Richard J ; Pullerits, Tönu LU and Scheblykin, Ivan LU
(2013)
In Physical Chemistry Chemical Physics
15(45).
p.19862-19869
- Abstract
- Two-dimensional polarization fluorescence imaging of single light harvesting complexes 2 (LH2) of Rps. acidophila was carried out to investigate the polarization properties of excitation and fluorescence emission simultaneously, at room temperature. In two separate experiments we excited LH2 with a spectrally narrow laser line matched to the absorption bands of the two chromophore rings, B800 and B850, thereby indirectly and directly triggering fluorescence of the B850 exciton state. A correlation analysis of the polarization modulation depths in excitation and emission for a large number of single complexes was performed. Our results show, in comparison to B800, that the B850 ring is a more isotropic absorber due to the excitonic nature... (More)
- Two-dimensional polarization fluorescence imaging of single light harvesting complexes 2 (LH2) of Rps. acidophila was carried out to investigate the polarization properties of excitation and fluorescence emission simultaneously, at room temperature. In two separate experiments we excited LH2 with a spectrally narrow laser line matched to the absorption bands of the two chromophore rings, B800 and B850, thereby indirectly and directly triggering fluorescence of the B850 exciton state. A correlation analysis of the polarization modulation depths in excitation and emission for a large number of single complexes was performed. Our results show, in comparison to B800, that the B850 ring is a more isotropic absorber due to the excitonic nature of its excited states. At the same time, we observed a strong tendency for LH2 to emit with dipolar character, from which preferential localization of the emissive exciton, stable for minutes, is inferred. We argue that the observed effects can consistently be explained by static energetic disorder and/or deformation of the complex, with possible involvement of exciton self-trapping. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4143126
- author
- Tubasum, Sumera
LU
; Camacho Dejay, Rafael
LU
; Meyer, Matthias
LU
; Yadav, Dheerendra
LU
; Cogdell, Richard J
; Pullerits, Tönu
LU
and Scheblykin, Ivan
LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Physical Chemistry Chemical Physics
- volume
- 15
- issue
- 45
- pages
- 19862 - 19869
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000326470200042
- pmid:24145962
- scopus:84887002837
- ISSN
- 1463-9084
- DOI
- 10.1039/c3cp52127c
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Chemical Physics (S) (011001060)
- id
- 9aae3a31-e1f4-4dda-9807-811e347719e9 (old id 4143126)
- date added to LUP
- 2016-04-01 10:47:09
- date last changed
- 2023-09-14 12:13:10
@article{9aae3a31-e1f4-4dda-9807-811e347719e9,
abstract = {{Two-dimensional polarization fluorescence imaging of single light harvesting complexes 2 (LH2) of Rps. acidophila was carried out to investigate the polarization properties of excitation and fluorescence emission simultaneously, at room temperature. In two separate experiments we excited LH2 with a spectrally narrow laser line matched to the absorption bands of the two chromophore rings, B800 and B850, thereby indirectly and directly triggering fluorescence of the B850 exciton state. A correlation analysis of the polarization modulation depths in excitation and emission for a large number of single complexes was performed. Our results show, in comparison to B800, that the B850 ring is a more isotropic absorber due to the excitonic nature of its excited states. At the same time, we observed a strong tendency for LH2 to emit with dipolar character, from which preferential localization of the emissive exciton, stable for minutes, is inferred. We argue that the observed effects can consistently be explained by static energetic disorder and/or deformation of the complex, with possible involvement of exciton self-trapping.}},
author = {{Tubasum, Sumera and Camacho Dejay, Rafael and Meyer, Matthias and Yadav, Dheerendra and Cogdell, Richard J and Pullerits, Tönu and Scheblykin, Ivan}},
issn = {{1463-9084}},
language = {{eng}},
number = {{45}},
pages = {{19862--19869}},
publisher = {{Royal Society of Chemistry}},
series = {{Physical Chemistry Chemical Physics}},
title = {{Evidence of excited state localization and static disorder in LH2 investigated by 2D-polarization single-molecule imaging at room temperature.}},
url = {{http://dx.doi.org/10.1039/c3cp52127c}},
doi = {{10.1039/c3cp52127c}},
volume = {{15}},
year = {{2013}},
}