Visualization of micropatterned complex biosensor sensing chemistries by means of scanning electrochemical microscopy
(2004) In Biosensors & Bioelectronics 19(10). p.1175-1184- Abstract
- Redox hydrogel-based micropatterned complex biosensor architectures, used as sensing chemistries in amperometric ethanol or glucose biosensors, were deposited on gold, graphite or glass. Well-localized immobilization of active hydrogels with variable compositions was achieved by dispensing 100 pl droplets of cocktails containing alcohol or glucose dehydrogenase, redox polymer (PVI(13)dmeOs) and crosslinker (PEGDGE) while moving the target surface relative to the position of the nozzle of a piezo-actuated microdispenser. The resulting structures were microscopic patterns of enzyme-containing lines of a redox hydrogel with a line width of about 100 mum. Scanning electrochemical microscopy (SECM) in the amperometric feedback mode was used to... (More)
- Redox hydrogel-based micropatterned complex biosensor architectures, used as sensing chemistries in amperometric ethanol or glucose biosensors, were deposited on gold, graphite or glass. Well-localized immobilization of active hydrogels with variable compositions was achieved by dispensing 100 pl droplets of cocktails containing alcohol or glucose dehydrogenase, redox polymer (PVI(13)dmeOs) and crosslinker (PEGDGE) while moving the target surface relative to the position of the nozzle of a piezo-actuated microdispenser. The resulting structures were microscopic patterns of enzyme-containing lines of a redox hydrogel with a line width of about 100 mum. Scanning electrochemical microscopy (SECM) in the amperometric feedback mode was used to visualize the immobilized enzyme microstructures and their localized biochemical activity was observed with high lateral resolution by detecting the enzymatically consumed substrate using K-4[Fe(CN)(6)] as a free-diffusing electron-transfer mediator. (C) 2003 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/138528
- author
- Niculescu, Mihaela ; Gaspar, Szilveszter LU ; Schulte, Albert ; Csöregi, Elisabeth LU and Schuhmann, Wolfgang
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Enzyme/redox-hydrogel microstructure, Microdispenser, Scanning electrochemical microscopy, SECM
- in
- Biosensors & Bioelectronics
- volume
- 19
- issue
- 10
- pages
- 1175 - 1184
- publisher
- Elsevier
- external identifiers
-
- pmid:15046748
- wos:000220702600006
- scopus:1642377016
- pmid:15046748
- ISSN
- 1873-4235
- DOI
- 10.1016/j.bios.2003.11.008
- language
- English
- LU publication?
- yes
- id
- 9b2ebaa5-4990-4c69-b470-620faefc58ba (old id 138528)
- date added to LUP
- 2016-04-01 16:31:18
- date last changed
- 2022-01-28 20:17:44
@article{9b2ebaa5-4990-4c69-b470-620faefc58ba, abstract = {{Redox hydrogel-based micropatterned complex biosensor architectures, used as sensing chemistries in amperometric ethanol or glucose biosensors, were deposited on gold, graphite or glass. Well-localized immobilization of active hydrogels with variable compositions was achieved by dispensing 100 pl droplets of cocktails containing alcohol or glucose dehydrogenase, redox polymer (PVI(13)dmeOs) and crosslinker (PEGDGE) while moving the target surface relative to the position of the nozzle of a piezo-actuated microdispenser. The resulting structures were microscopic patterns of enzyme-containing lines of a redox hydrogel with a line width of about 100 mum. Scanning electrochemical microscopy (SECM) in the amperometric feedback mode was used to visualize the immobilized enzyme microstructures and their localized biochemical activity was observed with high lateral resolution by detecting the enzymatically consumed substrate using K-4[Fe(CN)(6)] as a free-diffusing electron-transfer mediator. (C) 2003 Elsevier B.V. All rights reserved.}}, author = {{Niculescu, Mihaela and Gaspar, Szilveszter and Schulte, Albert and Csöregi, Elisabeth and Schuhmann, Wolfgang}}, issn = {{1873-4235}}, keywords = {{Enzyme/redox-hydrogel microstructure; Microdispenser; Scanning electrochemical microscopy; SECM}}, language = {{eng}}, number = {{10}}, pages = {{1175--1184}}, publisher = {{Elsevier}}, series = {{Biosensors & Bioelectronics}}, title = {{Visualization of micropatterned complex biosensor sensing chemistries by means of scanning electrochemical microscopy}}, url = {{http://dx.doi.org/10.1016/j.bios.2003.11.008}}, doi = {{10.1016/j.bios.2003.11.008}}, volume = {{19}}, year = {{2004}}, }