Modification upon aging of the density of presynaptic modulation systems in the hippocampus
Canas, Paula M ; Duarte, João M N LU
; Rodrigues, Ricardo J
; Köfalvi, Attila
and Cunha, Rodrigo A
(2009)
In Neurobiology of Aging
30(11).
p.84-1877
- Abstract
Different presynaptic neuromodulation systems have been explored as possible targets to manage neurodegenerative diseases. However, most studies used young adult animals whereas neurodegenerative diseases are prevalent in the elderly. Thus, we now explored by Western blot analysis how the density of different presynaptic markers and receptors changes with aging in rat hippocampal synaptosomes (purified nerve terminals). Compared to synaptosomal membranes from 2-month-old rats, the density of presynaptic proteins (synaptophysin or SNAP-25) decreased at 18-24 months. In parallel, markers of glutamatergic terminals (vGluT1 or vGluT2) and cholinergic terminal markers (vAChT) constantly decreased with aging from 12 to 18 months onwards,... (More)
Different presynaptic neuromodulation systems have been explored as possible targets to manage neurodegenerative diseases. However, most studies used young adult animals whereas neurodegenerative diseases are prevalent in the elderly. Thus, we now explored by Western blot analysis how the density of different presynaptic markers and receptors changes with aging in rat hippocampal synaptosomes (purified nerve terminals). Compared to synaptosomal membranes from 2-month-old rats, the density of presynaptic proteins (synaptophysin or SNAP-25) decreased at 18-24 months. In parallel, markers of glutamatergic terminals (vGluT1 or vGluT2) and cholinergic terminal markers (vAChT) constantly decreased with aging from 12 to 18 months onwards, whereas the densities of GABAergic (vGAT) only decreased after 24 months. Inhibitory A(1) and CB(1) receptor density tended to decrease with aging, whereas facilitatory mGluR5 and P2Y1 receptor density was roughly constant and facilitatory A(2A) receptor density increased at 18-24 months. Thus aging causes an imbalance of excitatory versus inhibitory nerve terminal markers and causes a predominant decrease of inhibitory rather than facilitatory presynaptic modulation systems.
(Less)
- author
- Canas, Paula M
; Duarte, João M N
LU
; Rodrigues, Ricardo J
; Köfalvi, Attila
and Cunha, Rodrigo A
- publishing date
- 2009-11
- type
- Contribution to journal
- publication status
- published
- keywords
- Age Factors, Aging/physiology, Animals, Gene Expression Regulation/physiology, Hippocampus/cytology, Male, Presynaptic Terminals/metabolism, Rats, Receptor, Adenosine A2A/metabolism, Receptor, Cannabinoid, CB1/metabolism, Receptors, Purinergic P2/metabolism, Receptors, Purinergic P2Y1, Synaptophysin/metabolism, Synaptosomal-Associated Protein 25/metabolism, Vesicular Acetylcholine Transport Proteins/metabolism, Vesicular Glutamate Transport Protein 1/metabolism, Vesicular Glutamate Transport Protein 2/metabolism, Vesicular Inhibitory Amino Acid Transport Proteins/metabolism
- in
- Neurobiology of Aging
- volume
- 30
- issue
- 11
- pages
- 84 - 1877
- publisher
- Elsevier
- external identifiers
-
- pmid:18304697
- scopus:70049083864
- ISSN
- 1558-1497
- DOI
- 10.1016/j.neurobiolaging.2008.01.003
- language
- English
- LU publication?
- no
- id
- 9b3ac988-8857-4094-9ed7-9a82b9bd362a
- date added to LUP
- 2019-02-22 09:17:35
- date last changed
- 2024-06-11 05:31:33
@article{9b3ac988-8857-4094-9ed7-9a82b9bd362a,
abstract = {{<p>Different presynaptic neuromodulation systems have been explored as possible targets to manage neurodegenerative diseases. However, most studies used young adult animals whereas neurodegenerative diseases are prevalent in the elderly. Thus, we now explored by Western blot analysis how the density of different presynaptic markers and receptors changes with aging in rat hippocampal synaptosomes (purified nerve terminals). Compared to synaptosomal membranes from 2-month-old rats, the density of presynaptic proteins (synaptophysin or SNAP-25) decreased at 18-24 months. In parallel, markers of glutamatergic terminals (vGluT1 or vGluT2) and cholinergic terminal markers (vAChT) constantly decreased with aging from 12 to 18 months onwards, whereas the densities of GABAergic (vGAT) only decreased after 24 months. Inhibitory A(1) and CB(1) receptor density tended to decrease with aging, whereas facilitatory mGluR5 and P2Y1 receptor density was roughly constant and facilitatory A(2A) receptor density increased at 18-24 months. Thus aging causes an imbalance of excitatory versus inhibitory nerve terminal markers and causes a predominant decrease of inhibitory rather than facilitatory presynaptic modulation systems.</p>}},
author = {{Canas, Paula M and Duarte, João M N and Rodrigues, Ricardo J and Köfalvi, Attila and Cunha, Rodrigo A}},
issn = {{1558-1497}},
keywords = {{Age Factors; Aging/physiology; Animals; Gene Expression Regulation/physiology; Hippocampus/cytology; Male; Presynaptic Terminals/metabolism; Rats; Receptor, Adenosine A2A/metabolism; Receptor, Cannabinoid, CB1/metabolism; Receptors, Purinergic P2/metabolism; Receptors, Purinergic P2Y1; Synaptophysin/metabolism; Synaptosomal-Associated Protein 25/metabolism; Vesicular Acetylcholine Transport Proteins/metabolism; Vesicular Glutamate Transport Protein 1/metabolism; Vesicular Glutamate Transport Protein 2/metabolism; Vesicular Inhibitory Amino Acid Transport Proteins/metabolism}},
language = {{eng}},
number = {{11}},
pages = {{84--1877}},
publisher = {{Elsevier}},
series = {{Neurobiology of Aging}},
title = {{Modification upon aging of the density of presynaptic modulation systems in the hippocampus}},
url = {{http://dx.doi.org/10.1016/j.neurobiolaging.2008.01.003}},
doi = {{10.1016/j.neurobiolaging.2008.01.003}},
volume = {{30}},
year = {{2009}},
}