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Bacterial growth and growth-limiting nutrients following chronic nitrogen additions to a hardwood forest soil

Kamble, Pramod LU ; Rousk, Johannes LU ; Frey, Serita D. and Bååth, Erland LU (2013) In Soil Biology & Biochemistry 59. p.32-37
Abstract
Increasing nitrogen(N) deposition due to anthropogenic activities has become a significant global change threat to N-poor terrestrial ecosystems. We compared bacterial growth and nutrients limiting bacterial growth in one of the longest running experiments on increasing N-deposition to a temperate forest, the Chronic Nitrogen Amendment Study at Harvard Forest, USA. Soil samples were collected in fall 2009 from the organic and mineral horizons of plots treated annually since 1988 with 0 (unfertilized), 50(low N) or 150 (high N) kg N ha(-1) as NH4NO3. In the organic horizon, bacterial growth (leucine incorporation) decreased by 5 times in the high N plots compared to the unfertilized treatment, while no decrease was observed in the mineral... (More)
Increasing nitrogen(N) deposition due to anthropogenic activities has become a significant global change threat to N-poor terrestrial ecosystems. We compared bacterial growth and nutrients limiting bacterial growth in one of the longest running experiments on increasing N-deposition to a temperate forest, the Chronic Nitrogen Amendment Study at Harvard Forest, USA. Soil samples were collected in fall 2009 from the organic and mineral horizons of plots treated annually since 1988 with 0 (unfertilized), 50(low N) or 150 (high N) kg N ha(-1) as NH4NO3. In the organic horizon, bacterial growth (leucine incorporation) decreased by 5 times in the high N plots compared to the unfertilized treatment, while no decrease was observed in the mineral horizon. Bacterial growth in all soils was primarily limited by lack of carbon (C), although adding only C (as glucose) resulted in only a minor increase in bacterial growth in the unfertilized soil compared to adding C in combination with N. The bacterial growth induced by adding only C increased with higher level of N fertilization, up to 7-8 times the level without any C addition in the high N treatment, suggesting increased availability of N for the bacteria with increasing N addition. (C) 2013 Elsevier Ltd. All rights reserved. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
keywords
N-deposition, Limiting factors, Bacterial growth, Leucine incorporation, N availability
in
Soil Biology & Biochemistry
volume
59
pages
32 - 37
publisher
Elsevier
external identifiers
  • wos:000318754000004
  • scopus:84873616488
ISSN
0038-0717
DOI
10.1016/j.soilbio.2012.12.017
language
English
LU publication?
yes
id
9b4b8eaa-3b48-4e1c-97c3-a21f43b17969 (old id 3935641)
date added to LUP
2016-04-01 13:14:38
date last changed
2020-01-12 11:34:42
@article{9b4b8eaa-3b48-4e1c-97c3-a21f43b17969,
  abstract     = {Increasing nitrogen(N) deposition due to anthropogenic activities has become a significant global change threat to N-poor terrestrial ecosystems. We compared bacterial growth and nutrients limiting bacterial growth in one of the longest running experiments on increasing N-deposition to a temperate forest, the Chronic Nitrogen Amendment Study at Harvard Forest, USA. Soil samples were collected in fall 2009 from the organic and mineral horizons of plots treated annually since 1988 with 0 (unfertilized), 50(low N) or 150 (high N) kg N ha(-1) as NH4NO3. In the organic horizon, bacterial growth (leucine incorporation) decreased by 5 times in the high N plots compared to the unfertilized treatment, while no decrease was observed in the mineral horizon. Bacterial growth in all soils was primarily limited by lack of carbon (C), although adding only C (as glucose) resulted in only a minor increase in bacterial growth in the unfertilized soil compared to adding C in combination with N. The bacterial growth induced by adding only C increased with higher level of N fertilization, up to 7-8 times the level without any C addition in the high N treatment, suggesting increased availability of N for the bacteria with increasing N addition. (C) 2013 Elsevier Ltd. All rights reserved.},
  author       = {Kamble, Pramod and Rousk, Johannes and Frey, Serita D. and Bååth, Erland},
  issn         = {0038-0717},
  language     = {eng},
  pages        = {32--37},
  publisher    = {Elsevier},
  series       = {Soil Biology & Biochemistry},
  title        = {Bacterial growth and growth-limiting nutrients following chronic nitrogen additions to a hardwood forest soil},
  url          = {http://dx.doi.org/10.1016/j.soilbio.2012.12.017},
  doi          = {10.1016/j.soilbio.2012.12.017},
  volume       = {59},
  year         = {2013},
}