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Monosynaptic retrograde tracing of neurons expressing the G-protein coupled receptor Gpr151 in the mouse brain

Broms, Jonas LU ; Grahm, Matilda LU ; Haugegaard, Lea; Blom, Thomas LU ; Meletis, Konstantinos and Tingström, Anders LU (2017) In Journal of Comparative Neurology 525(15). p.3227-3250
Abstract

GPR151 is a G-protein coupled receptor for which the endogenous ligand remains unknown. In the nervous system of vertebrates, its expression is enriched in specific diencephalic structures, where the highest levels are observed in the habenular area. The habenula has been implicated in a range of different functions including behavioral flexibility, decision making, inhibitory control, and pain processing, which makes it a promising target for treating psychiatric and neurological disease. This study aimed to further characterize neurons expressing the Gpr151 gene, by tracing the afferent connectivity of this diencephalic cell population. Using pseudotyped rabies virus in a transgenic Gpr151-Cre mouse line, monosynaptic afferents of... (More)

GPR151 is a G-protein coupled receptor for which the endogenous ligand remains unknown. In the nervous system of vertebrates, its expression is enriched in specific diencephalic structures, where the highest levels are observed in the habenular area. The habenula has been implicated in a range of different functions including behavioral flexibility, decision making, inhibitory control, and pain processing, which makes it a promising target for treating psychiatric and neurological disease. This study aimed to further characterize neurons expressing the Gpr151 gene, by tracing the afferent connectivity of this diencephalic cell population. Using pseudotyped rabies virus in a transgenic Gpr151-Cre mouse line, monosynaptic afferents of habenular and thalamic Gpr151-expressing neuronal populations could be visualized. The habenular and thalamic Gpr151 systems displayed both shared and distinct connectivity patterns. The habenular neurons primarily received input from basal forebrain structures, the bed nucleus of stria terminalis, the lateral preoptic area, the entopeduncular nucleus, and the lateral hypothalamic area. The Gpr151-expressing neurons in the paraventricular nucleus of the thalamus was primarily contacted by medial hypothalamic areas as well as the zona incerta and projected to specific forebrain areas such as the prelimbic cortex and the accumbens nucleus. Gpr151 mRNA was also detected at low levels in the lateral posterior thalamic nucleus which received input from areas associated with visual processing, including the superior colliculus, zona incerta, and the visual and retrosplenial cortices. Knowledge about the connectivity of Gpr151-expressing neurons will facilitate the interpretation of future functional studies of this receptor.

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published
subject
keywords
habenula, rabies, RRID: AB_10743815, RRID: AB_2571870, RRID: IMSR_JAX:000664, RRID: IMSR_JAX:024109, RRID: SCR_003070, thalamus
in
Journal of Comparative Neurology
volume
525
issue
15
pages
24 pages
publisher
John Wiley & Sons
external identifiers
  • scopus:85027535538
  • wos:000407820700004
ISSN
0021-9967
DOI
10.1002/cne.24273
language
English
LU publication?
yes
id
9b78f45a-f56b-4f0f-9153-bf8ca592a159
date added to LUP
2017-09-06 10:57:51
date last changed
2017-09-18 11:38:53
@article{9b78f45a-f56b-4f0f-9153-bf8ca592a159,
  abstract     = {<p>GPR151 is a G-protein coupled receptor for which the endogenous ligand remains unknown. In the nervous system of vertebrates, its expression is enriched in specific diencephalic structures, where the highest levels are observed in the habenular area. The habenula has been implicated in a range of different functions including behavioral flexibility, decision making, inhibitory control, and pain processing, which makes it a promising target for treating psychiatric and neurological disease. This study aimed to further characterize neurons expressing the Gpr151 gene, by tracing the afferent connectivity of this diencephalic cell population. Using pseudotyped rabies virus in a transgenic Gpr151-Cre mouse line, monosynaptic afferents of habenular and thalamic Gpr151-expressing neuronal populations could be visualized. The habenular and thalamic Gpr151 systems displayed both shared and distinct connectivity patterns. The habenular neurons primarily received input from basal forebrain structures, the bed nucleus of stria terminalis, the lateral preoptic area, the entopeduncular nucleus, and the lateral hypothalamic area. The Gpr151-expressing neurons in the paraventricular nucleus of the thalamus was primarily contacted by medial hypothalamic areas as well as the zona incerta and projected to specific forebrain areas such as the prelimbic cortex and the accumbens nucleus. Gpr151 mRNA was also detected at low levels in the lateral posterior thalamic nucleus which received input from areas associated with visual processing, including the superior colliculus, zona incerta, and the visual and retrosplenial cortices. Knowledge about the connectivity of Gpr151-expressing neurons will facilitate the interpretation of future functional studies of this receptor.</p>},
  author       = {Broms, Jonas and Grahm, Matilda and Haugegaard, Lea and Blom, Thomas and Meletis, Konstantinos and Tingström, Anders},
  issn         = {0021-9967},
  keyword      = {habenula,rabies,RRID: AB_10743815,RRID: AB_2571870,RRID: IMSR_JAX:000664,RRID: IMSR_JAX:024109,RRID: SCR_003070,thalamus},
  language     = {eng},
  month        = {10},
  number       = {15},
  pages        = {3227--3250},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Comparative Neurology},
  title        = {Monosynaptic retrograde tracing of neurons expressing the G-protein coupled receptor Gpr151 in the mouse brain},
  url          = {http://dx.doi.org/10.1002/cne.24273},
  volume       = {525},
  year         = {2017},
}