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Preparation of cultured human vascular cells

Olafsson, Ingvar H ; Vilhjalmsson, Dadi Th LU and Thormodsson, Finnbogi R (2012) In Methods in Molecular Biology 849. p.59-245
Abstract

Cerebral amyloid angiopathy (CAA) results from amyloid accumulation within arteries of the cerebral cortex and leptomeninges. This condition is age-related, especially prevalent in Alzheimer's disease (AD), and the main feature of certain hereditary disorders (i.e., HCHWA-I). The vascular smooth muscle cells (VSMCs) appear to play a vital role in the development of CAA, which makes them well suited as an experimental model to study the disease and screen for possible remedies. We describe two different methods for isolating and culturing human VSMCs. First, using the human umbilical cord as an easy source of robust cells, and secondly, using brain tissue that provides the proper cerebral VSMCs, but is more problematic to work with. The... (More)

Cerebral amyloid angiopathy (CAA) results from amyloid accumulation within arteries of the cerebral cortex and leptomeninges. This condition is age-related, especially prevalent in Alzheimer's disease (AD), and the main feature of certain hereditary disorders (i.e., HCHWA-I). The vascular smooth muscle cells (VSMCs) appear to play a vital role in the development of CAA, which makes them well suited as an experimental model to study the disease and screen for possible remedies. We describe two different methods for isolating and culturing human VSMCs. First, using the human umbilical cord as an easy source of robust cells, and secondly, using brain tissue that provides the proper cerebral VSMCs, but is more problematic to work with. The umbilical cord also provides human umbilical vascular endothelial cells (HUVECs), useful primary cells for vascular research. Finally, the maintenance, preservation, and characterization of the isolated vascular cells are described.

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Please use this url to cite or link to this publication:
author
; and
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
keywords
Cell Count, Cell Culture Techniques/methods, Cell Separation, Cryopreservation, Humans, Muscle, Smooth, Vascular/cytology, Umbilical Cord/cytology
host publication
Amyloid Proteins : Methods and Protocols - Methods and Protocols
series title
Methods in Molecular Biology
editor
Sigurdsson, EM
volume
849
pages
59 - 245
publisher
Humana Press
external identifiers
  • scopus:84864629824
  • pmid:22528095
ISSN
1940-6029
ISBN
978-1-61779-550-3
DOI
10.1007/978-1-61779-551-0_17
language
English
LU publication?
no
id
9bad794c-622e-47c4-892d-bedeeb5968d1
date added to LUP
2018-12-27 15:40:21
date last changed
2024-01-05 09:49:04
@inbook{9bad794c-622e-47c4-892d-bedeeb5968d1,
  abstract     = {{<p>Cerebral amyloid angiopathy (CAA) results from amyloid accumulation within arteries of the cerebral cortex and leptomeninges. This condition is age-related, especially prevalent in Alzheimer's disease (AD), and the main feature of certain hereditary disorders (i.e., HCHWA-I). The vascular smooth muscle cells (VSMCs) appear to play a vital role in the development of CAA, which makes them well suited as an experimental model to study the disease and screen for possible remedies. We describe two different methods for isolating and culturing human VSMCs. First, using the human umbilical cord as an easy source of robust cells, and secondly, using brain tissue that provides the proper cerebral VSMCs, but is more problematic to work with. The umbilical cord also provides human umbilical vascular endothelial cells (HUVECs), useful primary cells for vascular research. Finally, the maintenance, preservation, and characterization of the isolated vascular cells are described.</p>}},
  author       = {{Olafsson, Ingvar H and Vilhjalmsson, Dadi Th and Thormodsson, Finnbogi R}},
  booktitle    = {{Amyloid Proteins : Methods and Protocols}},
  editor       = {{Sigurdsson, EM}},
  isbn         = {{978-1-61779-550-3}},
  issn         = {{1940-6029}},
  keywords     = {{Cell Count; Cell Culture Techniques/methods; Cell Separation; Cryopreservation; Humans; Muscle, Smooth, Vascular/cytology; Umbilical Cord/cytology}},
  language     = {{eng}},
  pages        = {{59--245}},
  publisher    = {{Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Preparation of cultured human vascular cells}},
  url          = {{http://dx.doi.org/10.1007/978-1-61779-551-0_17}},
  doi          = {{10.1007/978-1-61779-551-0_17}},
  volume       = {{849}},
  year         = {{2012}},
}