The use of molecular methods in studies of avian haemosporidians
(2020) p.113-135- Abstract
- During the last two decades, molecular methods to study mitochondrial DNA sequence variation have become an important part in the studies of avian haemosporidians. Up until recently, these methods have primarily been used for identification of the parasites and for tentative phylogenetic reconstructions, allowing researchers not trained in traditional parasitology to compare data across the globe. However, with the introduction of genome and transcriptome sequencing, studies are emerging that go deeper into the genetics and molecular biology of the parasites. In this chapter, we describe and summarize the common methods used for genetic barcoding of the parasites and give an introduction of what to take into account when designing a... (More)
- During the last two decades, molecular methods to study mitochondrial DNA sequence variation have become an important part in the studies of avian haemosporidians. Up until recently, these methods have primarily been used for identification of the parasites and for tentative phylogenetic reconstructions, allowing researchers not trained in traditional parasitology to compare data across the globe. However, with the introduction of genome and transcriptome sequencing, studies are emerging that go deeper into the genetics and molecular biology of the parasites. In this chapter, we describe and summarize the common methods used for genetic barcoding of the parasites and give an introduction of what to take into account when designing a molecular study of avian haemosporidians. This chapter further discusses why nuclear genetic data are needed in order to answer several important ecological and evolutionary questions and which methods to use in order to overcome the obstacles of obtaining nuclear data of the parasites. Finally, this chapter highlights the challenges and opportunities that come with the use of molecular methods, such as how to study and interpret prevalence, the challenge of aborted developments, and how to obtain data for more robust phylogenies and population structure studies of the parasites. (Less)
- Abstract (Swedish)
- During the last two decades, molecular methods to study mitochondrial DNA sequence variation have become an important part in the studies of avian haemosporidians. Up until recently, these methods have primarily been used for identification of the parasites and for tentative phylogenetic reconstructions, allowing researchers not trained in traditional parasitology to compare data across the globe. However, with the introduction of genome and transcriptome sequencing, studies are emerging that go deeper into the genetics and molecular biology of the parasites. In this chapter, we describe and summarize the common methods used for genetic barcoding of the parasites and give an introduction of what to take into account when designing a... (More)
- During the last two decades, molecular methods to study mitochondrial DNA sequence variation have become an important part in the studies of avian haemosporidians. Up until recently, these methods have primarily been used for identification of the parasites and for tentative phylogenetic reconstructions, allowing researchers not trained in traditional parasitology to compare data across the globe. However, with the introduction of genome and transcriptome sequencing, studies are emerging that go deeper into the genetics and molecular biology of the parasites. In this chapter, we describe and summarize the common methods used for genetic barcoding of the parasites and give an introduction of what to take into account when designing a molecular study of avian haemosporidians. This chapter further discusses why nuclear genetic data are needed in order to answer several important ecological and evolutionary questions and which methods to use in order to overcome the obstacles of obtaining nuclear data of the parasites. Finally, this chapter highlights the challenges and opportunities that come with the use of molecular methods, such as how to study and interpret prevalence, the challenge of aborted developments, and how to obtain data for more robust phylogenies and population structure studies of the parasites. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/9bc8bb7b-232b-4283-bff2-a7b88c5916ae
- author
- Bensch, Staffan LU and Hellgren, Olof LU
- organization
- publishing date
- 2020
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- Avian Malaria and Related Parasites in the Tropics : Ecology, Evolution and Systematics - Ecology, Evolution and Systematics
- editor
- Santiago-Alarcon, Diego and Marzal, Alfonso
- pages
- 113 - 135
- publisher
- Springer
- external identifiers
-
- scopus:85094975507
- ISBN
- 978-3-030-51633-8
- 978-3-030-51632-1
- DOI
- 10.1007/978-3-030-51633-8_4
- language
- English
- LU publication?
- yes
- id
- 9bc8bb7b-232b-4283-bff2-a7b88c5916ae
- date added to LUP
- 2022-06-16 18:18:14
- date last changed
- 2024-04-29 16:33:25
@inbook{9bc8bb7b-232b-4283-bff2-a7b88c5916ae,
abstract = {{During the last two decades, molecular methods to study mitochondrial DNA sequence variation have become an important part in the studies of avian haemosporidians. Up until recently, these methods have primarily been used for identification of the parasites and for tentative phylogenetic reconstructions, allowing researchers not trained in traditional parasitology to compare data across the globe. However, with the introduction of genome and transcriptome sequencing, studies are emerging that go deeper into the genetics and molecular biology of the parasites. In this chapter, we describe and summarize the common methods used for genetic barcoding of the parasites and give an introduction of what to take into account when designing a molecular study of avian haemosporidians. This chapter further discusses why nuclear genetic data are needed in order to answer several important ecological and evolutionary questions and which methods to use in order to overcome the obstacles of obtaining nuclear data of the parasites. Finally, this chapter highlights the challenges and opportunities that come with the use of molecular methods, such as how to study and interpret prevalence, the challenge of aborted developments, and how to obtain data for more robust phylogenies and population structure studies of the parasites.}},
author = {{Bensch, Staffan and Hellgren, Olof}},
booktitle = {{Avian Malaria and Related Parasites in the Tropics : Ecology, Evolution and Systematics}},
editor = {{Santiago-Alarcon, Diego and Marzal, Alfonso}},
isbn = {{978-3-030-51633-8}},
language = {{eng}},
pages = {{113--135}},
publisher = {{Springer}},
title = {{The use of molecular methods in studies of avian haemosporidians}},
url = {{http://dx.doi.org/10.1007/978-3-030-51633-8_4}},
doi = {{10.1007/978-3-030-51633-8_4}},
year = {{2020}},
}