A general extracorporeal immunoadsorption method to increase tumor-to-tissue ratio
(1994) In Cancer 73(3 Suppl). p.1033-1037- Abstract
- The idea of applying extracorporeal immunoadsorption (ECIA) in radioimmunodiagnosis and radioimmunotherapy has been proposed previously. The authors here report on the development of new concept using a general method for ECIA based on biotinylated MoAb adsorbed on an avidin column. Athymic rats heterotransplanted with either human melanomas or human lung carcinoma were injected with iodine-125-labeled biotinylated 96.5 or L6 MoAb, respectively. At 24 or 48 hours after the injection, ECIA was performed by pumping blood through a hollow-fiber plasma filter. The separated plasma then was passed through an absorbent (avidin-agarose) column. The whole ECIA procedure lasted for 3 hours. By this ECIA method, the tumor-to-normal tissue ratios... (More)
- The idea of applying extracorporeal immunoadsorption (ECIA) in radioimmunodiagnosis and radioimmunotherapy has been proposed previously. The authors here report on the development of new concept using a general method for ECIA based on biotinylated MoAb adsorbed on an avidin column. Athymic rats heterotransplanted with either human melanomas or human lung carcinoma were injected with iodine-125-labeled biotinylated 96.5 or L6 MoAb, respectively. At 24 or 48 hours after the injection, ECIA was performed by pumping blood through a hollow-fiber plasma filter. The separated plasma then was passed through an absorbent (avidin-agarose) column. The whole ECIA procedure lasted for 3 hours. By this ECIA method, the tumor-to-normal tissue ratios were increased in various tissues (i.e., radiosensitive and blood rich organs) by a factor of four to five. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1107982
- author
- Strand, Sven-Erik LU ; Norrgren, Kristina LU ; Garkavij, Michael LU ; Lindgren, L ; Nilsson, Rune LU ; Sjogren, H O and Tennvall, Jan LU
- organization
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cancer
- volume
- 73
- issue
- 3 Suppl
- pages
- 1033 - 1037
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:8306245
- scopus:0028125842
- ISSN
- 1097-0142
- language
- English
- LU publication?
- yes
- id
- 9bd788d6-42ef-42d2-8cc2-5ee23905d022 (old id 1107982)
- date added to LUP
- 2016-04-01 12:23:41
- date last changed
- 2021-01-03 06:36:09
@article{9bd788d6-42ef-42d2-8cc2-5ee23905d022, abstract = {{The idea of applying extracorporeal immunoadsorption (ECIA) in radioimmunodiagnosis and radioimmunotherapy has been proposed previously. The authors here report on the development of new concept using a general method for ECIA based on biotinylated MoAb adsorbed on an avidin column. Athymic rats heterotransplanted with either human melanomas or human lung carcinoma were injected with iodine-125-labeled biotinylated 96.5 or L6 MoAb, respectively. At 24 or 48 hours after the injection, ECIA was performed by pumping blood through a hollow-fiber plasma filter. The separated plasma then was passed through an absorbent (avidin-agarose) column. The whole ECIA procedure lasted for 3 hours. By this ECIA method, the tumor-to-normal tissue ratios were increased in various tissues (i.e., radiosensitive and blood rich organs) by a factor of four to five.}}, author = {{Strand, Sven-Erik and Norrgren, Kristina and Garkavij, Michael and Lindgren, L and Nilsson, Rune and Sjogren, H O and Tennvall, Jan}}, issn = {{1097-0142}}, language = {{eng}}, number = {{3 Suppl}}, pages = {{1033--1037}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Cancer}}, title = {{A general extracorporeal immunoadsorption method to increase tumor-to-tissue ratio}}, volume = {{73}}, year = {{1994}}, }