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Higher expression of WNT5A protein in oral squamous cell carcinoma compared with dysplasia and oral mucosa with a normal appearance

Prgomet, Zdenka LU ; Andersson, Tommy LU and Lindberg, Pia LU (2017) In European Journal of Oral Sciences1995-01-01+01:00 125(4). p.237-246
Abstract

WNT5A is a secreted signaling protein that promotes migration and invasion of oral squamous cell carcinoma (OSCC) cells through activation of non-canonical WNT signaling. Here, we examined expression of WNT5A, β-catenin, and E-cadherin by immunohistochemistry in 21 human diagnostic incision biopsies that each had regions of oral mucosa with a normal appearance adjacent to the affected tissue, dysplasia, and OSCC. We also investigated the effect of recombinant WNT5A (rWNT5A) on expression of the cell-adhesion proteins E-cadherin and β-catenin by western blot analysis. No expression of WNT5A protein was present in oral mucosa with a normal appearance or in mild grade dysplasia. However, expression of WNT5A increased along with increasing... (More)

WNT5A is a secreted signaling protein that promotes migration and invasion of oral squamous cell carcinoma (OSCC) cells through activation of non-canonical WNT signaling. Here, we examined expression of WNT5A, β-catenin, and E-cadherin by immunohistochemistry in 21 human diagnostic incision biopsies that each had regions of oral mucosa with a normal appearance adjacent to the affected tissue, dysplasia, and OSCC. We also investigated the effect of recombinant WNT5A (rWNT5A) on expression of the cell-adhesion proteins E-cadherin and β-catenin by western blot analysis. No expression of WNT5A protein was present in oral mucosa with a normal appearance or in mild grade dysplasia. However, expression of WNT5A increased along with increasing grade of dysplasia, and the highest expression was detected in OSCCs. Expression of membranous β-catenin and of E-cadherin was lower, whereas expression of cytoplasmic β-catenin was higher, in OSCCs than in non-cancerous regions. However, there was no correlation between expression of WNT5A and expression of either β-catenin or E-cadherin. Furthermore, treatment of OSCC cells with rWNT5A had no effect on the expression of β-catenin or E-cadherin. Taken together with previous results, we conclude that WNT5A influences the progression of OSCC without affecting the canonical WNT/β-catenin pathway and without down-regulating E-cadherin. WNT5A may have potential as a biological marker for malignant transformation of dysplasia to OSCC.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
E-cadherin, Immunohistochemistry, Oral cancer, WNT5A, β-catenin
in
European Journal of Oral Sciences1995-01-01+01:00
volume
125
issue
4
pages
237 - 246
publisher
Wiley-Blackwell
external identifiers
  • scopus:85020442819
  • wos:000406976900001
ISSN
0909-8836
DOI
10.1111/eos.12352
language
English
LU publication?
yes
id
9c3a8fc1-37f2-49a1-be80-2ef01fce991a
date added to LUP
2017-06-30 08:23:53
date last changed
2018-01-07 12:09:58
@article{9c3a8fc1-37f2-49a1-be80-2ef01fce991a,
  abstract     = {<p>WNT5A is a secreted signaling protein that promotes migration and invasion of oral squamous cell carcinoma (OSCC) cells through activation of non-canonical WNT signaling. Here, we examined expression of WNT5A, β-catenin, and E-cadherin by immunohistochemistry in 21 human diagnostic incision biopsies that each had regions of oral mucosa with a normal appearance adjacent to the affected tissue, dysplasia, and OSCC. We also investigated the effect of recombinant WNT5A (rWNT5A) on expression of the cell-adhesion proteins E-cadherin and β-catenin by western blot analysis. No expression of WNT5A protein was present in oral mucosa with a normal appearance or in mild grade dysplasia. However, expression of WNT5A increased along with increasing grade of dysplasia, and the highest expression was detected in OSCCs. Expression of membranous β-catenin and of E-cadherin was lower, whereas expression of cytoplasmic β-catenin was higher, in OSCCs than in non-cancerous regions. However, there was no correlation between expression of WNT5A and expression of either β-catenin or E-cadherin. Furthermore, treatment of OSCC cells with rWNT5A had no effect on the expression of β-catenin or E-cadherin. Taken together with previous results, we conclude that WNT5A influences the progression of OSCC without affecting the canonical WNT/β-catenin pathway and without down-regulating E-cadherin. WNT5A may have potential as a biological marker for malignant transformation of dysplasia to OSCC.</p>},
  author       = {Prgomet, Zdenka and Andersson, Tommy and Lindberg, Pia},
  issn         = {0909-8836},
  keyword      = {E-cadherin,Immunohistochemistry,Oral cancer,WNT5A,β-catenin},
  language     = {eng},
  month        = {06},
  number       = {4},
  pages        = {237--246},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Oral Sciences1995-01-01+01:00},
  title        = {Higher expression of WNT5A protein in oral squamous cell carcinoma compared with dysplasia and oral mucosa with a normal appearance},
  url          = {http://dx.doi.org/10.1111/eos.12352},
  volume       = {125},
  year         = {2017},
}