Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Higher expression of WNT5A protein in oral squamous cell carcinoma compared with dysplasia and oral mucosa with a normal appearance

Prgomet, Zdenka LU ; Andersson, Tommy LU and Lindberg, Pia LU (2017) In European Journal of Oral Sciences 125(4). p.237-246
Abstract

WNT5A is a secreted signaling protein that promotes migration and invasion of oral squamous cell carcinoma (OSCC) cells through activation of non-canonical WNT signaling. Here, we examined expression of WNT5A, β-catenin, and E-cadherin by immunohistochemistry in 21 human diagnostic incision biopsies that each had regions of oral mucosa with a normal appearance adjacent to the affected tissue, dysplasia, and OSCC. We also investigated the effect of recombinant WNT5A (rWNT5A) on expression of the cell-adhesion proteins E-cadherin and β-catenin by western blot analysis. No expression of WNT5A protein was present in oral mucosa with a normal appearance or in mild grade dysplasia. However, expression of WNT5A increased along with increasing... (More)

WNT5A is a secreted signaling protein that promotes migration and invasion of oral squamous cell carcinoma (OSCC) cells through activation of non-canonical WNT signaling. Here, we examined expression of WNT5A, β-catenin, and E-cadherin by immunohistochemistry in 21 human diagnostic incision biopsies that each had regions of oral mucosa with a normal appearance adjacent to the affected tissue, dysplasia, and OSCC. We also investigated the effect of recombinant WNT5A (rWNT5A) on expression of the cell-adhesion proteins E-cadherin and β-catenin by western blot analysis. No expression of WNT5A protein was present in oral mucosa with a normal appearance or in mild grade dysplasia. However, expression of WNT5A increased along with increasing grade of dysplasia, and the highest expression was detected in OSCCs. Expression of membranous β-catenin and of E-cadherin was lower, whereas expression of cytoplasmic β-catenin was higher, in OSCCs than in non-cancerous regions. However, there was no correlation between expression of WNT5A and expression of either β-catenin or E-cadherin. Furthermore, treatment of OSCC cells with rWNT5A had no effect on the expression of β-catenin or E-cadherin. Taken together with previous results, we conclude that WNT5A influences the progression of OSCC without affecting the canonical WNT/β-catenin pathway and without down-regulating E-cadherin. WNT5A may have potential as a biological marker for malignant transformation of dysplasia to OSCC.

(Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
E-cadherin, Immunohistochemistry, Oral cancer, WNT5A, β-catenin
in
European Journal of Oral Sciences
volume
125
issue
4
pages
237 - 246
publisher
Wiley-Blackwell
external identifiers
  • scopus:85020442819
  • wos:000406976900001
  • pmid:28603941
ISSN
0909-8836
DOI
10.1111/eos.12352
language
English
LU publication?
yes
id
9c3a8fc1-37f2-49a1-be80-2ef01fce991a
date added to LUP
2017-06-30 08:23:53
date last changed
2024-04-14 13:29:15
@article{9c3a8fc1-37f2-49a1-be80-2ef01fce991a,
  abstract     = {{<p>WNT5A is a secreted signaling protein that promotes migration and invasion of oral squamous cell carcinoma (OSCC) cells through activation of non-canonical WNT signaling. Here, we examined expression of WNT5A, β-catenin, and E-cadherin by immunohistochemistry in 21 human diagnostic incision biopsies that each had regions of oral mucosa with a normal appearance adjacent to the affected tissue, dysplasia, and OSCC. We also investigated the effect of recombinant WNT5A (rWNT5A) on expression of the cell-adhesion proteins E-cadherin and β-catenin by western blot analysis. No expression of WNT5A protein was present in oral mucosa with a normal appearance or in mild grade dysplasia. However, expression of WNT5A increased along with increasing grade of dysplasia, and the highest expression was detected in OSCCs. Expression of membranous β-catenin and of E-cadherin was lower, whereas expression of cytoplasmic β-catenin was higher, in OSCCs than in non-cancerous regions. However, there was no correlation between expression of WNT5A and expression of either β-catenin or E-cadherin. Furthermore, treatment of OSCC cells with rWNT5A had no effect on the expression of β-catenin or E-cadherin. Taken together with previous results, we conclude that WNT5A influences the progression of OSCC without affecting the canonical WNT/β-catenin pathway and without down-regulating E-cadherin. WNT5A may have potential as a biological marker for malignant transformation of dysplasia to OSCC.</p>}},
  author       = {{Prgomet, Zdenka and Andersson, Tommy and Lindberg, Pia}},
  issn         = {{0909-8836}},
  keywords     = {{E-cadherin; Immunohistochemistry; Oral cancer; WNT5A; β-catenin}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{4}},
  pages        = {{237--246}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{European Journal of Oral Sciences}},
  title        = {{Higher expression of WNT5A protein in oral squamous cell carcinoma compared with dysplasia and oral mucosa with a normal appearance}},
  url          = {{http://dx.doi.org/10.1111/eos.12352}},
  doi          = {{10.1111/eos.12352}},
  volume       = {{125}},
  year         = {{2017}},
}