Higher expression of WNT5A protein in oral squamous cell carcinoma compared with dysplasia and oral mucosa with a normal appearance
(2017) In European Journal of Oral Sciences 125(4). p.237-246- Abstract
WNT5A is a secreted signaling protein that promotes migration and invasion of oral squamous cell carcinoma (OSCC) cells through activation of non-canonical WNT signaling. Here, we examined expression of WNT5A, β-catenin, and E-cadherin by immunohistochemistry in 21 human diagnostic incision biopsies that each had regions of oral mucosa with a normal appearance adjacent to the affected tissue, dysplasia, and OSCC. We also investigated the effect of recombinant WNT5A (rWNT5A) on expression of the cell-adhesion proteins E-cadherin and β-catenin by western blot analysis. No expression of WNT5A protein was present in oral mucosa with a normal appearance or in mild grade dysplasia. However, expression of WNT5A increased along with increasing... (More)
WNT5A is a secreted signaling protein that promotes migration and invasion of oral squamous cell carcinoma (OSCC) cells through activation of non-canonical WNT signaling. Here, we examined expression of WNT5A, β-catenin, and E-cadherin by immunohistochemistry in 21 human diagnostic incision biopsies that each had regions of oral mucosa with a normal appearance adjacent to the affected tissue, dysplasia, and OSCC. We also investigated the effect of recombinant WNT5A (rWNT5A) on expression of the cell-adhesion proteins E-cadherin and β-catenin by western blot analysis. No expression of WNT5A protein was present in oral mucosa with a normal appearance or in mild grade dysplasia. However, expression of WNT5A increased along with increasing grade of dysplasia, and the highest expression was detected in OSCCs. Expression of membranous β-catenin and of E-cadherin was lower, whereas expression of cytoplasmic β-catenin was higher, in OSCCs than in non-cancerous regions. However, there was no correlation between expression of WNT5A and expression of either β-catenin or E-cadherin. Furthermore, treatment of OSCC cells with rWNT5A had no effect on the expression of β-catenin or E-cadherin. Taken together with previous results, we conclude that WNT5A influences the progression of OSCC without affecting the canonical WNT/β-catenin pathway and without down-regulating E-cadherin. WNT5A may have potential as a biological marker for malignant transformation of dysplasia to OSCC.
(Less)
- author
- Prgomet, Zdenka LU ; Andersson, Tommy LU and Lindberg, Pia LU
- organization
- publishing date
- 2017-06-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- E-cadherin, Immunohistochemistry, Oral cancer, WNT5A, β-catenin
- in
- European Journal of Oral Sciences
- volume
- 125
- issue
- 4
- pages
- 237 - 246
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:85020442819
- wos:000406976900001
- pmid:28603941
- ISSN
- 0909-8836
- DOI
- 10.1111/eos.12352
- language
- English
- LU publication?
- yes
- id
- 9c3a8fc1-37f2-49a1-be80-2ef01fce991a
- date added to LUP
- 2017-06-30 08:23:53
- date last changed
- 2025-01-21 20:03:50
@article{9c3a8fc1-37f2-49a1-be80-2ef01fce991a,
abstract = {{<p>WNT5A is a secreted signaling protein that promotes migration and invasion of oral squamous cell carcinoma (OSCC) cells through activation of non-canonical WNT signaling. Here, we examined expression of WNT5A, β-catenin, and E-cadherin by immunohistochemistry in 21 human diagnostic incision biopsies that each had regions of oral mucosa with a normal appearance adjacent to the affected tissue, dysplasia, and OSCC. We also investigated the effect of recombinant WNT5A (rWNT5A) on expression of the cell-adhesion proteins E-cadherin and β-catenin by western blot analysis. No expression of WNT5A protein was present in oral mucosa with a normal appearance or in mild grade dysplasia. However, expression of WNT5A increased along with increasing grade of dysplasia, and the highest expression was detected in OSCCs. Expression of membranous β-catenin and of E-cadherin was lower, whereas expression of cytoplasmic β-catenin was higher, in OSCCs than in non-cancerous regions. However, there was no correlation between expression of WNT5A and expression of either β-catenin or E-cadherin. Furthermore, treatment of OSCC cells with rWNT5A had no effect on the expression of β-catenin or E-cadherin. Taken together with previous results, we conclude that WNT5A influences the progression of OSCC without affecting the canonical WNT/β-catenin pathway and without down-regulating E-cadherin. WNT5A may have potential as a biological marker for malignant transformation of dysplasia to OSCC.</p>}},
author = {{Prgomet, Zdenka and Andersson, Tommy and Lindberg, Pia}},
issn = {{0909-8836}},
keywords = {{E-cadherin; Immunohistochemistry; Oral cancer; WNT5A; β-catenin}},
language = {{eng}},
month = {{06}},
number = {{4}},
pages = {{237--246}},
publisher = {{Wiley-Blackwell}},
series = {{European Journal of Oral Sciences}},
title = {{Higher expression of WNT5A protein in oral squamous cell carcinoma compared with dysplasia and oral mucosa with a normal appearance}},
url = {{http://dx.doi.org/10.1111/eos.12352}},
doi = {{10.1111/eos.12352}},
volume = {{125}},
year = {{2017}},
}