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Supravital dithizone staining in the isolation of human and rat pancreatic islets

Hansen, W. A. ; Christie, M. R. ; Kahn, R. LU ; Norgaard, A. ; Abel, I. ; Petersen, A. M. ; Jorgensen, D. W. ; Baekkeskov, S. ; Nielsen, J. H. and Lernmark, A. LU orcid , et al. (1989) In Diabetes Research 10(2). p.53-57
Abstract

Dithizone, a zinc chelating agent, is known to selectively stain the islets of Langerhans in the pancreas. In the present study, we have used this stain to aid the identification of islets in material obtained by collagenase digestion of human pancreas. Islets were shown to rapidly and reversibly stain red on incubation with dithizone solution. Tissue selected on the basis of dithizone staining was shown to contain insulin-positive cells and to accumulate insulin in the medium during a subsequent period in tissue culture. Experiments with rat islets indicated that the dithizone treatment had no effect on insulin release in tissue culture, on acute responses to stimulatory glucose concentrations or on the insulin content of cells. These... (More)

Dithizone, a zinc chelating agent, is known to selectively stain the islets of Langerhans in the pancreas. In the present study, we have used this stain to aid the identification of islets in material obtained by collagenase digestion of human pancreas. Islets were shown to rapidly and reversibly stain red on incubation with dithizone solution. Tissue selected on the basis of dithizone staining was shown to contain insulin-positive cells and to accumulate insulin in the medium during a subsequent period in tissue culture. Experiments with rat islets indicated that the dithizone treatment had no effect on insulin release in tissue culture, on acute responses to stimulatory glucose concentrations or on the insulin content of cells. These results suggest that dithizone staining can assist in the identification of islets from the human pancreas and may prove to be a useful tool in developing techniques for the large scale isolation of functionally intact human islets.

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publishing date
type
Contribution to journal
publication status
published
in
Diabetes Research
volume
10
issue
2
pages
5 pages
publisher
Teviot-Kimpton
external identifiers
  • scopus:0024464239
  • pmid:2545405
ISSN
0265-5985
language
English
LU publication?
no
id
9c64a256-f064-4b42-8749-546b19541237
date added to LUP
2019-09-11 09:57:32
date last changed
2024-03-13 08:18:02
@article{9c64a256-f064-4b42-8749-546b19541237,
  abstract     = {{<p>Dithizone, a zinc chelating agent, is known to selectively stain the islets of Langerhans in the pancreas. In the present study, we have used this stain to aid the identification of islets in material obtained by collagenase digestion of human pancreas. Islets were shown to rapidly and reversibly stain red on incubation with dithizone solution. Tissue selected on the basis of dithizone staining was shown to contain insulin-positive cells and to accumulate insulin in the medium during a subsequent period in tissue culture. Experiments with rat islets indicated that the dithizone treatment had no effect on insulin release in tissue culture, on acute responses to stimulatory glucose concentrations or on the insulin content of cells. These results suggest that dithizone staining can assist in the identification of islets from the human pancreas and may prove to be a useful tool in developing techniques for the large scale isolation of functionally intact human islets.</p>}},
  author       = {{Hansen, W. A. and Christie, M. R. and Kahn, R. and Norgaard, A. and Abel, I. and Petersen, A. M. and Jorgensen, D. W. and Baekkeskov, S. and Nielsen, J. H. and Lernmark, A. and Egeberg, J. and Richter-Olesen, H. and Grainger, T. and Kristensen, J. K. and Brynitz, S. and Bilde, T.}},
  issn         = {{0265-5985}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{2}},
  pages        = {{53--57}},
  publisher    = {{Teviot-Kimpton}},
  series       = {{Diabetes Research}},
  title        = {{Supravital dithizone staining in the isolation of human and rat pancreatic islets}},
  volume       = {{10}},
  year         = {{1989}},
}