Microbiosensor based on an integrated thermopile
(1994) In Analytica Chimica Acta 299(2). p.165-170- Abstract
A microbiosensor based on an integrated thermopile was designed and fabricated on a quartz chip. The thermopile, which was manufactured by doping boron in polysilicon together with aluminium, provided a potential output of ca. 2 mV K. A silicone rubber membrane was used to form and seal the microchannel. The total column volume was 20 μl. Glucose oxidase and catalase were co-immobilized on spherical CPG beads (controlled pore glass) and in turn charged into the microchannel. Using 1 μl sample volumes, a linear range of 2 to 25 mM glucose was obtained using a flow rate of 105 μl min. The relative standard deviation for 100 glucose samples (10 mM) was 5%.
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https://lup.lub.lu.se/record/9c683ae2-3141-4fff-9535-86b92a8e70a9
- author
- Xie, Bin LU ; Mecklenburg, Michael ; Danielsson, Bengt LU ; Öhman, Ove and Winquist, Fredrik
- organization
- publishing date
- 1994-12-30
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biosensors, Flow injection, Glucose, Integrated thermopile, Micromachining, Thermal biosensor
- in
- Analytica Chimica Acta
- volume
- 299
- issue
- 2
- pages
- 6 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0028607945
- ISSN
- 0003-2670
- DOI
- 10.1016/0003-2670(94)00346-7
- language
- English
- LU publication?
- yes
- id
- 9c683ae2-3141-4fff-9535-86b92a8e70a9
- date added to LUP
- 2021-10-27 15:58:39
- date last changed
- 2021-11-01 11:44:32
@article{9c683ae2-3141-4fff-9535-86b92a8e70a9, abstract = {{<p>A microbiosensor based on an integrated thermopile was designed and fabricated on a quartz chip. The thermopile, which was manufactured by doping boron in polysilicon together with aluminium, provided a potential output of ca. 2 mV K. A silicone rubber membrane was used to form and seal the microchannel. The total column volume was 20 μl. Glucose oxidase and catalase were co-immobilized on spherical CPG beads (controlled pore glass) and in turn charged into the microchannel. Using 1 μl sample volumes, a linear range of 2 to 25 mM glucose was obtained using a flow rate of 105 μl min. The relative standard deviation for 100 glucose samples (10 mM) was 5%.</p>}}, author = {{Xie, Bin and Mecklenburg, Michael and Danielsson, Bengt and Öhman, Ove and Winquist, Fredrik}}, issn = {{0003-2670}}, keywords = {{Biosensors; Flow injection; Glucose; Integrated thermopile; Micromachining; Thermal biosensor}}, language = {{eng}}, month = {{12}}, number = {{2}}, pages = {{165--170}}, publisher = {{Elsevier}}, series = {{Analytica Chimica Acta}}, title = {{Microbiosensor based on an integrated thermopile}}, url = {{http://dx.doi.org/10.1016/0003-2670(94)00346-7}}, doi = {{10.1016/0003-2670(94)00346-7}}, volume = {{299}}, year = {{1994}}, }