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Microbiosensor based on an integrated thermopile

Xie, Bin LU ; Mecklenburg, Michael ; Danielsson, Bengt LU ; Öhman, Ove and Winquist, Fredrik (1994) In Analytica Chimica Acta 299(2). p.165-170
Abstract

A microbiosensor based on an integrated thermopile was designed and fabricated on a quartz chip. The thermopile, which was manufactured by doping boron in polysilicon together with aluminium, provided a potential output of ca. 2 mV K. A silicone rubber membrane was used to form and seal the microchannel. The total column volume was 20 μl. Glucose oxidase and catalase were co-immobilized on spherical CPG beads (controlled pore glass) and in turn charged into the microchannel. Using 1 μl sample volumes, a linear range of 2 to 25 mM glucose was obtained using a flow rate of 105 μl min. The relative standard deviation for 100 glucose samples (10 mM) was 5%.

Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biosensors, Flow injection, Glucose, Integrated thermopile, Micromachining, Thermal biosensor
in
Analytica Chimica Acta
volume
299
issue
2
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:0028607945
ISSN
0003-2670
DOI
10.1016/0003-2670(94)00346-7
language
English
LU publication?
yes
id
9c683ae2-3141-4fff-9535-86b92a8e70a9
date added to LUP
2021-10-27 15:58:39
date last changed
2021-11-01 11:44:32
@article{9c683ae2-3141-4fff-9535-86b92a8e70a9,
  abstract     = {{<p>A microbiosensor based on an integrated thermopile was designed and fabricated on a quartz chip. The thermopile, which was manufactured by doping boron in polysilicon together with aluminium, provided a potential output of ca. 2 mV K. A silicone rubber membrane was used to form and seal the microchannel. The total column volume was 20 μl. Glucose oxidase and catalase were co-immobilized on spherical CPG beads (controlled pore glass) and in turn charged into the microchannel. Using 1 μl sample volumes, a linear range of 2 to 25 mM glucose was obtained using a flow rate of 105 μl min. The relative standard deviation for 100 glucose samples (10 mM) was 5%.</p>}},
  author       = {{Xie, Bin and Mecklenburg, Michael and Danielsson, Bengt and Öhman, Ove and Winquist, Fredrik}},
  issn         = {{0003-2670}},
  keywords     = {{Biosensors; Flow injection; Glucose; Integrated thermopile; Micromachining; Thermal biosensor}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{2}},
  pages        = {{165--170}},
  publisher    = {{Elsevier}},
  series       = {{Analytica Chimica Acta}},
  title        = {{Microbiosensor based on an integrated thermopile}},
  url          = {{http://dx.doi.org/10.1016/0003-2670(94)00346-7}},
  doi          = {{10.1016/0003-2670(94)00346-7}},
  volume       = {{299}},
  year         = {{1994}},
}