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Carbon Nanotube Matrix for Highly Sensitive Biosensors to Detect Pancreatic Cancer Biomarker CA19-9

Thapa, Anshu; Soares, Andrey Coatrini; Soares, Juliana Coatrini; Awan, Iram Taj; Volpati, Diogo LU ; Melendez, Matias Eliseo; Fregnani, José Humberto Tavares Guerreiro; Carvalho, André Lopes and Oliveira, Osvaldo N. (2017) In ACS Applied Materials and Interfaces 9(31). p.25878-25886
Abstract

Biosensors fabricated with nanomaterials promise faster, cheaper, and more efficient alternatives to traditional, often bulky devices for early cancer diagnosis. In this study, we fabricated a thin film sensing unit on interdigitated gold electrodes combining polyethyleneimine and carbon nanotubes in a layer by layer fashion, onto which antibodies anti-CA19-9 were adsorbed with a supporting layer of N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide solution. By use of impedance spectroscopy, the pancreatic cancer biomarker CA19-9 was detected in a buffer with limit of detection of 0.35 U/mL. This high sensitivity allowed for distinction between samples of blood serum from patients with distinct probabilities to... (More)

Biosensors fabricated with nanomaterials promise faster, cheaper, and more efficient alternatives to traditional, often bulky devices for early cancer diagnosis. In this study, we fabricated a thin film sensing unit on interdigitated gold electrodes combining polyethyleneimine and carbon nanotubes in a layer by layer fashion, onto which antibodies anti-CA19-9 were adsorbed with a supporting layer of N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide solution. By use of impedance spectroscopy, the pancreatic cancer biomarker CA19-9 was detected in a buffer with limit of detection of 0.35 U/mL. This high sensitivity allowed for distinction between samples of blood serum from patients with distinct probabilities to develop pancreatic cancer. The selectivity of the biosensor was confirmed in subsidiary experiments with HT-29 and SW-620 cell lines and possible interferents, e.g., p53 protein, ascorbic acid, and glucose, where significant changes in capacitance could only be measured with HT-29 that contained the CA19-9 biomarker. Chemisorption of CA19-9 molecules onto the layer of anti-CA19-9 antibodies was the mechanism responsible for sensing while electrostatic interactions drove the adsorption of carbon nanotubes, according to polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). The adsorption behavior was successfully described by the Langmuir-Freundlich isotherm.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
biomarkers, cancer, carbon nanotubes, immunosensors, impedance spectroscopy
in
ACS Applied Materials and Interfaces
volume
9
issue
31
pages
9 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:85027237112
ISSN
1944-8244
DOI
10.1021/acsami.7b07384
language
English
LU publication?
no
id
9ca4b021-4be2-42e0-a0d6-e27fc6027188
date added to LUP
2019-05-17 14:28:04
date last changed
2019-11-05 05:18:57
@article{9ca4b021-4be2-42e0-a0d6-e27fc6027188,
  abstract     = {<p>Biosensors fabricated with nanomaterials promise faster, cheaper, and more efficient alternatives to traditional, often bulky devices for early cancer diagnosis. In this study, we fabricated a thin film sensing unit on interdigitated gold electrodes combining polyethyleneimine and carbon nanotubes in a layer by layer fashion, onto which antibodies anti-CA19-9 were adsorbed with a supporting layer of N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide solution. By use of impedance spectroscopy, the pancreatic cancer biomarker CA19-9 was detected in a buffer with limit of detection of 0.35 U/mL. This high sensitivity allowed for distinction between samples of blood serum from patients with distinct probabilities to develop pancreatic cancer. The selectivity of the biosensor was confirmed in subsidiary experiments with HT-29 and SW-620 cell lines and possible interferents, e.g., p53 protein, ascorbic acid, and glucose, where significant changes in capacitance could only be measured with HT-29 that contained the CA19-9 biomarker. Chemisorption of CA19-9 molecules onto the layer of anti-CA19-9 antibodies was the mechanism responsible for sensing while electrostatic interactions drove the adsorption of carbon nanotubes, according to polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). The adsorption behavior was successfully described by the Langmuir-Freundlich isotherm.</p>},
  author       = {Thapa, Anshu and Soares, Andrey Coatrini and Soares, Juliana Coatrini and Awan, Iram Taj and Volpati, Diogo and Melendez, Matias Eliseo and Fregnani, José Humberto Tavares Guerreiro and Carvalho, André Lopes and Oliveira, Osvaldo N.},
  issn         = {1944-8244},
  keyword      = {biomarkers,cancer,carbon nanotubes,immunosensors,impedance spectroscopy},
  language     = {eng},
  month        = {08},
  number       = {31},
  pages        = {25878--25886},
  publisher    = {The American Chemical Society (ACS)},
  series       = {ACS Applied Materials and Interfaces},
  title        = {Carbon Nanotube Matrix for Highly Sensitive Biosensors to Detect Pancreatic Cancer Biomarker CA19-9},
  url          = {http://dx.doi.org/10.1021/acsami.7b07384},
  volume       = {9},
  year         = {2017},
}