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The hemX gene of the Bacillus subtilis hemAXCDBL operon encodes a membrane protein, negatively affecting the steady-state cellular concentration of HemA (glutamyl-tRNA reductase).

Schröder, Ingrid LU ; Johansson, Per; Rutberg, Lars and Hederstedt, Lars LU (1994) In Microbiology 140.
Abstract
The Bacillus subtilis hemAXCDBL operon encodes enzymes for the biosynthesis of uroporphyrinogen III from glutamyl-tRNA. The function of the hemX gene product was studied in this work. The deduced amino acid sequence suggests HemX to be an integral 32 kDa membrane protein. This was confirmed by experiments using Escherichia coli minicells and hemX-phoA gene fusions. Deletion of the hemX gene from the Bacillus subtilis chromosome demonstrated that this gene is net required for haem synthesis. However, the deletion strain was found to overexpress the hemA gene product, glutamyl-tRNA reductase. A combination of results obtained with B. subtilis hemA and hemX in Escherichia coli and Bacillus subtilis shows that HemX negatively affects the... (More)
The Bacillus subtilis hemAXCDBL operon encodes enzymes for the biosynthesis of uroporphyrinogen III from glutamyl-tRNA. The function of the hemX gene product was studied in this work. The deduced amino acid sequence suggests HemX to be an integral 32 kDa membrane protein. This was confirmed by experiments using Escherichia coli minicells and hemX-phoA gene fusions. Deletion of the hemX gene from the Bacillus subtilis chromosome demonstrated that this gene is net required for haem synthesis. However, the deletion strain was found to overexpress the hemA gene product, glutamyl-tRNA reductase. A combination of results obtained with B. subtilis hemA and hemX in Escherichia coli and Bacillus subtilis shows that HemX negatively affects the steady-state cellular concentration of HemA protein. The mechanism by which HemX affects the HemA concentration is unclear. (Less)
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Contribution to journal
publication status
published
subject
in
Microbiology
volume
140
publisher
MAIK Nauka/Interperiodica
ISSN
1465-2080
DOI
10.1099/00221287-140-4-731
language
English
LU publication?
yes
id
9d039d43-d025-42ee-85ab-4c82e76d8cda
date added to LUP
2017-07-18 10:18:26
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2017-08-15 16:30:00
@article{9d039d43-d025-42ee-85ab-4c82e76d8cda,
  abstract     = {The Bacillus subtilis hemAXCDBL operon encodes enzymes for the biosynthesis of uroporphyrinogen III from glutamyl-tRNA. The function of the hemX gene product was studied in this work. The deduced amino acid sequence suggests HemX to be an integral 32 kDa membrane protein. This was confirmed by experiments using Escherichia coli minicells and hemX-phoA gene fusions. Deletion of the hemX gene from the Bacillus subtilis chromosome demonstrated that this gene is net required for haem synthesis. However, the deletion strain was found to overexpress the hemA gene product, glutamyl-tRNA reductase. A combination of results obtained with B. subtilis hemA and hemX in Escherichia coli and Bacillus subtilis shows that HemX negatively affects the steady-state cellular concentration of HemA protein. The mechanism by which HemX affects the HemA concentration is unclear.},
  author       = {Schröder, Ingrid and Johansson, Per and Rutberg, Lars and Hederstedt, Lars},
  issn         = {1465-2080},
  language     = {eng},
  publisher    = {MAIK Nauka/Interperiodica},
  series       = {Microbiology},
  title        = {The hemX gene of the <em>Bacillus subtilis hemAXCDBL</em> operon encodes a membrane protein, negatively affecting the steady-state cellular concentration of HemA (glutamyl-tRNA reductase).},
  url          = {http://dx.doi.org/ 10.1099/00221287-140-4-731 },
  volume       = {140},
  year         = {1994},
}