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The transcription factor MYB29 is a regulator of alternative oxidase1a

Zhang, Xinhua; Ivanova, Aneta; Vandepoele, Klaas; Radomiljac, Jordan; Van De Velde, Jan; Berkowitz, Oliver; Willems, Patrick; Xu, Yue; Ng, Sophia and Van Aken, Olivier LU , et al. (2017) In Plant Physiology 173(3). p.1825-1843
Abstract

Plants sense and integrate a variety of signals from the environment through different interacting signal transduction pathways that involve hormones and signaling molecules. Using ALTERNATIVE OXIDASE1a (AOX1a) gene expression as a model system of retrograde or stress signaling between mitochondria and the nucleus, MYB DOMAIN PROTEIN29 (MYB29) was identified as a negative regulator (regulator of alternative oxidase1a 7 [rao7] mutant) in a genetic screen of Arabidopsis (Arabidopsis thaliana). rao7/ myb29 mutants have increased levels of AOX1a transcript and protein compared to wild type after induction with antimycin A. A variety of genes previously associated with the mitochondrial stress response also display enhanced transcript... (More)

Plants sense and integrate a variety of signals from the environment through different interacting signal transduction pathways that involve hormones and signaling molecules. Using ALTERNATIVE OXIDASE1a (AOX1a) gene expression as a model system of retrograde or stress signaling between mitochondria and the nucleus, MYB DOMAIN PROTEIN29 (MYB29) was identified as a negative regulator (regulator of alternative oxidase1a 7 [rao7] mutant) in a genetic screen of Arabidopsis (Arabidopsis thaliana). rao7/ myb29 mutants have increased levels of AOX1a transcript and protein compared to wild type after induction with antimycin A. A variety of genes previously associated with the mitochondrial stress response also display enhanced transcript abundance, indicating that RAO7/MYB29 negatively regulates mitochondrial stress responses in general. Meta-analysis of hormoneresponsive marker genes and identification of downstream transcription factor networks revealed that MYB29 functions in the complex interplay of ethylene, jasmonic acid, salicylic acid, and reactive oxygen species signaling by regulating the expression of various ETHYLENE RESPONSE FACTOR and WRKY transcription factors. Despite an enhanced induction of mitochondrial stress response genes, rao7/myb29 mutants displayed an increased sensitivity to combined moderate light and drought stress. These results uncover interactions between mitochondrial retrograde signaling and the regulation of glucosinolate biosynthesis, both regulated by RAO7/MYB29. This common regulator can explain why perturbation of the mitochondrial function leads to transcriptomic responses overlapping with responses to biotic stress.

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published
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Plant Physiology
volume
173
issue
3
pages
19 pages
publisher
American Society of Plant Biologists
external identifiers
  • scopus:85014704748
ISSN
0032-0889
DOI
10.1104/pp.16.01494
language
English
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9d0ee5ab-4c13-48a4-80ee-119011f0561a
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2017-05-08 09:40:11
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2018-01-07 12:02:06
@article{9d0ee5ab-4c13-48a4-80ee-119011f0561a,
  abstract     = {<p>Plants sense and integrate a variety of signals from the environment through different interacting signal transduction pathways that involve hormones and signaling molecules. Using ALTERNATIVE OXIDASE1a (AOX1a) gene expression as a model system of retrograde or stress signaling between mitochondria and the nucleus, MYB DOMAIN PROTEIN29 (MYB29) was identified as a negative regulator (regulator of alternative oxidase1a 7 [rao7] mutant) in a genetic screen of Arabidopsis (Arabidopsis thaliana). rao7/ myb29 mutants have increased levels of AOX1a transcript and protein compared to wild type after induction with antimycin A. A variety of genes previously associated with the mitochondrial stress response also display enhanced transcript abundance, indicating that RAO7/MYB29 negatively regulates mitochondrial stress responses in general. Meta-analysis of hormoneresponsive marker genes and identification of downstream transcription factor networks revealed that MYB29 functions in the complex interplay of ethylene, jasmonic acid, salicylic acid, and reactive oxygen species signaling by regulating the expression of various ETHYLENE RESPONSE FACTOR and WRKY transcription factors. Despite an enhanced induction of mitochondrial stress response genes, rao7/myb29 mutants displayed an increased sensitivity to combined moderate light and drought stress. These results uncover interactions between mitochondrial retrograde signaling and the regulation of glucosinolate biosynthesis, both regulated by RAO7/MYB29. This common regulator can explain why perturbation of the mitochondrial function leads to transcriptomic responses overlapping with responses to biotic stress.</p>},
  author       = {Zhang, Xinhua and Ivanova, Aneta and Vandepoele, Klaas and Radomiljac, Jordan and Van De Velde, Jan and Berkowitz, Oliver and Willems, Patrick and Xu, Yue and Ng, Sophia and Van Aken, Olivier and Duncan, Owen and Zhang, Botao and Storme, Veronique and Chan, Kai Xun and Vaneechoutte, Dries and Pogson, Barry James and Van Breusegem, Frank and Whelan, James and De Clercq, Inge},
  issn         = {0032-0889},
  language     = {eng},
  month        = {03},
  number       = {3},
  pages        = {1825--1843},
  publisher    = {American Society of Plant Biologists},
  series       = {Plant Physiology},
  title        = {The transcription factor MYB29 is a regulator of alternative oxidase1a},
  url          = {http://dx.doi.org/10.1104/pp.16.01494},
  volume       = {173},
  year         = {2017},
}