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Islet β-cytotoxic monoclonal antibody against glycolipids in experimental diabetes induced by low dose streptozotocin and Freund's adjuvant

Ziegler, M. ; Teneberg, S. ; Witt, S. ; Ziegler, B. ; Hehmke, B. ; Kohnert, K. D. ; Egeberg, J. ; Karlsson, K. A. and Lernmark, A. LU orcid (1988) In Journal of Immunology 140(12). p.4144-4150
Abstract

Diabetes was induced in BALB/c mice by four injections of a subdiabetogenic dose (40 mg/kg) of streptozotocin in combination with CFA. The treatment increased the plasma glucose from 5.8 ± 0.1 to 22.1 ± 1.3 mmol/liter (n = 9). The diabetic animals had circulating islet cell surface antibodies (75%), and a monoclonal islet cell surface IgM antibody, K56aF3, generated from one of the diabetic BALB/c mice, mediated C-Dependent cytotoxicity against insulin-producing cells and inhibited glucose-stimulated insulin release from isolated rat islets. Solid phase assay on thin layer chromatograms showed no binding of the K56aF3 antibody to glycolipids prepared from relevant cells. However, testing against a series of glycolipids of various... (More)

Diabetes was induced in BALB/c mice by four injections of a subdiabetogenic dose (40 mg/kg) of streptozotocin in combination with CFA. The treatment increased the plasma glucose from 5.8 ± 0.1 to 22.1 ± 1.3 mmol/liter (n = 9). The diabetic animals had circulating islet cell surface antibodies (75%), and a monoclonal islet cell surface IgM antibody, K56aF3, generated from one of the diabetic BALB/c mice, mediated C-Dependent cytotoxicity against insulin-producing cells and inhibited glucose-stimulated insulin release from isolated rat islets. Solid phase assay on thin layer chromatograms showed no binding of the K56aF3 antibody to glycolipids prepared from relevant cells. However, testing against a series of glycolipids of various non-pancreatic origins showed a preferential binding to a nine-sugar glycolipid isolated from human erythrocytes carrying an unusual blood group A determinant (type 3). It is suggested that this mAb may be associated with the development of diabetes following a combination of polyclonal activation and non-diabetogenic doses of streptozotocin.

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author
; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Journal of Immunology
volume
140
issue
12
pages
7 pages
publisher
American Association of Immunologists
external identifiers
  • pmid:2967328
  • scopus:0023705399
ISSN
0022-1767
language
English
LU publication?
no
id
9d3a497a-e98b-4332-8b55-eb3c1d427960
date added to LUP
2019-09-11 10:06:03
date last changed
2026-01-10 07:03:13
@article{9d3a497a-e98b-4332-8b55-eb3c1d427960,
  abstract     = {{<p>Diabetes was induced in BALB/c mice by four injections of a subdiabetogenic dose (40 mg/kg) of streptozotocin in combination with CFA. The treatment increased the plasma glucose from 5.8 ± 0.1 to 22.1 ± 1.3 mmol/liter (n = 9). The diabetic animals had circulating islet cell surface antibodies (75%), and a monoclonal islet cell surface IgM antibody, K56aF3, generated from one of the diabetic BALB/c mice, mediated C-Dependent cytotoxicity against insulin-producing cells and inhibited glucose-stimulated insulin release from isolated rat islets. Solid phase assay on thin layer chromatograms showed no binding of the K56aF3 antibody to glycolipids prepared from relevant cells. However, testing against a series of glycolipids of various non-pancreatic origins showed a preferential binding to a nine-sugar glycolipid isolated from human erythrocytes carrying an unusual blood group A determinant (type 3). It is suggested that this mAb may be associated with the development of diabetes following a combination of polyclonal activation and non-diabetogenic doses of streptozotocin.</p>}},
  author       = {{Ziegler, M. and Teneberg, S. and Witt, S. and Ziegler, B. and Hehmke, B. and Kohnert, K. D. and Egeberg, J. and Karlsson, K. A. and Lernmark, A.}},
  issn         = {{0022-1767}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{12}},
  pages        = {{4144--4150}},
  publisher    = {{American Association of Immunologists}},
  series       = {{Journal of Immunology}},
  title        = {{Islet β-cytotoxic monoclonal antibody against glycolipids in experimental diabetes induced by low dose streptozotocin and Freund's adjuvant}},
  volume       = {{140}},
  year         = {{1988}},
}