Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Decorating Nanostructured Surfaces with Antimicrobial Peptides to Efficiently Fight Bacteria

Rigo, Serena ; Hürlimann, Dimitri ; Marot, Laurent ; Malmsten, Martin LU ; Meier, Wolfgang and Palivan, Cornelia G. (2020) In Bioresources and Bioprocessing 3(3). p.1533-1543
Abstract

With conventional antibiotic therapies being increasingly ineffective, bacterial infections with subsequent biofilm formation represent a global threat to human health. Here, an active and a passive strategy based on polymeric micelles were combined to fight bacterial growth. The passive strategy involved covalent immobilization of polymeric micelles through Michael addition between exposed maleimide and thiol functionalized surfaces. Compared to the bare surface, micelle-decorated surfaces showed reduced adherence and survival of bacteria. To extend this passive defense against bacteria with an active strategy, the immobilized micelles were equipped with the antimicrobial peptide KYE28 (KYEITTIHNLFRKLTHRLFRRNFGYTLR). The peptide... (More)

With conventional antibiotic therapies being increasingly ineffective, bacterial infections with subsequent biofilm formation represent a global threat to human health. Here, an active and a passive strategy based on polymeric micelles were combined to fight bacterial growth. The passive strategy involved covalent immobilization of polymeric micelles through Michael addition between exposed maleimide and thiol functionalized surfaces. Compared to the bare surface, micelle-decorated surfaces showed reduced adherence and survival of bacteria. To extend this passive defense against bacteria with an active strategy, the immobilized micelles were equipped with the antimicrobial peptide KYE28 (KYEITTIHNLFRKLTHRLFRRNFGYTLR). The peptide interacted nonspecifically with the immobilized micelles where it retained its antimicrobial property. The successful surface decoration with KYE28 was demonstrated by a combination of X-ray photoelectron spectroscopy and quartz crystal microbalance with dissipation monitoring. The initial antimicrobial activity of the nanostructured surfaces against Escherichia coli was found to be increased by the presence of KYE28. The combination of the active and passive strategy represents a straightforward modular approach that can easily be adapted, for example, by exchanging the antimicrobial peptide to optimize potency against challenging bacterial strains, and/or to simultaneously achieve antimicrobial and anti-infection properties.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
active, antimicrobial peptide, bacteria, decoration, passive, polymeric micelle
in
Bioresources and Bioprocessing
volume
3
issue
3
pages
11 pages
publisher
Springer
external identifiers
  • scopus:85080067101
ISSN
2197-4365
DOI
10.1021/acsabm.9b01154
language
English
LU publication?
yes
id
9d3c56ee-d250-4771-be5a-80f3191da2c9
date added to LUP
2020-12-28 11:34:22
date last changed
2022-04-26 22:47:32
@article{9d3c56ee-d250-4771-be5a-80f3191da2c9,
  abstract     = {{<p>With conventional antibiotic therapies being increasingly ineffective, bacterial infections with subsequent biofilm formation represent a global threat to human health. Here, an active and a passive strategy based on polymeric micelles were combined to fight bacterial growth. The passive strategy involved covalent immobilization of polymeric micelles through Michael addition between exposed maleimide and thiol functionalized surfaces. Compared to the bare surface, micelle-decorated surfaces showed reduced adherence and survival of bacteria. To extend this passive defense against bacteria with an active strategy, the immobilized micelles were equipped with the antimicrobial peptide KYE28 (KYEITTIHNLFRKLTHRLFRRNFGYTLR). The peptide interacted nonspecifically with the immobilized micelles where it retained its antimicrobial property. The successful surface decoration with KYE28 was demonstrated by a combination of X-ray photoelectron spectroscopy and quartz crystal microbalance with dissipation monitoring. The initial antimicrobial activity of the nanostructured surfaces against Escherichia coli was found to be increased by the presence of KYE28. The combination of the active and passive strategy represents a straightforward modular approach that can easily be adapted, for example, by exchanging the antimicrobial peptide to optimize potency against challenging bacterial strains, and/or to simultaneously achieve antimicrobial and anti-infection properties.</p>}},
  author       = {{Rigo, Serena and Hürlimann, Dimitri and Marot, Laurent and Malmsten, Martin and Meier, Wolfgang and Palivan, Cornelia G.}},
  issn         = {{2197-4365}},
  keywords     = {{active; antimicrobial peptide; bacteria; decoration; passive; polymeric micelle}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{1533--1543}},
  publisher    = {{Springer}},
  series       = {{Bioresources and Bioprocessing}},
  title        = {{Decorating Nanostructured Surfaces with Antimicrobial Peptides to Efficiently Fight Bacteria}},
  url          = {{http://dx.doi.org/10.1021/acsabm.9b01154}},
  doi          = {{10.1021/acsabm.9b01154}},
  volume       = {{3}},
  year         = {{2020}},
}