Expression pattern analysis of transcribed HERV sequences is complicated by ex vivo recombination
(2007) In Retrovirology 4.- Abstract
- Background: The human genome comprises numerous human endogenous retroviruses ( HERVs) that formed millions of years ago in ancestral species. A number of loci of the HERV-K(HML-2) family are evolutionarily much younger. A recent study suggested an infectious HERV-K(HML-2) variant in humans and other primates. Isolating such a varian t from human individuals would be a significant finding for human biology. Results: When investigating expression patterns of specific HML-2 proviruses we encountered HERV-K(HML-2) cDNA sequences without proviral homologues in the human genome, named HERV-KX, that could very well support recently suggested infectious HML-2 variants. However, detailed sequence analysis, using the software RECCO, suggested that... (More)
- Background: The human genome comprises numerous human endogenous retroviruses ( HERVs) that formed millions of years ago in ancestral species. A number of loci of the HERV-K(HML-2) family are evolutionarily much younger. A recent study suggested an infectious HERV-K(HML-2) variant in humans and other primates. Isolating such a varian t from human individuals would be a significant finding for human biology. Results: When investigating expression patterns of specific HML-2 proviruses we encountered HERV-K(HML-2) cDNA sequences without proviral homologues in the human genome, named HERV-KX, that could very well support recently suggested infectious HML-2 variants. However, detailed sequence analysis, using the software RECCO, suggested that HERV-KX sequences were produced by recombination, possibly arising ex vivo, between transcripts from different HML-2 proviral loci. Conclusion: As RT-PCR probably will be instrumental for isolating an infectious HERV-K(HML2) variant, generation of "new" HERV-K(HML-2) sequences by ex vivo recombination seems inevitable. Further complicated by an unknown amount of allelic sequence variation in HERV-K(HML-2) proviruses, newly identified HERV-K(HML-2) variants should be interpreted very cautiously. (Less)
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- author
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Retrovirology
- volume
- 4
- publisher
- BioMed Central (BMC)
- external identifiers
-
- wos:000247633300001
- scopus:34447301254
- ISSN
- 1742-4690
- DOI
- 10.1186/1742-4690-4-39
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Molecular Virology (013212007)
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- 9d401ba5-7ed1-4301-aa49-84fbe17e0c04 (old id 646162)
- date added to LUP
- 2016-04-01 16:48:40
- date last changed
- 2022-03-22 21:19:18
@article{9d401ba5-7ed1-4301-aa49-84fbe17e0c04, abstract = {{Background: The human genome comprises numerous human endogenous retroviruses ( HERVs) that formed millions of years ago in ancestral species. A number of loci of the HERV-K(HML-2) family are evolutionarily much younger. A recent study suggested an infectious HERV-K(HML-2) variant in humans and other primates. Isolating such a varian t from human individuals would be a significant finding for human biology. Results: When investigating expression patterns of specific HML-2 proviruses we encountered HERV-K(HML-2) cDNA sequences without proviral homologues in the human genome, named HERV-KX, that could very well support recently suggested infectious HML-2 variants. However, detailed sequence analysis, using the software RECCO, suggested that HERV-KX sequences were produced by recombination, possibly arising ex vivo, between transcripts from different HML-2 proviral loci. Conclusion: As RT-PCR probably will be instrumental for isolating an infectious HERV-K(HML2) variant, generation of "new" HERV-K(HML-2) sequences by ex vivo recombination seems inevitable. Further complicated by an unknown amount of allelic sequence variation in HERV-K(HML-2) proviruses, newly identified HERV-K(HML-2) variants should be interpreted very cautiously.}}, author = {{Flockerzi, Aline and Maydt, Jochen and Frank, Oliver and Ruggieri, Alessia and Maldener, Esther and Seifarth, Wolfgang and Medstrand, Patrik and Lengauer, Thomas and Meyerhans, Andreas and Leib-Moesch, Christine and Meese, Eckart and Mayer, Jens}}, issn = {{1742-4690}}, language = {{eng}}, publisher = {{BioMed Central (BMC)}}, series = {{Retrovirology}}, title = {{Expression pattern analysis of transcribed HERV sequences is complicated by ex vivo recombination}}, url = {{http://dx.doi.org/10.1186/1742-4690-4-39}}, doi = {{10.1186/1742-4690-4-39}}, volume = {{4}}, year = {{2007}}, }