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A well-plate format isothermal multi-channel microcalorimeter for monitoring the activity of living cells and tissues

Wadsö, Ingemar LU ; Hallén, Dan; Jansson, magnus; Suurkuusk, Jaak; Wenzler, Tanja and Braissant, Olivier (2017) In Thermochimica Acta 652. p.141-149
Abstract

Design and properties are reported for a novel type of multi-channel isothermal microcalorimeter. It is equipped with 48 calorimetric units (channels) and is primarily intended for use as a monitor of the activity of living cells, tissues and small animals. Calorimetric vessels are positioned in a holder with the format of a 48-well microtiter plate. At most, 47 samples can be measured simultaneously; one vessel is then used as reference. The standard configuration is 32 sample positions using 16 channels as references in a twin calorimeter setup. The detection limit is then 0.1 μW. Sample volumes are usually 100 μl–300 μl. The 24 h baseline stability is typically 0.2 μW (room temperature variation ≤1 °C). The instrument was designed... (More)

Design and properties are reported for a novel type of multi-channel isothermal microcalorimeter. It is equipped with 48 calorimetric units (channels) and is primarily intended for use as a monitor of the activity of living cells, tissues and small animals. Calorimetric vessels are positioned in a holder with the format of a 48-well microtiter plate. At most, 47 samples can be measured simultaneously; one vessel is then used as reference. The standard configuration is 32 sample positions using 16 channels as references in a twin calorimeter setup. The detection limit is then 0.1 μW. Sample volumes are usually 100 μl–300 μl. The 24 h baseline stability is typically 0.2 μW (room temperature variation ≤1 °C). The instrument was designed considering feasible uses in applied biology, especially in pharmaceutical and clinical laboratories and in environmental work. However, it can be employed as a general monitor of slow processes in different fields of biology and for non-biological systems, including accurate determination of their thermal powers (heat production rates). In the present report, properties of the instrument are characterized by chemical calibration experiments and in measurements of growth of bacteria and mammalian cells.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bacteria, Isothermal microcalorimeter, Mammalian cells, Multi-channel
in
Thermochimica Acta
volume
652
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:85018509280
  • wos:000403861000017
ISSN
0040-6031
DOI
10.1016/j.tca.2017.03.010
language
English
LU publication?
yes
id
9dd6760e-a42d-4a95-9e91-0ec255775eb5
date added to LUP
2017-05-17 10:01:22
date last changed
2017-09-18 11:36:00
@article{9dd6760e-a42d-4a95-9e91-0ec255775eb5,
  abstract     = {<p>Design and properties are reported for a novel type of multi-channel isothermal microcalorimeter. It is equipped with 48 calorimetric units (channels) and is primarily intended for use as a monitor of the activity of living cells, tissues and small animals. Calorimetric vessels are positioned in a holder with the format of a 48-well microtiter plate. At most, 47 samples can be measured simultaneously; one vessel is then used as reference. The standard configuration is 32 sample positions using 16 channels as references in a twin calorimeter setup. The detection limit is then 0.1 μW. Sample volumes are usually 100 μl–300 μl. The 24 h baseline stability is typically 0.2 μW (room temperature variation ≤1 °C). The instrument was designed considering feasible uses in applied biology, especially in pharmaceutical and clinical laboratories and in environmental work. However, it can be employed as a general monitor of slow processes in different fields of biology and for non-biological systems, including accurate determination of their thermal powers (heat production rates). In the present report, properties of the instrument are characterized by chemical calibration experiments and in measurements of growth of bacteria and mammalian cells.</p>},
  author       = {Wadsö, Ingemar and Hallén, Dan and Jansson, magnus and Suurkuusk, Jaak and Wenzler, Tanja and Braissant, Olivier},
  issn         = {0040-6031},
  keyword      = {Bacteria,Isothermal microcalorimeter,Mammalian cells,Multi-channel},
  language     = {eng},
  month        = {06},
  pages        = {141--149},
  publisher    = {Elsevier},
  series       = {Thermochimica Acta},
  title        = {A well-plate format isothermal multi-channel microcalorimeter for monitoring the activity of living cells and tissues},
  url          = {http://dx.doi.org/10.1016/j.tca.2017.03.010},
  volume       = {652},
  year         = {2017},
}