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History and results of the two inter-laboratory round robin endotoxin assay studies on cotton dust

Chun, DTW; Bartlett, K; Gordon, T; Jacobs, RR; Larsson, BM; Larsson, Lennart LU ; Lewis, DM; Liesivuori, J; Michel, O and Milton, DK, et al. (2006) 5th International Symposium on Future of Rural Peoples - Rural Economy, Healthy People, Environment, Rural Communities 49(4). p.301-306
Abstract
Background In the US cotton industry, airborne cotton dust levels are regulated, and other countries are moving to specify safety limits for airborne endotoxins. There is concern about potential respiratory health hazards associated with agricultural and otherorganic dusts. In laboratories, ranking which samples have high and low levels of endotoxin is usually in good agreement between laboratories. When different laboratories assay identical samples, the levels differ The objective of this research was to evaluate the intra- and inter-laboratory variability for 13 laboratories measuring endotoxin in cotton dust. Method Two inter-laboratory round robin endotoxin assay studies were conducted using cotton dust. In the first round robin, each... (More)
Background In the US cotton industry, airborne cotton dust levels are regulated, and other countries are moving to specify safety limits for airborne endotoxins. There is concern about potential respiratory health hazards associated with agricultural and otherorganic dusts. In laboratories, ranking which samples have high and low levels of endotoxin is usually in good agreement between laboratories. When different laboratories assay identical samples, the levels differ The objective of this research was to evaluate the intra- and inter-laboratory variability for 13 laboratories measuring endotoxin in cotton dust. Method Two inter-laboratory round robin endotoxin assay studies were conducted using cotton dust. In the first round robin, each laboratory used their normal in-house assay method and then used a common extraction protocol. In the second round robin, a common extraction protocol and endotoxin assay, kit was used. Results The inter-laboratory, results using a common extraction protocol showed reduced differences. Using the same extraction protocol and endotoxin assay kit, the intra-laboratory variation was small and inter-laboratory variation was reduced but not enough for inter-laboratory agreement. Most of the laboratories were able to discern between the high and low endotoxin concentration dusts. Conclusions Standardization has reduced the differences in results between laboratories and possibly further standardization may bring closer inter-laboratory agreement. (Less)
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organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
endotoxin assay, limulus amoebocyte lysate test (LAL), study, cotton dust, round robin endotoxin assay, inter-laboratory endotoxin assay study, intra-laboratory endotoxin assay study, lipopolysaccharides (LPS)
host publication
American Journal of Industrial Medicine
volume
49
issue
4
pages
301 - 306
publisher
John Wiley & Sons
conference name
5th International Symposium on Future of Rural Peoples - Rural Economy, Healthy People, Environment, Rural Communities
conference location
Saskatoon, Canada
conference dates
2004-10-19
external identifiers
  • wos:000236824900010
  • pmid:16526062
  • scopus:33646074281
ISSN
1097-0274
0271-3586
DOI
10.1002/ajim.20266
language
English
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yes
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9de9e951-350f-4bd8-8238-77cff1abfda4 (old id 414245)
date added to LUP
2007-10-01 18:52:21
date last changed
2019-03-12 01:35:19
@inproceedings{9de9e951-350f-4bd8-8238-77cff1abfda4,
  abstract     = {Background In the US cotton industry, airborne cotton dust levels are regulated, and other countries are moving to specify safety limits for airborne endotoxins. There is concern about potential respiratory health hazards associated with agricultural and otherorganic dusts. In laboratories, ranking which samples have high and low levels of endotoxin is usually in good agreement between laboratories. When different laboratories assay identical samples, the levels differ The objective of this research was to evaluate the intra- and inter-laboratory variability for 13 laboratories measuring endotoxin in cotton dust. Method Two inter-laboratory round robin endotoxin assay studies were conducted using cotton dust. In the first round robin, each laboratory used their normal in-house assay method and then used a common extraction protocol. In the second round robin, a common extraction protocol and endotoxin assay, kit was used. Results The inter-laboratory, results using a common extraction protocol showed reduced differences. Using the same extraction protocol and endotoxin assay kit, the intra-laboratory variation was small and inter-laboratory variation was reduced but not enough for inter-laboratory agreement. Most of the laboratories were able to discern between the high and low endotoxin concentration dusts. Conclusions Standardization has reduced the differences in results between laboratories and possibly further standardization may bring closer inter-laboratory agreement.},
  author       = {Chun, DTW and Bartlett, K and Gordon, T and Jacobs, RR and Larsson, BM and Larsson, Lennart and Lewis, DM and Liesivuori, J and Michel, O and Milton, DK and Rylander, R and Thorne, PS and White, EM and Brown, ME and Gunn, VS and Wurtz, H},
  issn         = {1097-0274},
  keyword      = {endotoxin assay,limulus amoebocyte lysate test (LAL),study,cotton dust,round robin endotoxin assay,inter-laboratory endotoxin assay study,intra-laboratory endotoxin assay study,lipopolysaccharides (LPS)},
  language     = {eng},
  location     = {Saskatoon, Canada},
  number       = {4},
  pages        = {301--306},
  publisher    = {John Wiley & Sons},
  title        = {History and results of the two inter-laboratory round robin endotoxin assay studies on cotton dust},
  url          = {http://dx.doi.org/10.1002/ajim.20266},
  volume       = {49},
  year         = {2006},
}