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Validated Reference Panel from Renewable Source of Genomic DNA Available for Standardization of Blood Group Genotyping

Volkova, Evgeniya; Sippert, Emilia; Liu, Meihong; Mercado, Teresita; Denomme, Gregory A; Illoh, Orieji; Liu, Zhugong; Rios, Maria and , (2019) In Journal of Molecular Diagnostics 21(3). p.525-537
Abstract

Extended blood group genotyping is an invaluable tool used for prevention of alloimmunization. Genotyping is particularly suitable when antigens are weak, specific antisera are unavailable, or accurate phenotyping is problematic because of a disease state or recent transfusions. In addition, genotyping facilitates establishment of mass-scale patient-matched donor databases. However, standardization of genotyping technologies has been hindered by the lack of reference panels. A well-characterized renewable reference panel for standardization of blood group genotyping was developed. The panel consists of genomic DNA lyophilized and stored in glass vials. Genomic DNA was extracted in bulk from immortalized lymphoblastoid cell lines,... (More)

Extended blood group genotyping is an invaluable tool used for prevention of alloimmunization. Genotyping is particularly suitable when antigens are weak, specific antisera are unavailable, or accurate phenotyping is problematic because of a disease state or recent transfusions. In addition, genotyping facilitates establishment of mass-scale patient-matched donor databases. However, standardization of genotyping technologies has been hindered by the lack of reference panels. A well-characterized renewable reference panel for standardization of blood group genotyping was developed. The panel consists of genomic DNA lyophilized and stored in glass vials. Genomic DNA was extracted in bulk from immortalized lymphoblastoid cell lines, generated by Epstein-Barr virus transformation of peripheral blood lymphocytes harvested from volunteer blood donors. The panel was validated by an international collaborative study involving 28 laboratories that tested each DNA panel member for 41 polymorphisms associated with 17 blood group systems. Overall, analysis of genotyping results showed >98% agreement with the expected outcomes, demonstrating suitability of the material for use as reference. Highest levels of discordance were observed for the genes CR1, CD55, BSG, and RHD. Although limited, observed inconsistencies and procedural limitations reinforce the importance of reference reagents to standardize and harmonize results. Results of stability and accelerated degradation studies support the suitability of this panel for use as reference reagent for blood group genotyping assay development and standardization.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Molecular Diagnostics
volume
21
issue
3
pages
525 - 537
publisher
Elsevier Inc.
external identifiers
  • scopus:85064549608
ISSN
1525-1578
DOI
10.1016/j.jmoldx.2019.02.003
language
English
LU publication?
yes
id
9dec8567-7756-4183-9127-9fa3dfdb686f
date added to LUP
2019-05-20 13:29:00
date last changed
2019-08-14 02:18:45
@article{9dec8567-7756-4183-9127-9fa3dfdb686f,
  abstract     = {<p>Extended blood group genotyping is an invaluable tool used for prevention of alloimmunization. Genotyping is particularly suitable when antigens are weak, specific antisera are unavailable, or accurate phenotyping is problematic because of a disease state or recent transfusions. In addition, genotyping facilitates establishment of mass-scale patient-matched donor databases. However, standardization of genotyping technologies has been hindered by the lack of reference panels. A well-characterized renewable reference panel for standardization of blood group genotyping was developed. The panel consists of genomic DNA lyophilized and stored in glass vials. Genomic DNA was extracted in bulk from immortalized lymphoblastoid cell lines, generated by Epstein-Barr virus transformation of peripheral blood lymphocytes harvested from volunteer blood donors. The panel was validated by an international collaborative study involving 28 laboratories that tested each DNA panel member for 41 polymorphisms associated with 17 blood group systems. Overall, analysis of genotyping results showed &gt;98% agreement with the expected outcomes, demonstrating suitability of the material for use as reference. Highest levels of discordance were observed for the genes CR1, CD55, BSG, and RHD. Although limited, observed inconsistencies and procedural limitations reinforce the importance of reference reagents to standardize and harmonize results. Results of stability and accelerated degradation studies support the suitability of this panel for use as reference reagent for blood group genotyping assay development and standardization.</p>},
  author       = {Volkova, Evgeniya and Sippert, Emilia and Liu, Meihong and Mercado, Teresita and Denomme, Gregory A and Illoh, Orieji and Liu, Zhugong and Rios, Maria and , },
  issn         = {1525-1578},
  language     = {eng},
  number       = {3},
  pages        = {525--537},
  publisher    = {Elsevier Inc.},
  series       = {Journal of Molecular Diagnostics},
  title        = {Validated Reference Panel from Renewable Source of Genomic DNA Available for Standardization of Blood Group Genotyping},
  url          = {http://dx.doi.org/10.1016/j.jmoldx.2019.02.003},
  volume       = {21},
  year         = {2019},
}