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Nursing and training of pigs used in renal transplantation studies

Rydén, Anneli ; Manell, Elin ; Biglarnia, Alireza LU ; Hedenqvist, Patricia ; Strandberg, Gabriel LU ; Ley, Charles ; Hansson, Kerstin ; Nyman, Görel and Jensen-Waern, Marianne (2019) In Laboratory Animals
Abstract

The pig is commonly used in renal transplantation studies since the porcine kidney resembles the human kidney. To meet the requirements of intense caretaking and examination without stress, a 2-week socialisation and training programme was developed. Conventional cross-breed pigs (n = 36) with high health status were trained for 15 min/day in a four-step training programme before kidney transplantation. The systematic training resulted in calm animals, which allowed for ultrasound examination, blood sampling and urine sampling without restraint. When a 2-methacryloyloxyethyl phosphorylcholine polymer-coated jugular catheter introduced via the auricular vein was used for post-operative blood sampling, clotting was avoided. To assess... (More)

The pig is commonly used in renal transplantation studies since the porcine kidney resembles the human kidney. To meet the requirements of intense caretaking and examination without stress, a 2-week socialisation and training programme was developed. Conventional cross-breed pigs (n = 36) with high health status were trained for 15 min/day in a four-step training programme before kidney transplantation. The systematic training resulted in calm animals, which allowed for ultrasound examination, blood sampling and urine sampling without restraint. When a 2-methacryloyloxyethyl phosphorylcholine polymer-coated jugular catheter introduced via the auricular vein was used for post-operative blood sampling, clotting was avoided. To assess renal function, urinary output was observed and creatinine and cystatin C were measured; the latter was not found to be useful in recently transplanted pigs. The results presented contribute to the 3Rs (refine, reduce, replace).

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author
; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
indwelling catheter, kidney transplantation, swine, urinary bladder
in
Laboratory Animals
publisher
Royal Society of Medicine Press
external identifiers
  • pmid:31648591
  • scopus:85074613428
ISSN
0023-6772
DOI
10.1177/0023677219879169
language
English
LU publication?
no
id
9df461ef-c23f-4c8b-aa64-150357169506
date added to LUP
2019-11-21 13:48:29
date last changed
2020-01-13 02:32:25
@article{9df461ef-c23f-4c8b-aa64-150357169506,
  abstract     = {<p>The pig is commonly used in renal transplantation studies since the porcine kidney resembles the human kidney. To meet the requirements of intense caretaking and examination without stress, a 2-week socialisation and training programme was developed. Conventional cross-breed pigs (n = 36) with high health status were trained for 15 min/day in a four-step training programme before kidney transplantation. The systematic training resulted in calm animals, which allowed for ultrasound examination, blood sampling and urine sampling without restraint. When a 2-methacryloyloxyethyl phosphorylcholine polymer-coated jugular catheter introduced via the auricular vein was used for post-operative blood sampling, clotting was avoided. To assess renal function, urinary output was observed and creatinine and cystatin C were measured; the latter was not found to be useful in recently transplanted pigs. The results presented contribute to the 3Rs (refine, reduce, replace).</p>},
  author       = {Rydén, Anneli and Manell, Elin and Biglarnia, Alireza and Hedenqvist, Patricia and Strandberg, Gabriel and Ley, Charles and Hansson, Kerstin and Nyman, Görel and Jensen-Waern, Marianne},
  issn         = {0023-6772},
  language     = {eng},
  month        = {10},
  publisher    = {Royal Society of Medicine Press},
  series       = {Laboratory Animals},
  title        = {Nursing and training of pigs used in renal transplantation studies},
  url          = {http://dx.doi.org/10.1177/0023677219879169},
  doi          = {10.1177/0023677219879169},
  year         = {2019},
}