Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Quantification of platelet activation status by analyzing P-selectin expression

Leytin, Valery ; Mody, Meera ; Semple, John W. LU ; Garvey, Bernadette and Freedman, John (2000) In Biochemical and Biophysical Research Communications 273(2). p.565-570
Abstract

Platelet activation status (PAS) is used for characterizing quality and function of platelets in various experimental and clinical settings. In this study, we created a set of platelet populations differing in PAS, using stimulation of platelets with thrombin in a wide range of concentrations, and analyzed a number of flow cytometric parameters, which characterize PAS by measuring P-selectin (CD62) expression. We found that PAS of a platelet population depends significantly on the specific parameters used for detecting CD62 expression and can differ several fold. We revealed the parameters which are more sensitive for distinguishing the differences between populations with similar low and similar high PAS. Selection of valid and... (More)

Platelet activation status (PAS) is used for characterizing quality and function of platelets in various experimental and clinical settings. In this study, we created a set of platelet populations differing in PAS, using stimulation of platelets with thrombin in a wide range of concentrations, and analyzed a number of flow cytometric parameters, which characterize PAS by measuring P-selectin (CD62) expression. We found that PAS of a platelet population depends significantly on the specific parameters used for detecting CD62 expression and can differ several fold. We revealed the parameters which are more sensitive for distinguishing the differences between populations with similar low and similar high PAS. Selection of valid and sensitive flow cytometric parameters for PAS evaluation and distinguishing the differences between platelet populations with similar PAS can serve for diagnosis of platelet-associated disorders and monitoring their course and therapeutic interventions. (C) 2000 Academic Press.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
CD62, Flow cytometry, P-selectin, Platelet activation status, Thrombin
in
Biochemical and Biophysical Research Communications
volume
273
issue
2
pages
6 pages
publisher
Elsevier
external identifiers
  • pmid:10873646
  • scopus:0033894566
ISSN
0006-291X
DOI
10.1006/bbrc.2000.2977
language
English
LU publication?
no
id
9df4a427-e348-4679-85a8-43261f85fcbb
date added to LUP
2019-12-03 10:28:27
date last changed
2024-04-17 00:42:44
@article{9df4a427-e348-4679-85a8-43261f85fcbb,
  abstract     = {{<p>Platelet activation status (PAS) is used for characterizing quality and function of platelets in various experimental and clinical settings. In this study, we created a set of platelet populations differing in PAS, using stimulation of platelets with thrombin in a wide range of concentrations, and analyzed a number of flow cytometric parameters, which characterize PAS by measuring P-selectin (CD62) expression. We found that PAS of a platelet population depends significantly on the specific parameters used for detecting CD62 expression and can differ several fold. We revealed the parameters which are more sensitive for distinguishing the differences between populations with similar low and similar high PAS. Selection of valid and sensitive flow cytometric parameters for PAS evaluation and distinguishing the differences between platelet populations with similar PAS can serve for diagnosis of platelet-associated disorders and monitoring their course and therapeutic interventions. (C) 2000 Academic Press.</p>}},
  author       = {{Leytin, Valery and Mody, Meera and Semple, John W. and Garvey, Bernadette and Freedman, John}},
  issn         = {{0006-291X}},
  keywords     = {{CD62; Flow cytometry; P-selectin; Platelet activation status; Thrombin}},
  language     = {{eng}},
  month        = {{07}},
  number       = {{2}},
  pages        = {{565--570}},
  publisher    = {{Elsevier}},
  series       = {{Biochemical and Biophysical Research Communications}},
  title        = {{Quantification of platelet activation status by analyzing P-selectin expression}},
  url          = {{http://dx.doi.org/10.1006/bbrc.2000.2977}},
  doi          = {{10.1006/bbrc.2000.2977}},
  volume       = {{273}},
  year         = {{2000}},
}