Modulation of Protein-Interaction States through the Cell Cycle
Dai, Lingyun ; Zhao, Tianyun ; Bisteau, Xavier ; Sun, Wendi ; Prabhu, Nayana ; Lim, Yan Ting ; Sobota, Radoslaw M ; Kaldis, Philipp LU
and Nordlund, Pär
(2018)
In Cell
173(6).
p.1481-1494
- Abstract
Global profiling of protein expression through the cell cycle has revealed subsets of periodically expressed proteins. However, expression levels alone only give a partial view of the biochemical processes determining cellular events. Using a proteome-wide implementation of the cellular thermal shift assay (CETSA) to study specific cell-cycle phases, we uncover changes of interaction states for more than 750 proteins during the cell cycle. Notably, many protein complexes are modulated in specific cell-cycle phases, reflecting their roles in processes such as DNA replication, chromatin remodeling, transcription, translation, and disintegration of the nuclear envelope. Surprisingly, only small differences in the interaction states were... (More)
Global profiling of protein expression through the cell cycle has revealed subsets of periodically expressed proteins. However, expression levels alone only give a partial view of the biochemical processes determining cellular events. Using a proteome-wide implementation of the cellular thermal shift assay (CETSA) to study specific cell-cycle phases, we uncover changes of interaction states for more than 750 proteins during the cell cycle. Notably, many protein complexes are modulated in specific cell-cycle phases, reflecting their roles in processes such as DNA replication, chromatin remodeling, transcription, translation, and disintegration of the nuclear envelope. Surprisingly, only small differences in the interaction states were seen between the G1 and the G2 phase, suggesting similar hardwiring of biochemical processes in these two phases. The present work reveals novel molecular details of the cell cycle and establishes proteome-wide CETSA as a new strategy to study modulation of protein-interaction states in intact cells.
(Less)
- author
- Dai, Lingyun
; Zhao, Tianyun
; Bisteau, Xavier
; Sun, Wendi
; Prabhu, Nayana
; Lim, Yan Ting
; Sobota, Radoslaw M
; Kaldis, Philipp
LU
and Nordlund, Pär
- publishing date
- 2018-05-31
- type
- Contribution to journal
- publication status
- published
- keywords
- Cell Cycle, Cell Division, Chromatin/chemistry, Cluster Analysis, DNA Replication, G1 Phase, G2 Phase, Humans, K562 Cells, Nuclear Envelope, Protein Interaction Mapping, Proteome, Proteomics/methods
- in
- Cell
- volume
- 173
- issue
- 6
- pages
- 1481 - 1494
- publisher
- Cell Press
- external identifiers
-
- pmid:29706543
- scopus:85046143418
- ISSN
- 1097-4172
- DOI
- 10.1016/j.cell.2018.03.065
- language
- English
- LU publication?
- no
- id
- 9e21f467-14fb-43b9-93b4-1f06b43385c0
- date added to LUP
- 2019-09-17 14:44:20
- date last changed
- 2024-04-16 20:53:18
@article{9e21f467-14fb-43b9-93b4-1f06b43385c0,
abstract = {{<p>Global profiling of protein expression through the cell cycle has revealed subsets of periodically expressed proteins. However, expression levels alone only give a partial view of the biochemical processes determining cellular events. Using a proteome-wide implementation of the cellular thermal shift assay (CETSA) to study specific cell-cycle phases, we uncover changes of interaction states for more than 750 proteins during the cell cycle. Notably, many protein complexes are modulated in specific cell-cycle phases, reflecting their roles in processes such as DNA replication, chromatin remodeling, transcription, translation, and disintegration of the nuclear envelope. Surprisingly, only small differences in the interaction states were seen between the G1 and the G2 phase, suggesting similar hardwiring of biochemical processes in these two phases. The present work reveals novel molecular details of the cell cycle and establishes proteome-wide CETSA as a new strategy to study modulation of protein-interaction states in intact cells.</p>}},
author = {{Dai, Lingyun and Zhao, Tianyun and Bisteau, Xavier and Sun, Wendi and Prabhu, Nayana and Lim, Yan Ting and Sobota, Radoslaw M and Kaldis, Philipp and Nordlund, Pär}},
issn = {{1097-4172}},
keywords = {{Cell Cycle; Cell Division; Chromatin/chemistry; Cluster Analysis; DNA Replication; G1 Phase; G2 Phase; Humans; K562 Cells; Nuclear Envelope; Protein Interaction Mapping; Proteome; Proteomics/methods}},
language = {{eng}},
month = {{05}},
number = {{6}},
pages = {{1481--1494}},
publisher = {{Cell Press}},
series = {{Cell}},
title = {{Modulation of Protein-Interaction States through the Cell Cycle}},
url = {{http://dx.doi.org/10.1016/j.cell.2018.03.065}},
doi = {{10.1016/j.cell.2018.03.065}},
volume = {{173}},
year = {{2018}},
}