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Modulation of Protein-Interaction States through the Cell Cycle

Dai, Lingyun ; Zhao, Tianyun ; Bisteau, Xavier ; Sun, Wendi ; Prabhu, Nayana ; Lim, Yan Ting ; Sobota, Radoslaw M ; Kaldis, Philipp LU and Nordlund, Pär (2018) In Cell 173(6). p.1481-1494
Abstract

Global profiling of protein expression through the cell cycle has revealed subsets of periodically expressed proteins. However, expression levels alone only give a partial view of the biochemical processes determining cellular events. Using a proteome-wide implementation of the cellular thermal shift assay (CETSA) to study specific cell-cycle phases, we uncover changes of interaction states for more than 750 proteins during the cell cycle. Notably, many protein complexes are modulated in specific cell-cycle phases, reflecting their roles in processes such as DNA replication, chromatin remodeling, transcription, translation, and disintegration of the nuclear envelope. Surprisingly, only small differences in the interaction states were... (More)

Global profiling of protein expression through the cell cycle has revealed subsets of periodically expressed proteins. However, expression levels alone only give a partial view of the biochemical processes determining cellular events. Using a proteome-wide implementation of the cellular thermal shift assay (CETSA) to study specific cell-cycle phases, we uncover changes of interaction states for more than 750 proteins during the cell cycle. Notably, many protein complexes are modulated in specific cell-cycle phases, reflecting their roles in processes such as DNA replication, chromatin remodeling, transcription, translation, and disintegration of the nuclear envelope. Surprisingly, only small differences in the interaction states were seen between the G1 and the G2 phase, suggesting similar hardwiring of biochemical processes in these two phases. The present work reveals novel molecular details of the cell cycle and establishes proteome-wide CETSA as a new strategy to study modulation of protein-interaction states in intact cells.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Cell Cycle, Cell Division, Chromatin/chemistry, Cluster Analysis, DNA Replication, G1 Phase, G2 Phase, Humans, K562 Cells, Nuclear Envelope, Protein Interaction Mapping, Proteome, Proteomics/methods
in
Cell
volume
173
issue
6
pages
1481 - 1494
publisher
Cell Press
external identifiers
  • pmid:29706543
  • scopus:85046143418
ISSN
1097-4172
DOI
10.1016/j.cell.2018.03.065
language
English
LU publication?
no
id
9e21f467-14fb-43b9-93b4-1f06b43385c0
date added to LUP
2019-09-17 14:44:20
date last changed
2019-12-03 02:18:53
@article{9e21f467-14fb-43b9-93b4-1f06b43385c0,
  abstract     = {<p>Global profiling of protein expression through the cell cycle has revealed subsets of periodically expressed proteins. However, expression levels alone only give a partial view of the biochemical processes determining cellular events. Using a proteome-wide implementation of the cellular thermal shift assay (CETSA) to study specific cell-cycle phases, we uncover changes of interaction states for more than 750 proteins during the cell cycle. Notably, many protein complexes are modulated in specific cell-cycle phases, reflecting their roles in processes such as DNA replication, chromatin remodeling, transcription, translation, and disintegration of the nuclear envelope. Surprisingly, only small differences in the interaction states were seen between the G1 and the G2 phase, suggesting similar hardwiring of biochemical processes in these two phases. The present work reveals novel molecular details of the cell cycle and establishes proteome-wide CETSA as a new strategy to study modulation of protein-interaction states in intact cells.</p>},
  author       = {Dai, Lingyun and Zhao, Tianyun and Bisteau, Xavier and Sun, Wendi and Prabhu, Nayana and Lim, Yan Ting and Sobota, Radoslaw M and Kaldis, Philipp and Nordlund, Pär},
  issn         = {1097-4172},
  language     = {eng},
  month        = {05},
  number       = {6},
  pages        = {1481--1494},
  publisher    = {Cell Press},
  series       = {Cell},
  title        = {Modulation of Protein-Interaction States through the Cell Cycle},
  url          = {http://dx.doi.org/10.1016/j.cell.2018.03.065},
  doi          = {10.1016/j.cell.2018.03.065},
  volume       = {173},
  year         = {2018},
}