Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture
(2008) In Molecular and Cellular Neuroscience 38(2). p.58-245- Abstract
Stem cell lines that provide a renewable and scaleable supply of central nervous system cell types would constitute an invaluable resource for basic and applied neurobiology. Here we describe the generation and long-term expansion of multiple human foetal neural stem (NS) cell lines in monolayer culture without genetic immortalization. Adherent human NS cells are propagated in the presence of epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF2), under which conditions they stably express neural precursor markers and exhibit negligible differentiation into neurons or glia. However, they produce astrocytes, oligodendrocytes, and neurons upon exposure to appropriate differentiation factors. Single cell cloning demonstrates... (More)
Stem cell lines that provide a renewable and scaleable supply of central nervous system cell types would constitute an invaluable resource for basic and applied neurobiology. Here we describe the generation and long-term expansion of multiple human foetal neural stem (NS) cell lines in monolayer culture without genetic immortalization. Adherent human NS cells are propagated in the presence of epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF2), under which conditions they stably express neural precursor markers and exhibit negligible differentiation into neurons or glia. However, they produce astrocytes, oligodendrocytes, and neurons upon exposure to appropriate differentiation factors. Single cell cloning demonstrates that human NS cells are tripotent. They retain a diploid karyotype and constant neurogenic capacity after over 100 generations. In contrast to human neurospheres, we observe no requirement for the cytokine leukaemia inhibitory factor (LIF) for continued expansion of adherent human NS cells. Human NS cells can be stably transfected to provide reporter lines and readily imaged in live monolayer cultures, creating the potential for high content genetic and chemical screens.
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- author
- Sun, Yirui ; Pollard, Steven ; Conti, Luciano ; Toselli, Mauro ; Biella, Gerardo ; Parkin, Georgina ; Willatt, Lionel ; Falk, Anna LU ; Cattaneo, Elena and Smith, Austin
- publishing date
- 2008-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Astrocytes/cytology, Biomarkers/metabolism, Cell Culture Techniques/methods, Cell Differentiation/physiology, Cell Division/physiology, Cell Line, Clone Cells, Embryonic Stem Cells/cytology, Fetus/cytology, Green Fluorescent Proteins/genetics, Humans, Neural Cell Adhesion Molecule L1/metabolism, Neurons/cytology, Oligodendroglia/cytology, Sialic Acids/metabolism, Transfection
- in
- Molecular and Cellular Neuroscience
- volume
- 38
- issue
- 2
- pages
- 14 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:44649197736
- pmid:18450476
- ISSN
- 1044-7431
- DOI
- 10.1016/j.mcn.2008.02.014
- language
- English
- LU publication?
- no
- id
- 9e2662cf-d687-4504-b6c4-b2211b10fc62
- date added to LUP
- 2021-08-10 13:49:36
- date last changed
- 2024-04-06 05:34:24
@article{9e2662cf-d687-4504-b6c4-b2211b10fc62,
abstract = {{<p>Stem cell lines that provide a renewable and scaleable supply of central nervous system cell types would constitute an invaluable resource for basic and applied neurobiology. Here we describe the generation and long-term expansion of multiple human foetal neural stem (NS) cell lines in monolayer culture without genetic immortalization. Adherent human NS cells are propagated in the presence of epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF2), under which conditions they stably express neural precursor markers and exhibit negligible differentiation into neurons or glia. However, they produce astrocytes, oligodendrocytes, and neurons upon exposure to appropriate differentiation factors. Single cell cloning demonstrates that human NS cells are tripotent. They retain a diploid karyotype and constant neurogenic capacity after over 100 generations. In contrast to human neurospheres, we observe no requirement for the cytokine leukaemia inhibitory factor (LIF) for continued expansion of adherent human NS cells. Human NS cells can be stably transfected to provide reporter lines and readily imaged in live monolayer cultures, creating the potential for high content genetic and chemical screens.</p>}},
author = {{Sun, Yirui and Pollard, Steven and Conti, Luciano and Toselli, Mauro and Biella, Gerardo and Parkin, Georgina and Willatt, Lionel and Falk, Anna and Cattaneo, Elena and Smith, Austin}},
issn = {{1044-7431}},
keywords = {{Astrocytes/cytology; Biomarkers/metabolism; Cell Culture Techniques/methods; Cell Differentiation/physiology; Cell Division/physiology; Cell Line; Clone Cells; Embryonic Stem Cells/cytology; Fetus/cytology; Green Fluorescent Proteins/genetics; Humans; Neural Cell Adhesion Molecule L1/metabolism; Neurons/cytology; Oligodendroglia/cytology; Sialic Acids/metabolism; Transfection}},
language = {{eng}},
number = {{2}},
pages = {{58--245}},
publisher = {{Elsevier}},
series = {{Molecular and Cellular Neuroscience}},
title = {{Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture}},
url = {{https://lup.lub.lu.se/search/files/101077871/Long_term_tripotent_.pdf}},
doi = {{10.1016/j.mcn.2008.02.014}},
volume = {{38}},
year = {{2008}},
}